G1780_CytoTox

A Colorimetric Alternative to Radioactive Cytotoxicity Assays

  • Measures release of LDH, an indicator of cytotoxicity
  • Non-radioactive, time-saving assay

Catalog Number:

Size

$ 389.00

Catalog Number: G1780

Add G1780 to Cart


to see your price

Overview
Protocols
Specifications
Resources
Related Products
Citations

The CytoTox 96® Non-Radioactive Cytotoxicity Assay is a colorimetric alternative to radioactive cytotoxicity assays. The assay quantitatively measures lactate dehydrogenase (LDH), a stable cytosolic enzyme that is released upon cell lysis, in much the same way as [51Cr] is released in radioactive assays. Released LDH in culture supernatants is measured with a 30-minute coupled enzymatic assay that results in the conversion of a tetrazolium salt (INT) into a red formazan product. The amount of color formed is proportional to the number of lysed cells. Visible wavelength absorbance data are collected using a standard 96-well plate reader. The assay can be used to measure membrane integrity for cell-mediated cytotoxicity assays in which a target cell is lysed by an effector cell, or to measure lysis of target cells by bacteria, viruses, proteins, chemicals, etc.

The CytoTox 96® Non-Radioactive Cytotoxicity Assay requires no radioactive waste disposal or [51Cr] and eliminates labeling of target cells prior to conducting experiments. Standard equipment can be used to collect absorbance (visible wavelength) data with a standard 96-well plate reader. The assay can be used for a variety of applications including measurement of 1) cell-mediated cytotoxicity; 2) chemical-mediated cytotoxicity; and 3) total cell number. A highly sensitive assay, it can reveal early, low-level damage to cell membranes that is often missed with other methodologies.

Specifications

Catalog Number:

Contenido

Item Part # Presentación

Substrate Mix

G179A 5 × 1 vial

Assay Buffer

G180A 1 × 60ml

LDH Positive Control

G181A 1 × 25μl

Lysis Solution, 10X

G182A 1 × 5ml

Stop Solution

G183A 1 × 65ml

Certificado de Análisis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Condiciones de Almacenaje

Store LDH Positive Control, Lysis Solution (10X) and Stop Solution at 4°C.

Resources

No related resources available

Product Citations

Recent publications that mention the use of this product.

View PubMed Citations