ADP-Glo™ Kinase Assay

A Luminescent ADP Detection System for Kinases and Other ADP-Generating Enzymes

The ADP-Glo™ Kinase Assay measures ADP formed from a kinase reaction. The ADP is converted into ATP, which is used to generate light in a luciferase reaction. The luminescence generated correlates with kinase activity.

The assay is well suited for measuring the effects of chemical compounds on the activity of many purified kinases, making it ideal for primary screening as well as kinase selectivity profiling. Z′-factors >0.7 are routinely obtained. The stable luminescent signal allows batch plate processing without the need for strictly timed incubations.

Simple Kinase Assay Protocol

Detects Activity of a Range of Kinases

The ADP-Glo™ Kinase assay has a high dynamic range and produces a strong signal at low ATP-to-ADP conversion, making it well suited for screening low-activity kinases such as growth factor receptor tyrosine kinases. The assay can be used at ATP concentrations up to 1mM, important for kinases with high K_m values for ATP.

Use ADP-Glo™ Kinase Assay with Kinase Enzyme Systems to Simplify and Speed Kinase Profiling

Kinase Selectivity Profiling Systems

The Kinase Selectivity Profiling Systems provide kinase and substrate pairs grouped in single kinase families or as a general panel representative of the human kinome.

Kinase Profiling System	Cat #
Kinase Selectivity Profiling System: CMGC-1, CMGC-2	V6854, V6856
Kinase Selectivity Profiling System: AGC-1, AGC-2	V6858, V6910
Kinase Selectivity Profiling System: CAMK1, CAMK2	V6932, V6924
Kinase Selectivity Profiling System: TKL-1, STE-1	V6914, V6916
Kinase Selectivity Profiling System: Other/CK-1	V6914, V6916
Kinase Selectivity Profiling System: General Panel	V6928, V6929

Kinase Enzyme Systems

Each Kinase Enzyme System provides an individual kinase, substrate, and reaction buffer with optional ADP-Glo™ reagents. Kinase enzyme systems span the breadth of the human kinome; assays for quantifying the activity of over 170 kinases are available.

See all Kinase Enzyme Systems

Lipid Kinase Assay Reagents

Promega lipid kinase enzymes, substrates and detection systems provide a complete set of reagents for performing phosphoinositide lipid kinase (PIK) reactions using a luminescent ADP-detection platform, the ADP-Glo™ Kinase Assay. The reagents include purified human recombinant proteins of Class I PI3Ks, optimized reaction buffer and ready-to-use lipid kinase substrates.

Lipid Kinase Assays	Cat.#
ADP-Glo™ Kinase Assay with Lipid Kinase Substrates	V1781, V1791
PI3K Lipid Kinases	V1791

Kinase profiling is crucial for developing targeted kinase inhibitors. The ADP-Glo™ Assay, used with the Kinase Profiling Systems, Kinase Enzyme Systems or Lipid Kinase Assay Reagents, provides a convenient, simple method for profiling the effect of lead compounds on kinase activity.

Bioluminescence Resource Center

Learn more about how luminescence works, what makes it different from fluorescence and how you can use luminescence to fuel your own research.

Explore Now

Protocols

Complete Protocol

ADP-Glo™ Kinase Assay Technical ManualPDF (1566 KB) – English

Quick Protocols

ADP-Glo Kinase Assay Quick Protocol FB099PDF (69 KB) – English

What's in the box?

Item	Part #	Size
ADP-Glo™ Reagent	V912C	1 × 2ml
Ultra Pure ATP, 10mM	V915A	1 × 500μl
ADP, 10mM	V916A	1 × 500μl
Kinase Detection Reagent	V917A	1 × 4ml

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

Patents and Disclaimers

U.S. Pat. Nos. 7,741,067 and 8,361,739.

U.S. Pat. No. 7,700,310 and other patents and patents pending.

U.S. Pat. No. 8,183,007 and other patents and patents pending.

What's in the box?

Item	Part #	Size
ADP-Glo™ Reagent	V912A	1 × 5ml
Kinase Detection Buffer	V913A	1 × 10ml
Kinase Detection Substrate	V914A	1 × 1 each
Ultra Pure ATP, 10mM	V915A	1 × 500μl
ADP, 10mM	V916A	1 × 500μl

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

Patents and Disclaimers

U.S. Pat. Nos. 7,741,067 and 8,361,739.

U.S. Pat. No. 7,700,310 and other patents and patents pending.

U.S. Pat. No. 8,183,007 and other patents and patents pending.

What's in the box?

Item	Part #	Size
ADP-Glo™ Reagent	V912B	1 × 50ml
Kinase Detection Buffer	V913B	1 × 100ml
Kinase Detection Substrate	V914B	1 × 1 each
Ultra Pure ATP, 10mM	V915B	1 × 5ml
ADP, 10mM	V916B	1 × 5ml

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

Patents and Disclaimers

U.S. Pat. Nos. 7,741,067 and 8,361,739.

U.S. Pat. No. 7,700,310 and other patents and patents pending.

U.S. Pat. No. 8,183,007 and other patents and patents pending.

What's in the box?

Item	Part #	Size
ADP-Glo™ Kinase Assay	V9102	10 × 10,000 assays

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

Patents and Disclaimers

U.S. Pat. Nos. 7,741,067 and 8,361,739.

U.S. Pat. No. 7,700,310 and other patents and patents pending.

U.S. Pat. No. 8,183,007 and other patents and patents pending.

What's in the box?

Item	Part #	Size
ADP-Glo™ Reagent	V912B	10 × 50ml
Kinase Detection Buffer	V913B	10 × 100ml
Kinase Detection Substrate	V914B	10 × 1 each
Ultra Pure ATP, 10mM	V915B	10 × 5ml
ADP, 10mM	V916B	10 × 5ml

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

Patents and Disclaimers

U.S. Pat. Nos. 7,741,067 and 8,361,739.

U.S. Pat. No. 7,700,310 and other patents and patents pending.

U.S. Pat. No. 8,183,007 and other patents and patents pending.

Resources

Articles

Citations

Posters

Other Resources

Luminescence Resource Center | Bioluminescence Education

Similar Products

Kinase-Glo® Luminescent Kinase Assays

Determines activity of purified kinases by quantifying ATP remaining after a kinase reaction.

V6711, V6712, V6713, V6714, V6071, V6072, V6073, V6074, V3771, V3772, V3773, V3774

ADP-Glo™ Max Assay

Luminescent detection of ATPase or kinase activity.

V7001, V7002

ADP-Glo™ Kinase Assay

One Assay for All Your Kinase Screening Needs

A Luminescent ADP Detection System for Kinases and Other ADP-Generating Enzymes

Simple Kinase Assay Protocol

Detects Activity of a Range of Kinases

Use ADP-Glo™ Kinase Assay with Kinase Enzyme Systems to Simplify and Speed Kinase Profiling

Bioluminescence Resource Center

Protocols

Complete Protocol

Quick Protocols

Specifications

What's in the box?

SDS

Certificate of Analysis

Use Restrictions

Storage Conditions

Patents and Disclaimers

Specifications

What's in the box?

SDS

Certificate of Analysis

Use Restrictions

Storage Conditions

Patents and Disclaimers

Specifications

What's in the box?

SDS

Certificate of Analysis

Use Restrictions

Storage Conditions

Patents and Disclaimers

Specifications

What's in the box?

SDS

Certificate of Analysis

Use Restrictions

Storage Conditions

Patents and Disclaimers

Specifications

What's in the box?

SDS

Certificate of Analysis

Use Restrictions

Storage Conditions

Patents and Disclaimers

Resources

Articles

Citations

Posters

Other Resources

Similar Products

Kinase-Glo® Luminescent Kinase Assays

ADP-Glo™ Max Assay

Frequently Used With

GloMax® Explorer System

GloMax® Discover System

GloMax® Navigator System

Americas

Pacific Asia

Europe