CellTiter-Fluor™ Cell Viability Assay

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A Non-Lytic Fluorescence Assay That Measures the Relative Number of Viable Cells in Culture

  • Multiplex with many other luminescent assays
  • Normalizing data for live-cell numbers make results more comparable to other data

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Catalog number selected: G6080

$ 138.00
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CellTiter-Fluor™ Cell Viability Assay
10ml
$ 138.00
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How the Assay Works

The CellTiter-Fluor™ Cell Viability Assay is a non-lytic, single-reagent-addition fluorescence assay that measures the relative number of viable cells in a population. The assay is based on measurement of a conserved and constitutive protease activity within live cells and therefore serves as a biomarker of cell viability. The live-cell protease activity is restricted to intact viable cells and is measured using a fluorogenic, cell-permeant, peptide substrate (Gly-Phe-AFC). The substrate enters intact cells, where it is cleaved by the live-cell protease activity to generate a fluorescent signal proportional to the number of living cells. The live-cell protease becomes inactive upon loss of membrane integrity and leakage into the surrounding culture medium.

CellTiter-Fluor Cell Viability Assay chemistry.

Multiplexing with the CellTiter-Fluor™ Assay

 

The CellTiter-Fluor™ Assay also can be used in a single-well, sequential, multiplex format with other downstream assay chemistries to normalize data by cell number. Data from the assay can serve as an internal control and allow identification of errors resulting from cell clumping or compound cytotoxicity. In the example data on the right, ionomycin and PMA work cooperatively to stimulate NFAT-dependent gene expression; however, a higher concentration of ionomycin results in decreased expression. By simultaneously monitoring viability with the CellTiter-Fluor™ Assay, it is shown that the decrease in expression is due to a decrease in viable cells.

Single-Well Reporter and Toxicity Assay_10472MA

Luciferase reporter expression (ONE-Glo™ Assay) and cell viability (CellTiter-Fluor™ Assay) at increasing ionomycin concentration.


The CellTiter-Fluor™ Reagent was added to wells and viability measured after incubation for 30 minutes at 37°C. Caspase-Glo® 3/7 Reagent was added and luminescence measured after a 30-minute incubation (10,000 cells/well).

Multiplex of CellTiter-Fluor Assay and Caspase-Glo 3/7 Assay.

Specifications

You are viewing: G6080 Change Configuration

What's in the box?

Item Part # Size

GF-AFC Substrate

G608A 1 × 10μl

Assay Buffer

G610A 1 × 10ml

Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

Patent Pending.

Specifications

You are viewing: G6081 Change Configuration

What's in the box?

Item Part # Size

GF-AFC Substrate

G608A 5 × 10μl

Assay Buffer

G610A 5 × 10ml

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

Patent Pending.

Specifications

You are viewing: G6082 Change Configuration

What's in the box?

Item Part # Size

GF-AFC Substrate

G608B 2 × 50μl

Assay Buffer

G610B 2 × 50ml

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Patents and Disclaimers

Patent Pending.

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Petra

Petra

Germany