Lumit® TNF-α (Human) Immunoassay

Quantify Cytokine Release Without Tedious Wash Steps

The Lumit® TNF-α Immunoassay quantitatively measures released TNF-α in cell culture samples using a simple, no-wash protocol. Just add labeled antibodies to the sample, add detection reagent and read luminescent signal using a standard plate-reading luminometer. The entire protocol is completed in less than 70 minutes! The assay can be used directly on cells in culture or culture medium transferred to a separate assay plate.

How the Lumit® TNF-α Immunoassay Works

Primary antibodies to TNF-α were selected for their specific and sensitive detection and labeled with the LgBiT and SmBiT subunits of NanoBiT® Luciferase. In the presence of TNF-α, the subunits are brought together to form an active luciferase enzyme. Addition of optimized substrate generates a bright luminescent signal proportional to TNF-α levels.

Simple One-Plate Protocol, No Wash Steps

Large Dynamic Range of Lumit® TNF-α Detection

The pg/ml to ng/ml linear range of Lumit® detection covers expected concentrations for most cell models.

Assay	TNF-α Human Immunoassay
Limit of Detection (LOD)	2.9pg/ml (3 SD above background)
Dynamic Range	18.2pg/ml – 25ng/ml
Minimal Detectable Dose (MDD)	2.0pg/ml (2 SD above background)
Assay Time	70 minutes
Sample Type	Cell culture supernatants

SD=Standard Deviation

Sensitive Detection of TNF-α Without Sample Dilution

When treated with a Cell Stimulation Cocktail (CSC), human PBMCs release TNF-α. After treatment for 24, 48 or 72 hours, Lumit® TNF-α Human Immunoassay reagents were added directly to cell wells to measure TNF-α release. No sample dilution was required to measure quantities of TNF-α release.

Multiple Cytokine Detection by Split-Sample Analysis

Split-sample analysis for detection of IL-2, IL-6, IFN-γ and TNF-α using corresponding Lumit™ Cytokine Immunoassay.18001ma — Human PBMCs were plated in a 96-well plate in 100µl and treated for 24 hours with cell stimulation cocktail (CSC), lipopolysaccharide (LPS) or R848. Four 20μl aliquots were transferred to a 384-well plate for detection of IL-2, IL-6, IFN-γ and TNF-α with the corresponding Lumit® Cytokine Immunoassay. This simple approach for profiling cytokine release can be applied to multiple cell models.

Featured Application Note

CAR T cell-mediated cytotoxicity and cytokine release in response to antigen expression on target cells

Explore a multiplexing approach that unlocks the full potential of CAR T cell therapy with our Lumit® IFN-γ and TNF-α (Human) Immunoassays.

View Application Note

Explore Additional Cytokine Detection Assays

We offer a growing portfolio of assays to detect cytokines in cell culture samples.

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Please enquire about early access to new assays in development.

For a biotech that understands time is of significant value, the Lumit® Immunoassays are an essential way for us to gather reliable data fast so that we can reach conclusions and make decisions sooner.

Jonathan Chow, Principal Scientist, Corner Therapeutics

Protocolos

Complete Protocol

Lumit™ TNF-α (Human) Immunoassay Technical ManualPDF (2001 KB) – English

Contenido

Item	Part #	Presentación	Concentración
Lumit® Detection Substrate B	VB405A	1 × 160μl
Lumit® Detection Buffer B	VB406A	1 × 3.2ml
Human TNF-α Standard	W137A	1 × 25μl	10μg/ml
Anti-hTNF-α mAb-SmBiT, 1000X	W138A	1 × 15μl
Anti-hTNF-α mAb-LgBiT, 1000X	W139A	1 × 15μl

SDS

Search for SDS

Certificado de Análisis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Condiciones de Almacenaje

Patentes y Exclusiones

U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.

U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 10,648,971 and other patents and patents pending.

Contenido

Item	Part #	Presentación	Concentración
Lumit® Detection Substrate B	VB405A	5 × 160μl
Lumit® Detection Buffer B	VB406A	5 × 3.2ml
Human TNF-α Standard	W137A	1 × 25μl	10μg/ml
Anti-hTNF-α mAb-SmBiT, 1000X	W138A	5 × 15μl
Anti-hTNF-α mAb-LgBiT, 1000X	W139A	5 × 15μl

SDS

Search for SDS

Certificado de Análisis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Condiciones de Almacenaje

Patentes y Exclusiones

U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.

U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 10,648,971 and other patents and patents pending.

Contenido

Item	Part #	Presentación	Concentración
Lumit® Detection Substrate B	VB405B	1 × 1.25ml
Lumit® Detection Buffer B	VB406B	1 × 25ml
Human TNF-α Standard	W137A	1 × 25μl	10μg/ml
Anti-hTNF-α mAb-SmBiT, 1000X	W138B	1 × 150μl
Anti-hTNF-α mAb-LgBiT, 1000X	W139B	1 × 150μl

SDS

Search for SDS

Certificado de Análisis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Condiciones de Almacenaje

Patentes y Exclusiones

U.S. Pat. No. 8,809,529, European Pat. No. 2635582, Japanese Pat. No. 5889910 and other patents and patents pending.

U.S. Pat. Nos. 9,797,889, 9,797,890, 10,107,800 and 10,648,971 and other patents and patents pending.

Recursos

Artículos

Posters

Homogeneous, Bioluminescent Cytokine Assay Applied to a Monocyte Activation Test for Pyrogen Detection

Otros

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Lumit® TNF-α (Human) Immunoassay

No-Wash Immunoassay for TNF-α Detection

Quantify Cytokine Release Without Tedious Wash Steps

How the Lumit® TNF-α Immunoassay Works

Simple One-Plate Protocol, No Wash Steps

Large Dynamic Range of Lumit® TNF-α Detection

Sensitive Detection of TNF-α Without Sample Dilution

Multiple Cytokine Detection by Split-Sample Analysis

Featured Application Note

CAR T cell-mediated cytotoxicity and cytokine release in response to antigen expression on target cells

Explore Additional Cytokine Detection Assays

Protocolos

Complete Protocol

Especificaciones

Contenido

SDS

Certificado de Análisis

Use Restrictions

Condiciones de Almacenaje

Patentes y Exclusiones

Especificaciones

Contenido

SDS

Certificado de Análisis

Use Restrictions

Condiciones de Almacenaje

Patentes y Exclusiones

Especificaciones

Contenido

SDS

Certificado de Análisis

Use Restrictions

Condiciones de Almacenaje

Patentes y Exclusiones

Recursos

Artículos

Posters

Otros

Productos Similares

Lumit® IL-1β Human/Mouse Immunoassay

Lumit® HMGB1 Human/Mouse Immunoassay

Lumit® Immunoassay Labeling Kit and Detection Reagents

Lumit® IFN-γ (Human) Immunoassay

Lumit® IL-2 (Human) Immunoassay

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LDH-Glo™ Cytotoxicity Assay

T Cell Activation Bioassay

GloMax® Discover System

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