TRS Custom Materials
Get started quickly by letting our team do the engineering for you. Whether it is CRISPR-generated reporter knock-in cells, traditional reporter vectors, or NanoBRET® Target Engagement tracers, we can create customized materials tailored to your research, so you can focus on generating the data you need. Leverage our expertise with a portfolio of innovative bioluminescent solutions designed specifically for your research.
Custom Reporter Knock-in Cell Lines
We leverage our expertise in HiBiT and other reporter technologies, combined with CRISPR-based engineering, to develop the most biologically relevant cell models for your research. By using endogenously inserted reporters, you can study genes under the control of a native promoter and epigenetic regulation, monitor proteins at physiological expression levels, and maintain stoichiometry with native interacting partners. This approach often results in improved assay windows, minimized artifacts and more accurate models of protein biology under physiologically relevant conditions.
With a proven track record of over 250 successful CRISPR projects across 50+ cell backgrounds, we guarantee precision and reliability in every project. Each cell line is delivered with a detailed data package to help you fully understand your results.
Why Promega?
- Full-Spectrum Licensing: We offer cell lines with our “knock-in” reporter tags under licenses granted by Broad Institute, Caribou Biosciences, SigmaMillipore and with access to patented Promega technologies. You can feel confident in your ability to operate without need to get other licenses. The terms of use are outlined in our Limited Use Label Licenses and do not require further licensing if the intended use falls within these licenses' terms. Promega operates under an agreement with the American Type Tissue Collection (ATCC), AdVec, and other parties to provide access to cell lines. If the intended cell lines are outside of our coverage we can work with you to ensure proper access if available.
- Reporter Expertise: Access our R&D know-how as the inventors of the HiBiT, HaloTag® and NanoLuc® technologies.
- Guaranteed Precision: Correct knock-in of your tag with no indels in the coding sequence for at least one allele.
- Format Options: Knock-ins offered as either cell lines or cell pools. Thaw-and-use cells also available to save you time culturing.
What to Expect
You Define:
- Target gene locus
- Reporter: HiBiT, HaloTag®, NanoLuc®, HiBiT-HaloTag
- Gene Terminus: N-terminus, C-terminus
- Preferred Cell Background: Authorized collection of cells from ATCC or provide your own cells
- Preference for LgBiT in the parental cell background
Our Experts
Generate the Reporter Insertion:
- Design and test guide RNAs for optimal insertion of tag via CRISPR
- Sort pool as single cells and expand for characterization
- Analyze top clones for luminescence and KI efficiency
Perform Best-in-Class Clone Validation:
- Sanger sequencing of target locus to verify correct insertion of the tag sequence
- Digital PCR (dPCR) to validate zygosity of knock-in
- Qualitative analysis including luminescence microscopy for subcellular localization and HiBiT blot for tagged target size (where possible)
Prior to delivery, clone cell banks are QC’d by confirming signal-to-background measurements post-thaw, tested to be free of mycoplasma and bacterial contamination, and tested for cell viability.
Custom Vectors
Custom vectors can be built to your specifications for a variety of research needs including NanoBRET® Target Engagement (TE), NanoBiT® and NanoBRET® Protein:Protein Interactions (PPI) Assays, and Bioassay development. Vectors can be provided as a stand-alone deliverable for you to do your own assay development or as a milestone step for further assay development. All vectors are compatible with transient expression, with formats for stable cell line generation also available.
Traditional reporter gene assays using:
- Firefly luciferase (luc2, luc2P)
- NanoLuc® luciferase (Nluc, NlucP, secNluc)
- Renilla luciferase (hRluc)
Create protein fusion constructs to express any wild-type or mutant protein of interest (POI) for NanoBiT® or NanoBRET® PPI or NanoBRET® TE assays:
- HaloTag® technology
- NanoLuc® luciferase technology
- NanoBiT® luciferase complementation components: LgBiT, SmBiT, HiBiT
