The Kinase Enzyme Systems include a recombinant kinase enzyme, a substrate appropriate for the enzyme, a reaction buffer and supplemental reagents as needed. The NEK9 Kinase Enzyme System contains:
- NEK9 Kinase, 10μg (Human, recombinant; amino acids 1–979; deleted fragment of 346–731). MW: ~115kDa
- Native Swine Myelin Basic Protein (MBP) Substrate, (1mg/ml)
- 5X Reaction Buffer, 0.1M DTT
Recombinant human NEK9 (1–979; deleted fragment of 346–731) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. NEK9 is a member of the NEK family and has high homology to NEK1, NEK3 and NEK4. NEK9 is activated during mitosis and binds specifically to RAN GTPase and is a substrate for CDC2 phosphorylation. NEK9 plays a role in the control of mitotic progression and is regulated by CDC2 and RAN GTPase. Overexpression of both active and inactive variants of NEK9 is toxic to cells and inhibits cell division causing abnormal nuclear morphologies.
Application Note
NCBI Database Entry
The NEK9 Kinase Enzyme System can be purchased with or without the ADP-Glo™ Kinase Assay reagents. Used together, the ADP-Glo™ Kinase Assay + Kinase Enzyme Systems provide a convenient method for profiling the effect of lead compounds on kinase activity. Assay advantages include broad dynamic range, ease of use and high sensitivity. Kinase Enzyme Systems are manufactured by SignalChem. Bulk quantities available upon request.
Use with ADP-Glo™ Kinase Assay
The ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is a substrate in a reaction catalyzed by Ultra-Glo™ Luciferase that produces light. The luminescent signal positively correlates with ADP amount and kinase activity. The assay is well suited for measuring the effects of chemical compounds on the activity of a broad range of purified kinases, making it ideal for both primary screening as well as kinase selectivity profiling. The ADP-Glo™ Kinase Assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) using up to 1mM ATP.