Caspase-Glo® 9 Assay Systems

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A Luminescent Assay to Measure Caspase-9 Activity

  • Decreased assay time and less enzyme needed
  • Homogeneous “add-mix-measure” protocol amenable to automation

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Catalog number selected: G8210

$ 153.00
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Caspase-Glo® 9 Assay Systems
2.5ml
$ 153.00
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The Caspase-Glo® 9 Assay is a homogeneous luminescent assay that measures caspase-9 activity. The assay provides a proluminogenic caspase-9 substrate in a buffer system optimized for caspase activity, luciferase activity and cell lysis. The addition of a single Caspase-Glo® 9 Reagent in an "add-mix-read" format results in cell lysis, followed by caspase cleavage of the substrate and generation of a "glow-type" luminescent signal. The signal generated is proportional to the amount of caspase activity present. The Caspase-Glo® Reagent relies on the properties of a proprietary thermostable luciferase (Ultra-Glo™ Recombinant Luciferase), which generates the stable "glow-type" luminescent signal and improves performance across a wide range of assay conditions.

The system now includes a separate vial of a protease inhibitor, MG-132 Inhibitor, which may be used to reduce background, thus improving the performance of the Caspase-Glo® 9 Assay in cell-based applications.

Features and Benefits

  • The homogeneous "add-mix-read" protocol makes the assay easy to automate; simply add an equal volume of reagent to sample volume.
  • The low background luminescence results in excellent signal-to-noise ratios and superior sensitivity not achieved by other caspase formats, allowing assays to be performed in 96- or 384-well formats.
  • No sample preparation or manipulation required, and no extended incubation times are necessary as with fluorescent-based assays. Maximum sensitivity is achieved in as little as 0.5–1 hour.
  • The assay delivers excellent Z´ factors in cell and purified enzyme models.
  • The Caspase-Glo® 9 Assay uses a luminogenic substrate containing the LEHD sequence, which has been shown to be selective for caspase-9. The assay includes an optional proteasome inhibitor (MG-132), which when added to the Caspase-Glo® 9 Reagent significantly reduces nonspecific background in cell-based assays.
  • Multiplex with other cell-based assays from Promega.

Applications include caspase-9 activity determination, caspase-9 inhibition determination (IC50), and caspase-9 activation determination (EC50).

Notes: Using 100μl of Caspase-Glo® Reagent per assay in a 96-well format, Cat.# G8210 contains sufficient reagents for 25 assays; Cat.# G8211, 100 assays; Cat.# G8212, 1,000 assays, respectively. Using 25μl of Caspase-Glo® Reagent per assay in a 384-well format, Cat.# G8210 contains sufficient reagents for 100 assays; Cat.# G8211, 400 assays; Cat.# G8212, 4,000 assays, respectively.

Especificaciones

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Contenido

Item Part # Presentación

Caspase-Glo® 9 Substrate

G816C 1 × 1 bottle

Caspase-Glo® 9 Buffer

G822C 1 × 2.5ml

MG-132 Inhibitor

G932A 1 × 7.5μl

Certificado de Análisis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Condiciones de Almacenaje

BB

Patentes y Exclusiones

U.S. Pat. Nos. 7,148,030, 7,384,758, 7,666,987 and 8,071,328 and other patents and patents pending.

Especificaciones

You are viewing: G8211 Cambiar Configuración

Contenido

Item Part # Presentación

Caspase-Glo® 9 Substrate

G816A 1 × 1 bottle

Caspase-Glo® 9 Buffer

G822A 1 × 10ml

MG-132 Inhibitor

G932B 1 × 30μl

Certificado de Análisis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Condiciones de Almacenaje

BB

Patentes y Exclusiones

U.S. Pat. Nos. 7,148,030, 7,384,758, 7,666,987 and 8,071,328 and other patents and patents pending.

Especificaciones

You are viewing: G8212 Cambiar Configuración

Contenido

Item Part # Presentación

Caspase-Glo® 9 Substrate

G816B 1 × 1 bottle

Caspase-Glo® 9 Buffer

G822B 1 × 100ml

MG-132 Inhibitor

G932C 1 × 300μl

Certificado de Análisis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Condiciones de Almacenaje

BB

Patentes y Exclusiones

U.S. Pat. Nos. 7,148,030, 7,384,758, 7,666,987 and 8,071,328 and other patents and patents pending.

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