GDP-Fucose, 50mM, and GDP-Mannose, 100mM, are ultra pure GDP-sugar substrates designed for use with the GDP-Glo™ Glycosyltransferase Assay—a bioluminescent assay for detecting the activity of glycosyltransferases that use GDP-sugars as donor substrates.
Glycosylation reactions catalyzed by glycosyltransferases are central to many biological processes, including cell:cell interactions, cell signaling and bacterial cell wall biosynthesis. Glycosyltransferases transfer sugar from a nucleotide-glycosyl donor (e.g., GDP-Fucose and GDP-Mannose) to an acceptor molecule. In a glycosyltransferase reaction, the GDP moiety is released as a product; therefore, an assay that detects GDP can be used to monitor the activity of all the GDP-sugar-utilizing glycosyltransferases (e.g, Fucosyltransferases).