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BamHI

R6021_BamHI--2-500u_3
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  • Capable of digesting DNA in 15 minutes or less
  • Active and capable of digestion directly in GoTaq® Green Master Mix
  • Blue/white cloning-qualified, providing a higher level of quality control for enzymes used in cloning applications

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Catalog number selected: R6021

$ 58.00
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This product can be added to Helix

This product is available through the Promega Helix onsite stocking program. We offer numerous convenient solutions to meet your lab's needs.

Learn more about the Helix Collection »

This product is available under our Early Access program - Learn More
This product is available under our Catalog (FT) program - Learn More
BamHI
10u/µl/2,500u
$ 58.00
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Change Configuration

Cut Site:

G▼GATC  C
C   CTAG▲G

Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/custom/

Usage Notes:

  1. Star activity may be observed with glycerol concentrations >12%, enzyme:DNA ratio >25u/μg, low salt conditions or in the presence of Mn2+.
  2. Ends generated with BamHI can be directly ligated to ends generated with BglII, BclI and XhoII. These restriction sites are not regenerated in the ligation product.

Incubation Conditions: Buffer E. 37°C.

Source: Bacillus amyloliquefaciens H.

Storage Buffer: 10mM Tris-HCl (pH 7.4), 300mM KCl, 5mM MgCl2, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.

Percent Activity in 4-CORE® Buffer:

A B C D E MULTI-CORE™
75–100%* 75–100% 75–100% 50–75% 100% 75–100%

*Buffer A is not recommended due to potential star activity.

Frequency of Cutting:

λ Ad-2 ΦX174 pUC18 M13mp18 pBR322
5 3 0 1 1 1

Protocols

Complete Protocol

Specifications

You are viewing: R6021 Change Configuration

What's in the box?

Item Part # Size Concentration Available Separately

Buffer E 10X Buffer

 R005A 1 × 1ml

Bovine Serum Albumin, Acetylated

 R396D 1 × 150μl 10mg/ml View Product

BamHI

 R602A 1 × 2,500u

MULTI-CORE™ 10X Buffer

 R999A 1 × 0.25ml View Product

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Specifications

You are viewing: R6025 Change Configuration

What's in the box?

Item Part # Size Concentration Available Separately

Buffer E 10X Buffer

 R005A 1 × 1ml

Bovine Serum Albumin, Acetylated

 R396E 1 × 750μl 10mg/ml View Product

BamHI

 R602B 1 × 12,500u

MULTI-CORE™ 10X Buffer

 R999A 1 × 0.25ml View Product

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Resources

Citations

Other Resources

Similar Products

BclI

Restriction enzyme that cuts DNA at 50°C and leaves a 5´ overhang.

R6651

Frequently Used With

Blue/Orange Loading Dye, 6X

Marker dye for loading DNA samples into gel electrophoresis wells and tracking migration during electrophoresis.

G1881

LigaFast™ Rapid DNA Ligation System

Efficiently ligates DNA inserts into plasmid vectors in just 5–15 minutes at room temperature.

M8221, M8225

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