The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer and supplemental reagents as needed. The TSSK2 Kinase Enzyme System contains:
- TSSK2 Kinase, 10μg (Human, recombinant, full-length); MW: ~66kDa
- Native Swine Myelin Basic Protein (MBP) Substrate, (1mg/ml)
- 5X Reaction Buffer; DTT, 0.1M
Recombinant full-length human TSSK2 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. TSSK2 is an intronless serine-threonine kinase that was originally detected on mouse chromosome 16. TSSK2 and TSSK1 share 72% overall amino acid identity and 83% identity in the kinase domain. Real-time PCR showed abundant TSSK2 expression in testis and lower levels in heart, brain, and placenta while Western blot analysis detected TSSK2 at the predicted molecular mass of 40kDa in human sperm and testis.
Application Note
NCBI Database Entry
The TSSK2 Kinase Enzyme System can be purchased with or without the ADP-Glo™ Kinase Assay reagents. Used together, the ADP-Glo™ Kinase Assay + Kinase Enzyme Systems provide a convenient method for profiling the effect of lead compounds on kinase activity. Assay advantages include broad dynamic range, ease of use and high sensitivity. Kinase Enzyme Systems are manufactured by SignalChem. Bulk quantities available upon request.
Use with ADP-Glo™ Kinase Assay
The ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects of chemical compounds on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.