pGEM®-7Zf(+) Vector

P2251_pGEM--7Zf----Vector--20ug_3
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Standard Cloning Vector and Template for In vitro Transcription and Circular ssDNA Production

  • Blue/white screening for easily identifying recombinant clones
  • In vitro transcription from SP6 and T7 RNA polymerase promoters flanking the multiple cloning region
  • Multiple cloning region provides a convenient selection of restriction sites for cloning

Presentación

Catalog number selected: P2251

$ 156.00
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pGEM®-7Zf(+) Vector
20μg
$ 156.00
Your price: Acceder

The pGEM®-7Zf(+) Vector contains SP6 and T7 RNA polymerase promoters flanking a region of multiple cloning sites within the α-peptide coding region of β-galactosidase. This arrangement of promoters means both sense and antisense RNA can be produced. Insertional inactivation of the α-peptide identified recombinant clones by color screening on indicator plates when using appropriate E. coli strains (e.g., JM109). The multiple cloning region is unique and includes restriction sites for ApaI, AatII, SphI, XbaI, XhoI, EcoRI, KpnI, SmaI, ClaI, HindIII, BamHI, SacI, BstXI and NsiI.

Reference

  1. Yanisch-Perron, C. et al. (1985) Gene 33, 103–19.
0286VC-W

pGEM®-7Zf(+) Vector GenBank® Accession Number X65310 and vector sequence text file.

Especificaciones

Contenido

Item Part # Presentación

pGEM®-7Zf(+) Vector

P225A 1 × 20μg

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Condiciones de Almacenaje

BB

See Protocol for storage recommendations.

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