Product and Technology Guides
Learn more about basic and advanced methods for cell biology, nucleic acid analysis, protein analysis and basic research in these educational resources. Each guide provides an overview of the basic science behind the methods and techniques described, as well as links to products and other helpful resources.
Cell Biology
Introduction to 3D Cell Culture
3D cell cultures are increasingly being used for basic research and drug discovery. Here you'll learn what 3D cell cultures are, why and how they are being used, and how to validate assays that work with 3D models.
ATP Assays
Learn how ATP assays work and how to choose the right assays for your needs.
Cell Line Authentication Resources
Misidentified cell lines can lead to lost resources, time and effort. Explore why cell line authentication is important and learn how to confirm the identity of your cells.
Choosing a Luciferase Reporter Assay
A comparison guide to help you select the right luciferase assay for your needs.
Interpreting Multiplex Cell Health Data
The CellTox Green Cytotoxicity Assay can be used with other cell health assays to provide richer information about why and how cells die. This interactive guide teaches you how to interpret the results from these experiments.
Live-Cell Kinetic Assays for Time Course Analysis
Learn how live-cell kinetic assay kits allow you to repeatedly measure one sample well over time, allowing you to get better data while saving time and precious samples.
Assays for Biochemical Targets
This selector tool is designed to help you quickly find the right Promega assay for your protein target of interest.
How to Measure Cellular Metabolism
Learn how to measure cellular metabolism using fast, simple, bioluminescent assays. Explore methods for glycolysis, glutaminolysis, lipogenesis, and more.
Cell Imaging
An overview of methods used for imaging studies in cell biology.
Reporter Genes and their Applications
How to use bioluminescent reporter genes to study eukaryotic gene expression and cellular events.
Apoptosis
An introduction to apoptotic cell death, including methods to confirm apoptosis.
Cell Viability Assay Guide
How to choose the right cell viability assay for your needs.
Transfection
A guide to transfection including protocols and optimization tips.
Nucleic Acid Analysis
qPCR and RT-qPCR
Molecular Cloning Guide
A guide to the fundamentals of molecular cloning, including restriction digestion, DNA ligation, vector dephosphorylation, and bacterial transformation.
How to Use Restriction Enzymes: A Resource Guide
Restriction enzymes 101, including reference information and links to restriction digest tools.
DNA Purification
An overview of DNA extraction methods for manual and automated purification needs.
Epigenetics
An introduction to the study of epigenetic changes in gene expression.
PCR Amplification
An overview of methods for PCR, RT-PCR and qPCR.
Protein Analysis
Protease Digestion for Mass Spectrometry
A collection of protocols for in-gel and in-solution protein digestion with trypsin, trypsin/lys-c or alternative proteases.
Protein Expression
An introduction to in vitro transcription and translation methods.
Protein Purification
An overview of common methods for protein purification.
A Proteomics Product Guide
An overview of products used for protein expression, purification and analysis.