Cut Site:
G GTAC▼C
C▲CATG G
Usage Notes:
- Star activity may be observed with glycerol concentrations >12% or with enzyme:DNA ratios >25u/μg.
- KpnI recognizes the same DNA sequence but cleaves at a different nucleotide than its neoschizomer, Acc65I.
- KpnI is not sensitive to overlapping dcm methylation, unlike its neoschizomer, Acc65I.
Incubation Conditions: Buffer J. 37°C.
Source: Recombinant E. coli strain.
Storage Buffer: 10mM Tris-HCl (pH 7.4), 200mM KCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.