TRS Custom Materials
Get started quickly by letting our team do the engineering for you. Whether it is CRISPR-generated reporter knock-in cells, traditional reporter vectors, or NanoBRET® Target Engagement tracers, we can create customized materials tailored to your research, so you can focus on generating the data you need. Leverage our expertise with a portfolio of innovative bioluminescent solutions designed specifically for your research.
Custom Reporter Knock-in Cell Lines
We leverage our expertise in HiBiT and other reporter technologies, combined with CRISPR-based engineering, to develop the most biologically relevant cell models for your research. By using endogenously inserted reporters, you can study genes under the control of a native promoter and epigenetic regulation, monitor proteins at physiological expression levels, and maintain stoichiometry with native interacting partners. This approach often results in improved assay windows, minimized artifacts and more accurate models of protein biology under physiologically relevant conditions.
With a proven track record of over 250 successful CRISPR projects across 50+ cell backgrounds, we guarantee precision and reliability in every project. Each cell line is delivered with a detailed data package to help you fully understand your results.
Custom Vectors
Custom vectors can be built to your specifications for a variety of research needs including NanoBRET® Target Engagement (TE), NanoBiT® and NanoBRET® Protein:Protein Interactions (PPI) Assays, and Bioassay development. Vectors can be provided as a stand-alone deliverable for you to do your own assay development or as a milestone step for further assay development. All vectors are compatible with transient expression, with formats for stable cell line generation also available.
Traditional reporter gene assays using:
- Firefly luciferase (luc2, luc2P)
- NanoLuc® luciferase (Nluc, NlucP, secNluc)
- Renilla luciferase (hRluc)
Create protein fusion constructs to express any wild-type or mutant protein of interest (POI) for NanoBiT® or NanoBRET® PPI or NanoBRET® TE assays:
- HaloTag® technology
- NanoLuc® luciferase technology
- NanoBiT® luciferase complementation components: LgBiT, SmBiT, HiBiT
