Plant Genotyping

Unlike animal cells, plant cells have rigid cell walls made of tough fibrous material and contain compounds that can interfere with DNA isolation as well as downstream applications, such as PCR. This makes isolating DNA from plant tissue more challenging. The Maxwell® RSC Plant DNA Kit is one example solution based on paramagnetic cellulose particles that consistently isolates high yields of DNA from a broad range of plant types in a safe and efficient manner. High-throughput automation is also available to address large numbers of samples that are processed in 96-well or 384-well plates.

The European Union (EU) and many other countries have policies for labeling or excluding products containing material from genetically modified organisms (GMO). GMO testing confirms the amount of GMO present in plants, seeds or foods and ensures compliance with EU and global regulations. PCR-based GMO detection methods are commonly used and rely on high yields of DNA with high purity for reproducible performance.

This blog article highlights the collaborative process of developing an automated method for plant gDNA isolation.

This article describes a protocol for extracting high-quality, RNA-free DNA from plants for GMO testing.