GenePrint® 10 System

10-Locus STR System for Cell Line Authentication

Cell line misidentification or contamination can derail your research. Fortunately, there is an easy way to confirm the identity of human cell lines in your laboratory: short tandem repeat (STR) analysis.

The GenePrint® 10 System is a 10-locus multiplex system designed to generate a multi-locus DNA profile by amplifying human genomic DNA extracted from cell pellets, cell culture enrichment media and by direct amplification from storage cards.

Economical, Efficient Cell Line Authentication

The GenePrint® 10 System includes all reagents required for successful identification and authentication of human cell lines, as well as detection of cell line contaminants; the small kit size and lower reaction volume makes GenePrint® 10 an economical choice for confirmation of cell line identity.

The GenePrint® 10 System:

Improves lab efficiencies with rapid-cycling and direct-amplification from storage card samples.
Simplifies data analysis and comparison using loci in concordance with publicly available databases.
Generates optimal heterozygote balance using up to 10ng of DNA template.
Delivers greater consistency in detecting low-level mixtures, contaminants and genetic aberrations.

Configuration of the GenePrint® 10 System

13670MB-W — *GenePrint*® 10 provides co-amplification and four-color detection of ten loci, TH01, TPOX, vWA, CSF1PO, D16S539, D7S820, D13S317, D5S818 plus Amelogenin and D21S11.

Ready to Use in Your Workflow

Improved Balance with Large Template Input

16131ta-v3 — Amplification of 10ng of human DNA using the *GenePrint*® 10 System and analyzed with a Spectrum Compact CE System using a 3.0kV, 4-second injection.

Compatible with Direct Amplification

11349ta-2-w — Amplification of DNA from the HT-29 cell line on a 1.2mm punch from an FTA® card. Amplified products were separated on an Applied Biosystems® 3130 Genetic Analyzer (3kV, 4-second injection). Inter- and intra-imbalance is expected when working with cell lines in storage cards.

Improved Balance with Large Template Input

11592ma-2-w — Amplification of 10ng of human DNA analyzed using an Applied Biosystems® 3130 Genetic Analyzer (3.0 kV, 4-second injection).

Detection Instrument Compatibility and Data Analysis

The GenePrint® 10 System is compatible with the Spectrum Compact CE System and the Applied Biosystems® SeqStudio, 3130, 3130xl, 3500 and 3500xL Genetic Analyzers.

You may need to optimize protocols including the amount of template DNA, cycle number, injection conditions and loading volume for your laboratory instrumentation.

Panels and bins text files are required for automatic assignment of genotypes using the GeneMapper® and GeneMarker® software and are available for download.

Download Panels and Bins

Protocols

Complete Protocol

GenePrint® 10 System for Use on the Applied Biosystems® Genetic Analyzers Technical ManualPDF (4726 KB) – English

GenePrint® 10 System for Use on the Spectrum Compact CE System Technical ManualPDF (15781 KB) – English

What's in the box?

Item	Part #	Size	Concentration	Available Separately
GenePrint® 10 5X Primer Pair Mix	B934A	1 × 250μl
GenePrint® 10 5X Master Mix	B935A	1 × 250μl
GenePrint® 10 Allelic Ladder Mix	B937A	1 × 50μl
2800M Control DNA	DD710A	1 × 25μl	10ng/μl	View Product
Internal Lane Standard 600	DG107A	1 × 150μl		View Product
Water, Amplification Grade	DW099A	1 × 1,250μl		View Product

SDS

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Certificate of Analysis

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Use Restrictions

Not For Medical Diagnostic Use.

Storage Conditions

See Protocol for detailed storage recommendations.

Resources

Articles

GenePrint® 10 System

STR Analysis for Human Cell Line Authentication

10-Locus STR System for Cell Line Authentication