Caspase-Glo® 3/7 Assay System
Caspase Activity Assay for Apoptosis Detection
- High sensitivity caspase assay—requires fewer cells and less enzyme
- Simple add-mix-measure protocol—no sample preparation needed
- Easily scalable to 96-, 384- and 1,536-well plate formats
- Integrates seamlessly with the MyGlo™ Reagent Reader
Catalog Number:
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Size
$ 150.00
Catalog Number: G8090
$ 470.00
Catalog Number: G8091
$ 3,643.00
Catalog Number: G8093
$ 2,677.00
Catalog Number: G8092
$ 5,500.00
Catalog Number: MG1010
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Homogeneous Assay Measures Caspase-3/7 Activity
The Caspase-Glo® 3/7 Assay provides a proluminescent caspase-3/7 DEVD-aminoluciferin substrate and a proprietary thermostable luciferase in a reagent optimized for caspase-3/7 activity, luciferase activity and cell lysis. Adding the single Caspase-Glo® 3/7 Reagent in an "add-mix-measure" format results in cell lysis, followed by caspase cleavage of the substrate. This liberates free aminoluciferin, which is consumed by the luciferase, generating a "glow-type" luminescent signal that is proportional to caspase-3/7 activity.
The stabilized luciferase and proprietary buffer system improve assay performance across a wide range of assay conditions, and the assay is less likely to be affected by compound interference than fluorescent- or colorimetric-based assays. The caspase activity assay is designed for use with multiwell plate formats using either purified enzyme or cells in culture and can be multiplexed with other cell-based assays. The assay delivers excellent Z´-factor values in cell and purified enzyme models. Using the MyGlo™ Reagent Reader in conjunction with the ProNect™ Data Platform, you can visualize caspase activity with ease.
Need to measure caspase activity in 3D cultures? See our new Caspase-Glo® 3/7 3D Assay!
Simple Bioluminescent Detection of Caspase Activity
Luminescence Is Linear Over a Broad Range of Cell Numbers
Jurkat cells were treated with anti-Fas mAb for 4.5 hours to induce apoptosis or were left untreated. Caspase-Glo® 3/7 Reagent was added directly to the cells in 96-well plates and incubated for 1 hour before recording luminescence. Each point represents the average of 4 wells. The "no cell" blank control value has been subtracted from each.
Measure Dose Response to Apoptosis Inducers
Jurkat cells were seeded at 10,000 cells/well and treated for 20 hours with two compounds. Caspase-Glo® 3/7 Reagent was added to the cells and luminescence measured on the MyGlo™ Reagent Reader. The dose-response graph shows an apoptotic response to bortezomib with no apoptotic response to palbociclib.
Multiplex Caspase 3/7 with Cytotoxicity and Cell Viability Assays
Notes
Using 100μl of Caspase-Glo® Reagent per assay in a 96-well format, Cat.# G8090 provides sufficient reagents to perform 25 caspase activity assays; Cat.# G8091, 100 assays; Cat.# G8092 and G8093, 1,000 assays. Using 25μl of Caspase-Glo® Reagent per assay in a 384-well format, Cat.# G8090 provides sufficient reagents to perform 100 caspase activity assays; Cat.# G8091, 400 assays; Cat.# G8092 and G8093, 4,000 assays.
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size |
|---|---|---|
|
Caspase-Glo® 3/7 Buffer |
G810C | 1 × 2.5ml |
|
Caspase-Glo® 3/7 Substrate |
G811C | 1 × 1 bottle |
SDS
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Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
U.S. Pat. Nos. 7,148,030, 7,384,758, 7,666,987 and 8,071,328 and other patents and patents pending.
What's in the box?
| Item | Part # | Size |
|---|---|---|
|
Caspase-Glo® 3/7 Buffer |
G810A | 1 × 10ml |
|
Caspase-Glo® 3/7 Substrate |
G811A | 1 × 1 bottle |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
U.S. Pat. Nos. 7,148,030, 7,384,758, 7,666,987 and 8,071,328 and other patents and patents pending.
What's in the box?
| Item | Part # | Size |
|---|---|---|
|
Caspase-Glo® 3/7 Buffer |
G810A | 10 × 10ml |
|
Caspase-Glo® 3/7 Substrate |
G811A | 10 × 1 bottle |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
U.S. Pat. Nos. 7,148,030, 7,384,758, 7,666,987 and 8,071,328 and other patents and patents pending.
What's in the box?
| Item | Part # | Size |
|---|---|---|
|
Caspase-Glo® 3/7 Buffer |
G810B | 1 × 100ml |
|
Caspase-Glo® 3/7 Substrate |
G811B | 1 × 1 bottle |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
U.S. Pat. Nos. 7,148,030, 7,384,758, 7,666,987 and 8,071,328 and other patents and patents pending.
What's in the box?
| Item | Part # | Size | Available Separately |
|---|---|---|---|
MyGlo™ Reagent Reader
|
MG1000 | 1 × 1 each | |
CellTiter-Glo® 2.0 Reagent |
G9241 | 1 × 10ml | View Product |
SDS
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Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Resources
Articles
- Using a Real Time Kinetic Cytotoxicity Assay to Determine when to Detect Apoptosis by Caspase-3/7 Activation
- Elevated expression of miR-494-3p is associated with resistance to osimertinib in EGFR T790M-positive non-small cell lung cancer
- Enhanced extrinsic apoptosis of therapy-induced senescent cancer cells using a death receptor 5 (DR5) selective agonist
- Targeted zinc-finger repressors to the oncogenic HBZ gene inhibit adult T-cell leukemia (ATL) proliferation
- Metformin attenuates rotenone-induced oxidative stress and mitochondrial damage via the AKT/Nrf2 pathway
- Endogenous retroviruses mediate transcriptional rewiring in response to oncogenic signaling in colorectal cancer
- Reliable Automated Cytotoxicity Profiling for Chemistry-Focused Laboratories by Combining Multiplexed Cytotoxicity Assays with the CyBi®-Felix Liquid Handling Instrument
- Timing Your Apoptosis Assays
- Perform Multiplexed Cell-Based Assays on Automated Platforms
- High-Throughput Compound Profiling Using Tecan Instrumentation
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Recent publications that mention the use of this product.
