DpnI

Cut Site:

G^meA^▼TC
CT_▲^meAG

Usage Note: DpnI requires N⁶-methylation of the adenine residue for activity, unlike its isoschizomers, MboI and Sau3AI.

Incubation Conditions: Buffer B. 37°C.

Source: Diplococcus pneumoniae.

Storage Buffer: 10mM Tris-HCl (pH 7.4), 50mM NaCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.

Percent Activity in 4-CORE® Buffer:

A	B	C	D	MULTI-CORE™
50–75%	100%	75–100%	50–75%	100%

Frequency of Cutting:

λ	Ad-2	ΦX174	pUC18	M13mp18	pBR322
116	87	0	15	7	22

Protocolos

Complete Protocol

Restriction Enzyme ProtocolPDF (986 KB) – English

Contenido

Item	Part #	Presentación	Concentración	Available Separately
Buffer B 10X Buffer	R002A	1 × 1ml
Bovine Serum Albumin, Acetylated	R396D	1 × 150μl	10mg/ml	View Product
DpnI	R623A	1 × 200u
MULTI-CORE™ 10X Buffer	R999A	1 × 0.25ml		View Product

SDS

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Certificado de Análisis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Condiciones de Almacenaje

Recursos

Otros

How to Use Restriction Enzymes: A Resource Guide

Usado con frecuencia junto con

Ethidium Bromide Solution, Molecular Grade

Colorante fluorescente adecuado para la tinción de ácidos nucleicos tras electroforesis o en gradientes de cloruro de cesio.

H5041

Wizard® Genomic DNA Purification Kit

Método manual basado en soluciones, para extraer ADN a partir de muchos tipos de muestras.

A1120, A1125, A1620