Lumit® Technology: Fast and Easy Immunodetection
Discover Lumit® Technology, a bioluminescence-based immunodetection system offering fast, sensitive, and accurate immunoassays for research and clinical applications.
What is Lumit® Technology?
Lumit® Technology is an advanced bioluminescence-based detection system designed to simplify immunoassay workflows while delivering fast, sensitive, and accurate results. It is based on NanoLuc® Luciferase structural complementation technology, enabling high-performance immunoassays for both research and clinical applications.
How Does Lumit® Technology Work?
Lumit® Immunoassays utilize split-luciferase technology, in which analyte sensors (e.g., antibodies, antigens, binding proteins) are chemically labeled with two luciferase subunits:
- Small bit (SmBiT)
- Large bit (LgBiT)
When the sensors bind to the target analyte, SmBiT and LgBiT come into close proximity, forming an active luciferase enzyme. Upon the addition of detection reagents, this enzyme generates a luminescent signal, enabling highly sensitive detection.
Watch this video to learn how Lumit® Immunoassays work.
Benefits of Lumit® Immunoassays
- No wash steps: Reduces variability and simplifies workflow.
- Faster results: Produces data in a fraction of the time.
- Higher sensitivity: Detects low-abundance targets with minimal background noise.
- Easily automated: Ideal for high-throughput labs processing large sample volumes.
Flexible Assay Formats
Lumit® Technology supports multiple assay configurations to meet diverse experimental needs:
Direct Detection: Primary antibodies labeled with SmBiT and LgBiT bind directly to the target analyte.
Indirect Detection: Secondary antibodies are labeled with SmBiT and LgBiT. Allows flexibility for assay development. (e.g., Lumit® Immunoassay Cellular Systems)
Competitive Binding: Requires antibody and tracer labeling. Suitable for small molecule detection and other specialized applications (e.g., Lumit® FcRn Binding Immunoassay).
More Applications
We continue to develop new applications for Lumit® Technology, including:
Biochemical Protein-Protein Interaction
Lumit® Anti-Tag Protein Interaction Reagents detect interactions of tagged proteins.
Double-Stranded RNA Detection
Lumit® dsRNA Detection Assay uses a peptide sensor for dsRNA detection.
Clinical Point-of-Care/High-Throughput Testing
Lumit® Flex is a three-part complementation system designed for clinical applications.
Lumit® Immunoassay Options
Prebuilt Assays
Kits to detect specific targets (e,g., cytokines, insulin, glucagon, phosphorylation activity)
DIY Assays
Antibody labeling kit and detection reagents to build your own immunoassays.
Clinical Assays
We can help you design, develop, and manufacture high-quality IVD assays.
Webinars
Tired of ELISA? It’s Time for Lumit® Immunoassays
Learn how Lumit® Immunoassays can simplify your workflow and yield faster results. We will present real examples for detecting cytokines, post-translational modifications, and antibody binding. You will also learn how to easily build your own Lumit® Immunoassay.
Bioluminescent Analyte Detection with Lumit® Immunoassays
Learn about Lumit® Technology and how it can be applied to a variety of assay formats. We will describe how bioluminescence makes immunoassays faster and easier, and how you can apply this technology to your research.
Resources
Guide to Immunoassays
New to immunoassays? Read this guide to learn about common types of immunoassays, their advantages/disadvantages, and how to choose the right immunoassay for your needs.
White Paper
Lumit® Immunoassays
Download this overview of Lumit® technology including examples of quantifying cytokines, protein phosphorylation, and antibody-Fc receptor binding.
White Paper
"Lumitizing" an ELISA Assay
Learn how to adapt tedious ELISA assays into a fast, sensitive Lumit® Immunoassay suitable for high-throughput applications.
Poster
Lumit® Flex Technology
Download this poster for an overview of Lumit® Flex Technology and example data: "Sensitive and Rapid Homogeneous Bioluminescent Platform for Clinical Applications"
Poster
Lumit® Technology
Download this poster for an overview of Lumit® Technology and example data: "A Novel, Bioluminescent, Homogeneous Immunoassay Platform for Analyte Detection"
Publications
See how Lumit® Technology is used in these peer-reviewed publications:
Swiatnicki, M. et al. (2022) Profiling oncogenic KRAS mutant drugs with a cell-based Lumit p-ERK immunoassay. SLAS Discov. 27, 249–257.
Hwang, B. et al. (2020) A homogeneous bioluminescent immunoassay to probe cellular signaling pathway regulation. Commun. Biol. 3, 8.
Kincaid, V. A. et al. (2022) Simple, rapid chemical labeling and screening of antibodies with luminescent peptides. ACS Chem. Biol. 17, 8, 2179–2187.
Hall, M. P. et al. (2021) Toward a point-of-need bioluminescence-based immunoassay utilizing a complete shelf-stable reagent. Anal. Chem. 93, 12, 5177–5184.
Torio, E. A. et al. (2022) Development of a rapid, simple, and sensitive point-of-care technology platform utilizing ternary NanoLuc. Front. Microbiol. 13, 970233.