Kinase Selectivity Profiling Systems

Profile Lead Compounds in Hours, Not Days

The Kinase Selectivity Profiling Systems are ideal for profiling lead-compound kinase inhibitors against a broad panel of kinases to better understand inhibitor activity and obviate any off-target effects. Each system includes kinase and substrate pairs organized in an easy-to-use 8-tube strip format optimized for fast and simple kinase profiling reactions.

The General Panel Kinase Selectivity Profiling System contains 24 kinases arranged in three 8-well strips, the corresponding substrates and cofactors, and kinase reaction buffer.

Optimized for Use With ADP-Glo™ Kinase Assay

The Kinase Selectivity Profiling Systems rely on the luminescent ADP-Glo™ Kinase Assay technology to characterize kinase selectivity. The luminescence generated by the ADP-Glo™ Kinase Assay is proportional to ADP concentration and correlates with kinase activity.

Each Kinase Selectivity Profiling System is available with or without ADP-Glo™ Assay reagents.

Simple, Automation-Friendly Assay, No Time-Consuming Optimization

Simply dilute the stock solutions provided, and you’re ready to go. Two quick, one-step dilutions provide working stocks of kinases and substrate/cofactor solutions.

Concentrated kinases and substrates/cofactor stocks are first diluted and then combined with test compound in a 384-well plate. After a 1-hour incubation, the ADP-Glo™ Assay is performed to measure kinase activity.

Kinase profiling method overview 12059ma-w

Assays can be performed manually, or automated on a liquid-handler.

Single-Dose Profiling

Each Kinase Selectivity Profiling System provides enough material to profile up to 20 compounds at a single dose or create a dose response for two compounds against eight kinases at once.

12060MB-W — **Example single-dose inhibitor profiles of eight compounds created using the Kinase Selectivity Profiling System—TK-3.** Kinase assays were assembled in the presence of the indicated compounds (1μM final concentration). Kinase activity was quantified using the ADP-Glo™ Kinase Assay. The percent activity of each kinase in the presence of each compound is shown.

Custom Kinase Assay Strips Available

If you need a different combination of kinases than those provided in our catalog, you can design your own custom profiling system using our kinase selector tool.

Build Your Own Kinase Profiling System

Protocolos

Complete Protocol

Kinase Selectivity Profiling System: General PanelPDF (1046 KB) – English

SDS

Search for SDS

Certificado de Análisis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Condiciones de Almacenaje

See Protocol for detailed storage recommendations.

Contenido

Item	Part #	Presentación
Kinase Selectivity Profiling System: General Panel	V6928	1 × 24 × 50 reactions
ADP-Glo™ Kinase Assay	V6930	3 × 400 assays

SDS

Search for SDS

Certificado de Análisis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Condiciones de Almacenaje

See Protocol for detailed storage recommendations.

Patentes y Exclusiones

U.S. Pat. Nos. 7,741,067 and 8,361,739.

U.S. Pat. No. 8,183,007 and other patents and patents pending.

U.S. Pat. No. 7,700,310 and other patents and patents pending.

Recursos

Otros

Automating the Kinase Profiling Systems

Kinase Selectivity Profiling Systems—General Panel

Selectivity Screening of Library Compounds for Effect on Panels of Kinases

Profile Lead Compounds in Hours, Not Days

Optimized for Use With ADP-Glo™ Kinase Assay

Simple, Automation-Friendly Assay, No Time-Consuming Optimization

Assays can be performed manually, or automated on a liquid-handler.

Single-Dose Profiling

Custom Kinase Assay Strips Available

Protocolos

Complete Protocol

Especificaciones

SDS

Certificado de Análisis

Use Restrictions

Condiciones de Almacenaje

Especificaciones

Contenido

SDS

Certificado de Análisis

Use Restrictions

Condiciones de Almacenaje

Patentes y Exclusiones

Recursos

Otros

Kinase Selectivity Profiling Systems—AGC

Kinase Selectivity Profiling Systems—CAMK1 and CAMK2

Kinase Selectivity Profiling Systems—CMGC

Kinase Selectivity Profiling Systems—TK1, TK2, TK3, TK4

Kinase Selectivity Profiling Systems—STE and TKL

Kinase Selectivity Profiling Systems—Other

Kinase Selectivity Profiling Systems—General Panel

Selectivity Screening of Library Compounds for Effect on Panels of Kinases

Profile Lead Compounds in Hours, Not Days

Optimized for Use With ADP-Glo™ Kinase Assay

Simple, Automation-Friendly Assay, No Time-Consuming Optimization

Assays can be performed manually, or automated on a liquid-handler.

Single-Dose Profiling

Custom Kinase Assay Strips Available

Protocolos

Complete Protocol

Especificaciones

SDS

Certificado de Análisis

Use Restrictions

Condiciones de Almacenaje

Especificaciones

Contenido

SDS

Certificado de Análisis

Use Restrictions

Condiciones de Almacenaje

Patentes y Exclusiones

Recursos

Otros

Productos Similares

Kinase Selectivity Profiling Systems—AGC

Kinase Selectivity Profiling Systems—CAMK1 and CAMK2

Kinase Selectivity Profiling Systems—CMGC

Kinase Selectivity Profiling Systems—TK1, TK2, TK3, TK4

Kinase Selectivity Profiling Systems—STE and TKL

Kinase Selectivity Profiling Systems—Other

Usado con frecuencia junto con

ADP-Glo™ Kinase Assay

GloMax® Discover System

GloMax® Navigator System

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