Attention US and Canada Customers

Attention: Due to our annual physical inventory, orders received after 4PM CST on Tuesday, March 11th will be shipped according to the following schedule:
US Orders: Shipped on Saturday, March 15th with an expected arrival on Monday, March 17th.
Canada Orders: Shipped on Monday, March 17th with an expected arrival on Tuesday, March 18th.
We apologize for any inconvenience this may cause.
For any questions, please contact Customer Service at 1-800-356-9526.

HiBiT Protein Tagging System

Analyze and quantify proteins with high-sensitivity, tailored for your need.

HiBiT allows precise quantification and localization of proteins using simple, single-reagent bioluminescence methods and a high-affinity antibody. The adaptability and sensitivity provides new possibilities for studying protein function, degradation, secretion, and interactions at endogenous levels.

What Is HiBiT Tagging?

HiBiT is a small, 11-amino-acid, epitope tag capable of producing a bioluminescent signal when bound to its complementation partner, LgBiT. HiBiT has unparalleled versatility and convenience with options for simple and fast bioluminescent detection. The sensitive, high-affinity anti-HiBiT monoclonal antibody enables use in traditional immunoassays like Western blots and immunoprecipitation, making it adaptable to diverse workflows.

HiBiT technology offers an exceptional dynamic range, detecting low-abundance proteins at endogenous levels without requiring overexpression, thus preserving physiological relevance.

How does HiBiT compare with other epitope tags?

Get HiBiT Sequence

To obtain the HiBiT sequence, you must review the terms and conditions for use.

View this video to learn how HiBiT works.

HiBiT Products

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Lytic Detection

Sensitive quantification of HiBiT-tagged proteins in cell lysates or IP complexes.

View HiBiT Lytic Detection System
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Extracellular Protein Detection

Used with live cells for quantification of surface-expressed or secreted proteins.

View HiBiT Extracellular Detection System
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Intracellular Live-Cell Detection

Real-time bioluminescent quantification of intracellular HiBiT-tagged proteins in live cells.

View LgBiT mRNA Delivery System

Reduce Artifacts and Study Endogenously Expressed Proteins with CRISPR Technology

CRISPR insertion allows for monitoring of proteins under endogenous expression, removing artifacts observed from overexpression and more accurately modeling the biology. The small size of HiBiT makes insertion with CRISPR efficient, and the bioluminescence method enables sensitive, quantitative detection, even of proteins with low expression. The process removes the need for molecular cloning, reducing the time it takes to insert a knock-in tag from weeks to days.

Download CRISPR MethodLearn More about CRISPR Knock-ins
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Left Panel: Stabilizing Hif1a-HiBiT by incubation with the hypoxia mimetic 1,10-phenanthroline. Right Panel: Targeted degradation of HiBiT-BRD4 by incubating with compound dBET1. See figure details in this article: Quantifying Protein Abundance at Endogenous Levels.

Learn how to accurately measure protein surface expression with the Nano-Glo® HiBiT Extracellular and Lytic Assays in this whitepaper.