ApaI

Cut Site:

G GGCC^▼C
C_▲CCGG G

Note: Sensitive to overlapping dcm methylation.

Incubation Conditions: Buffer A. 37°C.

Source: Acetobacter pasteurianus.

Storage Buffer: 10mM Tris-HCl (pH 7.4), 300mM NaCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.

Percent Activity in 4-CORE® Buffer:

A	B	C	D	MULTI-CORE™
100%	50–75%	50–75%	<10%	75–100%

Frequency of Cutting:

λ	Ad-2	ΦX174	pUC18	M13mp18	pBR322
1	12	0	0	0	0

Protocolos

Complete Protocol

Restriction Enzyme ProtocolPDF (986 KB) – English

Contenido

Item	Part #	Presentación	Concentración	Available Separately
Buffer A 10X Buffer	R001A	1 × 1ml
Bovine Serum Albumin, Acetylated	R396E	1 × 750μl	10mg/ml	View Product
ApaI	R636A	1 × 5,000u
MULTI-CORE™ 10X Buffer	R999A	1 × 0.25ml		View Product

SDS

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Certificado de Análisis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Condiciones de Almacenaje

Recursos

Otros

How to Use Restriction Enzymes: A Resource Guide

Usado con frecuencia junto con

LigaFast™ Rapid DNA Ligation System

Efficiently ligates sticky-ended DNA inserts into plasmid vectors in just 5 minutes at room temperature (blunt-ended inserts in as little as 15 minutes).

M8221, M8225

Blue/Orange Loading Dye, 6X

Colorante para cargar muestras de ADN en pocillos de geles de electroforesis y seguir la migración durante esta.

G1881

Agarose, LE, Analytical Grade

Reactivo para la separación electroforética de ácidos nucleicos.

V3121, V3125

Ethidium Bromide Solution, Molecular Grade

Colorante fluorescente adecuado para la tinción de ácidos nucleicos tras electroforesis o en gradientes de cloruro de cesio.

H5041

λ DNA/EcoRI + HindIII Markers

Trece fragmentos que van desde 125-21.226pb, generados a partir de la digestión de ADN Lambda con EcoRI e HindIII.

G1731