Lumit® Cytokine Immunoassays for Biologics
Activating immune cells is the mechanism of action for many biologics, such as monoclonal antibodies and cell and gene therapy products. Potent cytokine assays that measure release from immune cells are important for lot release and characterization purposes. Traditionally, ELISAs have been used for cytokine quantitation, but the process is often tedious and slow. Learn how Lumit® Cytokine Immunoassays can help you get results faster.
In-Well Cytokine Assay—No Wash Steps!
Lumit® Cytokine Immunoassays can detect released cytokines directly in-well with cultured cells. With zero wash steps, you can get results in 70 minutes or less!
For a biotech that understands time is of significant value, the Lumit® Immunoassays are an essential way for us to gather reliable data fast so that we can reach conclusions and make decisions sooner.
Jonathan Chow, Principal Scientist, Corner Therapeutics
Same Results as ELISA, But Faster
Lumit® Cytokine Immunoassays achieve very similar results as ELISA but take significantly less time. Also, due to the expanded dynamic range of the assay, no sample dilutions are required.
Compare Lumit to ELISA quantification data of IL-6 release below.
View this video to learn how the Lumit® Cytokine Immunoassay protocol compares with ELISA.
The Lumit® IL-6 (Human) Immunoassay (left) and standard ELISA (right) were used to quantify IL-6 release from PBMCs. The results are similar, while the Lumit® Assay was completed in one fifth the time of the ELISA, with no transfer, wash steps or plate washer needed.
Easily Measure Bispecific T Cell Engager Potency
Lumit® Cytokine Immunoassays are suitable for use as bispecific T cell engager potency assays, such as in the example IL-2 and IFN-γ release data below. See more data in this poster: Novel Bioluminescent Bioassays for the Discovery and Development of Molecular and Cellular T -Cell Redirecting Cancer Therapy
Purified CD8+ T cells (effector cells) were combined with Raji B cells (target cells) and a serial dilution of Blincyto® (CD3/CD19 bispecific T cell engager). IL-2 and IFN-γ were detected with the corresponding Lumit® Cytokine Immunoassays to detect cytokine release from the effector cells.
Need a high-throughput solution?
You can easily detect cytokine release from hundreds of samples with Lumit® Cytokine Immunoassays. The increased throughput means you can run a full dose-response curve of your biologic. See high-throughput data in this poster" "Lumit® Cytokine Assay Automation for High-Throughput Screening".
Large Dynamic Range of Lumit® Cytokine Immunoassays
The wide linear range of Lumit® detection covers expected concentrations for most cell models. The performance characteristics of various Lumit® Cytokine Immunoassays are listed below.
| Immunoassay | Limit of Detection (LOD; 3 SD above background) |
Dynamic Range | Minimal Detectable Dose (MDD; 2 SD above background) |
|---|---|---|---|
| Lumit® IL-2 (Human) | 11.2pg/ml | 28.2pg/ml–25ng/ml | 7.2pg/ml |
| Lumit® IL-4 (Human) | 6.7pg/ml | 18.2pg/ml–25ng/ml | 4.5pg/ml |
| Lumit® IL-6 (Human) | 7.5pg/ml | 18.2pg/ml–25ng/ml | 5.0pg/ml |
| Lumit® IL-10 (Human) | 7.4pg/ml | 18.2pg/ml–25ng/ml | 4.9pg/ml |
| Lumit® IFN-γ (Human) | 1.7pg/ml | 7.3pg/ml–10ng/ml | 1.1pg/ml |
| Lumit® TNF-α (Human) | 2.9pg/ml | 18.2pg/ml–25ng/ml | 2.0pg/ml |
| Lumit® IL-12 (Human) | 4.5pg/ml | 18.2pg/ml–25ng/ml | 3.0pg/ml |
| Lumit® IL-1β (Human) | 10pg/ml | 22pg/ml–40ng/ml | 7pg/ml |
| Lumit® HMGB1 (Human) | 1ng/ml | 4–1,000ng/ml | — |
| Lumit® Active IL-18 (Human) | ≤10pg/ml | 11pg/ml–20ng/ml | — |
| Lumit® IL-8 (Human) | 1pg/ml | 7.29pg/ml–10ng/ml | 1pg/ml |
| Lumit® IL-17A (Human) | 3pg/ml | 18.2pg/ml–25ng/ml | 2pg/ml |
| Lumit® IFN-β (Human) | 5.3pg/ml | 18.2pg/ml–25ng/ml | 3.6pg/ml |
| Lumit® VEGF-A (Human) | 3pg/ml | 18.2pg/ml–25ng/ml | 2pg/ml |
| Lumit® MCP-1 (Human)— Coming soon | — | — | — |
| Lumit® TGF-β1 (Human)— Coming soon | — | — | — |
What’s in the Kits?
Each Lumit® Cytokine Immunoassay Kit contains the following:
- Recombinant human cytokine standard
- Anti-cytokine mAb-SmBiT
- Anti-cytokine mAb-LgBiT
- Lumit® Detection Substrate B
- Lumit® Detection Buffer B
Kits available for 100 assays, 500 assays or 1,000 assays. Contact your Promega representative to enquire about bulk pricing or sample availability.
Related Products
Lumit® Immunoassay Labeling Kit and Detection Reagents
Kit de marcaje de anticuerpos y reactivos de detección para ayudar a desarrollar inmunoensayos Lumit®.
VB2500, VB2010, VB2020, VB2030, VB4050, VB4060
Lumit® HMGB1 Human/Mouse Immunoassay
Cuantificación de HMGB1 mediante un protocolo add-mix-measure en menos de 90 minutos.
W6110, W6112
Lumit® IL-1β Human/Mouse Immunoassay
Cuantifica la activación del inflamasoma mediante la medición de la IL-1β liberada utilizando un protocolo sencillo y sin lavado.
W6010, W6011, W6012, W7010, W7011, W7012
Related Resources
- Lumit® Cytokine Assays: Interpolating Data with the GloMax® Discover
- Blog Article: Understanding Inflammation: A Faster, Easier Way to Detect Cytokines in Cells
- Blog Article: The Cytokine Storm: Why Some COVID-19 Cases are More Severe
- Webinar: Lumit® Immunoassays: An Easier Faster Method for Analyte Detection
- White Paper: Lumit® Immunoassays: A Rapid and Sensitive Method for Analyte Detection
- Application Note: Comparison of Lumit® and Cisbio HTRF® Assays for Measurement of Human TNF-α
- Application Note: CAR T Cell-Mediated Cytotoxicity and Cytokine Release in Response to Antigen Expression on Target Cells