CytoTox 96® Non-Radioactive Cytotoxicity Assay
A Colorimetric Alternative to Radioactive Cytotoxicity Assays
- Measures release of LDH, an indicator of cytotoxicity
- Non-radioactive, time-saving assay
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The CytoTox 96® Non-Radioactive Cytotoxicity Assay is a colorimetric alternative to radioactive cytotoxicity assays. The assay quantitatively measures lactate dehydrogenase (LDH), a stable cytosolic enzyme that is released upon cell lysis, in much the same way as [51Cr] is released in radioactive assays. Released LDH in culture supernatants is measured with a 30-minute coupled enzymatic assay that results in the conversion of a tetrazolium salt (INT) into a red formazan product. The amount of color formed is proportional to the number of lysed cells. Visible wavelength absorbance data are collected using a standard 96-well plate reader. The assay can be used to measure membrane integrity for cell-mediated cytotoxicity assays in which a target cell is lysed by an effector cell, or to measure lysis of target cells by bacteria, viruses, proteins, chemicals, etc.
The CytoTox 96® Non-Radioactive Cytotoxicity Assay requires no radioactive waste disposal or [51Cr] and eliminates labeling of target cells prior to conducting experiments. Standard equipment can be used to collect absorbance (visible wavelength) data with a standard 96-well plate reader. The assay can be used for a variety of applications including measurement of 1) cell-mediated cytotoxicity; 2) chemical-mediated cytotoxicity; and 3) total cell number. A highly sensitive assay, it can reveal early, low-level damage to cell membranes that is often missed with other methodologies.
Protocols
Specifications
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Contenido
Item | Part # | Presentación |
---|---|---|
Substrate Mix |
G179A | 5 × 1 vial |
Assay Buffer |
G180A | 1 × 60ml |
LDH Positive Control |
G181A | 1 × 25μl |
Lysis Solution, 10X |
G182A | 1 × 5ml |
Stop Solution |
G183A | 1 × 65ml |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
Store LDH Positive Control, Lysis Solution (10X) and Stop Solution at 4°C.
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