Protein Interactions

We offer a number of products for the analysis of protein interactions, including pull-down assays, BRET-based and complementation reporter-based systems.

GST pull-down systems are used in traditional methods based on cell extracts and/or cell-free expression. The HaloTag® Mammalian Pull-Down System is used to isolate and identify intracellular protein complexes from mammalian cells.

NanoBRET™ and NanoBiT® technologies provide methods for direct analysis of protein interactions in live cells. The NanoBRET™ System uses bioluminescence resonance energy transfer (BRET) in a proximity-based assay that detects protein interactions by measuring energy transfer from a bioluminescent protein donor to a fluorescent protein acceptor.

NanoBiT® assays rely on a two-subunit system based on NanoLuc® luciferase that can be used for intracellular detection of protein:protein interactions. The subunits are fused to proteins of interest, forming a functional enzyme that generates a bright, luminescent signal when the proteins interact.