ADP-Glo™ Kinase Assay with Lipid Kinase Substrates

Ready-to-Use Lipid Kinase Substrates and Detection Reagents

The ADP-Glo™ Kinase Assay with PI:3PS (Cat.# V1781, V1782) contains PI:3PS Lipid Kinase Substrate (0.25mg) and the ADP-Glo™ Kinase Assay.

The ADP-Glo™ Kinase Assay with PIP2:3PS (Cat.# V1791, V1792) contains PIP2:3PS Substrate (0.25mg) and the ADP-Glo™ Kinase Assay.

These products are designed for use in screening assays with purified PI3K Class I lipid kinases. The lipid substrates are supplied as frozen small unilamellar vesicles containing a mixture of phosphatidylinositol (PI) or phosphoinositol-4,5-bisphosphate (PIP2) at a 1:3 ratio with phosphatidylserine (PS) as carrier lipid. The PIP2:3PS substrate is optimized for use with class I PI3Ks. The PI:3PS substrate provides a universal PI lipid kinase substrate.

The ADP-Glo™ Kinase Assay with PI:3PS and the ADP-Glo™ Kinase Assay with PIP2:3PS are components of the PI3K-Glo™ Class I Profiling Kit (Cat.# V1690).

Lipid Kinase Assay Principle

The lipid kinase reaction is performed by incubating lipid substrate (PI:3PS or PIP2:3PS) with a recombinant enzyme and ATP, and kinase activity is measured using the ADP-Glo™ Kinase Assay. After the kinase reaction, an ATP-depletion reagent is added to terminate the lipid kinase reaction and deplete any remaining ATP, leaving only ADP. Next, a detection reagent is added to simultaneously convert ADP to ATP, which is converted to a luminescent signal using a coupled luciferase/luciferin reaction.

Principle of the ADP-Glo™ Lipid Kinase Assay.

Protocolos

Complete Protocol

ADP-Glo™ Lipid Kinase Assay Technical ManualPDF (1492 KB) – English

Contenido

Item	Part #	Presentación	Available Separately
PI:3PS Substrate, 1mg/ml PI	V171A	1 × 0.5ml	View Product
Lipid Dilution Buffer, 10X	V180A	1 × 1ml
MgCl₂, 1M	V330A	1 × 1ml
ADP-Glo™ Reagent	V912A	1 × 5ml
Kinase Detection Buffer	V913A	1 × 10ml
Kinase Detection Substrate	V914A	1 × 1 each
Ultra Pure ATP, 10mM	V915A	1 × 500μl
ADP, 10mM	V916A	1 × 500μl

SDS

Search for SDS

Certificado de Análisis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Condiciones de Almacenaje

See Technical Manual for detailed storage recommendations.

Patentes y Exclusiones

U.S. Pat. Nos. 7,741,067 and 8,361,739.

U.S. Pat. No. 7,700,310 and other patents and patents pending.

U.S. Pat. No. 8,183,007 and other patents and patents pending.

Contenido

Item	Part #	Presentación	Available Separately
ADP-Glo™ Reagent	V912B	1 × 50ml
Kinase Detection Buffer	V913B	1 × 100ml
Kinase Detection Substrate	V914B	1 × 1 each
Ultra Pure ATP, 10mM	V915B	1 × 5ml
ADP, 10mM	V916B	1 × 5ml
PI:3PS Substrate, 1mg/ml PI	V171A	10 × 0.5ml	View Product
Lipid Dilution Buffer, 10X	V180A	5 × 1ml
MgCl₂, 1M	V330A	1 × 1ml

SDS

Search for SDS

Certificado de Análisis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Condiciones de Almacenaje

See Technical Manual for detailed storage recommendations.

Patentes y Exclusiones

U.S. Pat. Nos. 7,741,067 and 8,361,739.

U.S. Pat. No. 7,700,310 and other patents and patents pending.

U.S. Pat. No. 8,183,007 and other patents and patents pending.

Contenido

Item	Part #	Presentación	Available Separately
PIP2:3PS Substrate, 1mg/ml PIP2	V170A	1 × 0.25ml	View Product
Lipid Dilution Buffer, 10X	V180A	1 × 1ml
MgCl₂, 1M	V330A	1 × 1ml
ADP-Glo™ Reagent	V912A	1 × 5ml
Kinase Detection Buffer	V913A	1 × 10ml
Kinase Detection Substrate	V914A	1 × 1 each
Ultra Pure ATP, 10mM	V915A	1 × 500μl
ADP, 10mM	V916A	1 × 500μl

SDS

Search for SDS

Certificado de Análisis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Condiciones de Almacenaje

See Technical Manual for detailed storage recommendations.

Patentes y Exclusiones

U.S. Pat. Nos. 7,741,067 and 8,361,739.

U.S. Pat. No. 7,700,310 and other patents and patents pending.

U.S. Pat. No. 8,183,007 and other patents and patents pending.

Contenido

Item	Part #	Presentación	Available Separately
ADP-Glo™ Reagent	V912B	1 × 50ml
Kinase Detection Buffer	V913B	1 × 100ml
Kinase Detection Substrate	V914B	1 × 1 each
Ultra Pure ATP, 10mM	V915B	1 × 5ml
ADP, 10mM	V916B	1 × 5ml
PIP2:3PS Substrate, 1mg/ml PIP2	V170A	10 × 0.25ml	View Product
Lipid Dilution Buffer, 10X	V180A	5 × 1ml
MgCl₂, 1M	V330A	1 × 1ml

SDS

Search for SDS

Certificado de Análisis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Condiciones de Almacenaje

See Technical Manual for detailed storage recommendations.

Patentes y Exclusiones

U.S. Pat. Nos. 7,741,067 and 8,361,739.

U.S. Pat. No. 7,700,310 and other patents and patents pending.

U.S. Pat. No. 8,183,007 and other patents and patents pending.

ADP-Glo™ Kinase Assay with Lipid Kinase Substrates

Substrate and Assay Reagents for Detecting Class I PI3K Activity

Ready-to-Use Lipid Kinase Substrates and Detection Reagents

Lipid Kinase Assay Principle

Protocolos

Complete Protocol

Especificaciones

Contenido

SDS

Certificado de Análisis

Use Restrictions

Condiciones de Almacenaje

Patentes y Exclusiones

Especificaciones

Contenido

SDS

Certificado de Análisis

Use Restrictions

Condiciones de Almacenaje

Patentes y Exclusiones

Especificaciones

Contenido

SDS

Certificado de Análisis

Use Restrictions

Condiciones de Almacenaje

Patentes y Exclusiones

Especificaciones

Contenido

SDS

Certificado de Análisis

Use Restrictions

Condiciones de Almacenaje

Patentes y Exclusiones

ADP-Glo™ Kinase Assay with Lipid Kinase Substrates

Substrate and Assay Reagents for Detecting Class I PI3K Activity

Ready-to-Use Lipid Kinase Substrates and Detection Reagents

Lipid Kinase Assay Principle

Protocolos

Complete Protocol

Especificaciones

Contenido

SDS

Certificado de Análisis

Use Restrictions

Condiciones de Almacenaje

Patentes y Exclusiones

Especificaciones

Contenido

SDS

Certificado de Análisis

Use Restrictions

Condiciones de Almacenaje

Patentes y Exclusiones

Especificaciones

Contenido

SDS

Certificado de Análisis

Use Restrictions

Condiciones de Almacenaje

Patentes y Exclusiones

Especificaciones

Contenido

SDS

Certificado de Análisis

Use Restrictions

Condiciones de Almacenaje

Patentes y Exclusiones

Productos Similares

PI3K-Glo™ Class I Profiling Kit

ADP-Glo™ Max Assay

ADP-Glo™ Kinase Assay

Lipid Kinase Substrates

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