Biomarker Discovery

Biomarkers are used to measure cancer risk and indicate the presence of a tumor in a tissue. They reflect the molecular mechanisms driving the development of a tumor and could be used to influence treatment decisions. Circulating cell-free DNA (ccfDNA) has received recent attention as a promising biomarker present in blood plasma.

Circulating Cell-Free DNA

ccfDNA is an important biomarker for certain tumors. The quantity and sequences of ccfDNA molecules found in plasma can indicate the presence of a tumor and offer valuable insights into its characteristics.

ccfDNA 101

Research in circulating cell-free DNA (ccfDNA) has become a key area of interest in the study of normal and disease states. In this webinar, Promega scientist Douglas White discusses the history and basics of ccfDNA, such as:

What is it?
How do laboratories commonly extract ccfDNA?
What are typical applications for ccfDNA?

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Biopsy in a Tube

Read more about the promises of ccfDNA research.

ccfDNA Purification from Plasma

With such small, precious samples, it's important to purify high-quality, amplifiable DNA.

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Next-Generation Sequencing

Next-generation sequencing offers lots of valuable data from small samples, and nucleic acid quantitation is crucial for a successful run.

ccfDNA Enrichment through Size-Selective Purification

This article describes a magnetic bead-based size selection method that can be used to enrich for the ccfDNA component of a sample.

Read about Quantitation for NGS

Using the right quantitation method is important for ensuring successful NGS. Promega Product Specialist Adam Blatter wrote a series of blogs about choosing the right method.

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Measure intact, functional library concentration for NGS.

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