Apical-out intestinal organoids are a relatively simple method of gaining access to the apical cell surface and faced increasing popularity over the last few years. Apical-out organoids can thus be used for disease modelling to compare differing effects on the basolateral vs. the apical cell surface. However, these “inside-out” organoids die relatively quickly and cannot be propagated as long as their basal-out counterparts. Here we show that apical-out organoids have drastically reduced proliferative potential as evidenced immunohistochemical staining and the incorporation of the thymidine analogue EdU. This has an immense impact on research findings since the proliferation status affects several signalling cascades and ultimately lead to wrong interpretations of data.