Prime-a-Gene® Labeling System

U1100_Prime-a-Gene--System_3
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Prime-a-Gene® Labeling System

The Prime-a-Gene® Labeling System provides a complete set of complementary reagents, including Labeling 5X Buffer that contains random synthetic hexadeoxynucleotide primers, for random-primed labeling of linear template DNA with radionucleotides. As little as 25ng of input DNA can be used to generate probes with specific activities >1 × 109cpm/μg.

Applications

  • Detection of single-copy genes and rare sequences.
  • Southern, Northern and dot blotting.
  • Plaque and colony lift hybridizations.

Specifications

What's in the box?

Item Part # Size Available Separately

DNA Polymerase I Large (Klenow) Fragment

M220A 1 × 150u View Product

Nuclease-Free Water

P119A 1 × 1,250μl View Product

Prime-a-Gene® dATP

U111A 1 × 40μl

Prime-a-Gene® dCTP

U112A 1 × 40μl

Prime-a-Gene® dGTP

U113A 1 × 40μl

Prime-a-Gene® dTTP

U114A 1 × 40μl

Labeling 5X Buffer

U115A 1 × 300μl

BSA, Nuclease-Free

U116A 1 × 75μl

Prime-a-Gene® Control DNA

U117A 1 × 25μl

Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

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