BamHI

Cut Site:

G^▼GATC  C
C   CTAG_▲G

Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/custom/

Usage Notes:

1. Star activity may be observed with glycerol concentrations >12%, enzyme:DNA ratio >25u/μg, low salt conditions or in the presence of Mn²⁺.
2. Ends generated with BamHI can be directly ligated to ends generated with BglII, BclI and XhoII. These restriction sites are not regenerated in the ligation product.

Incubation Conditions: Buffer E. 37°C.

Source: Bacillus amyloliquefaciens H.

Storage Buffer: 10mM Tris-HCl (pH 7.4), 300mM KCl, 5mM MgCl₂, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.

Percent Activity in 4-CORE® Buffer:

*Buffer A is not recommended due to potential star activity.

Frequency of Cutting: