Trypsin Gold, Mass Spectrometry Grade

V5280_Trypsin-Gold-Mass-Spec-Grade--100ug_3
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Maximum Trypsin Digest Specificity, Extreme Resistance to Autolytic Digestion

  • Each lot qualified by mass spectrometry to ensures compatibility with your applications/instrumentation
  • Chymotrypsin activity eliminated for distinct, consistent data
  • Digest in gel, or in solution

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Catalog number selected: V5280

$ 125.00
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Trypsin Gold, Mass Spectrometry Grade
100μg
$ 125.00
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Excellent Specificity and Resistance to Autolytic Digestion

Trypsin Gold, Mass Spectrometry Grade, is manufactured to provide maximum specificity. Lysine residues in the porcine trypsin have been modified by reductive methylation, yielding a highly active and stable molecule that is extremely resistant to autolytic digestion. The specificity of the purified trypsin is further improved by TPCK treatment, which inactivates chymotrypsin. The treated trypsin is then purified by affinity chromatography and lyophilized to yield Trypsin Gold, Mass Spectrometry Grade.

Each lot of quality-tested Trypsin Gold, Mass Spectrometry Grade, is qualified for use with in-gel protein digestion and mass spectrometric analysis. For more information, see the Protein Purification and Analysis chapter of the Protocols and Applications Guide.

See our newest trypsin: Rapid Digestion-Trypsin and Rapid Digestion-Trypsin/Lys-C

Learn more about our custom options for this product at: www.promega.com/custom/

Spectrogram Showing Digestion with Trypsin Gold, Mass Spec Grade

Spectrogram of bovine carbonic anhydrase II protein digestion with Trypsin Gold, Mass Spectrometry Grade.

A 500ng sample of carbonic anhydrase II was separated by gel electrophoresis and digested with 500ng of Trypsin Gold, Mass Spectrometry Grade, overnight at 37°C.

Protocols

Complete Protocol

Specifications

What's in the box?

Item Part # Size

Trypsin Gold, Mass Spectrometry Grade

 V528A 1 × 100μg

SDS

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Certificate of Analysis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Resources

Citations

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AccuMAP® Low pH Protein Digestion Kits

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Frequently Used With

Arg-C Ultra, Mass Spec Grade

Endopeptidase that cleaves at the C-terminus of arginine residues, including Arg-Pro.

VA1831, VA1832

Lys-C, Mass Spec Grade

Cleavage site: C-terminal of Lys. Optimal pH: 7–9.

VA1170

Asp-N, Sequencing Grade

Cleavage site: N-terminal of Asp. Optimal pH: 4–9.

V1621

Glu-C, Sequencing Grade

Cleavage site: C-terminal of Glu. Optimal pH: 4–9.

V1651

rLys-C, Mass Spec Grade

Cleavage site: C-terminal of Lys. Optimal pH: 8–9.

V1671

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