MluI

Cut Site:

A^▼CGCG T
T GCGC_▲A

Usage Note: Ends generated using MluI can be directly ligated to ends generated by BssHII. Neither restriction site is regenerated in the ligation product.

Incubation Conditions: Buffer D. 37°C.

Source: Micrococcus luteus.

Storage Buffer: 10mM Tris-HCl (pH 7.4), 50mM NaCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.

Percent Activity in 4-CORE® Buffer:

A	B	C	D	MULTI-CORE™
10–25%	25–50%	50–75%	100%	10–25%

Frequency of Cutting:

λ	Ad-2	ΦX174	pUC18	M13mp18	pBR322
7	5	2	0	0	0

Protocolos

Complete Protocol

Restriction Enzyme ProtocolPDF (986 KB) – English

Contenido

Item	Part #	Presentación	Concentración	Available Separately
Buffer D 10X Buffer	R004A	1 × 1ml
Bovine Serum Albumin, Acetylated	R396D	1 × 150μl	10mg/ml	View Product
MluI	R638A	1 × 1,000u

SDS

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Certificado de Análisis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Condiciones de Almacenaje

Store at –20°C.

Recursos

Menciones

The conserved active site asparagine in class I ribonucleotide reductase is essential for catalysis.
--
2002 J. Biol. Chem.
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Otros

How to Use Restriction Enzymes: A Resource Guide

Usado con frecuencia junto con

LigaFast™ Rapid DNA Ligation System

Efficiently ligates sticky-ended DNA inserts into plasmid vectors in just 5 minutes at room temperature (blunt-ended inserts in as little as 15 minutes).

M8221, M8225

Blue/Orange Loading Dye, 6X

Colorante para cargar muestras de ADN en pocillos de geles de electroforesis y seguir la migración durante esta.

G1881

Agarose, LE, Analytical Grade

Reactivo para la separación electroforética de ácidos nucleicos.

V3121, V3125

Ethidium Bromide Solution, Molecular Grade

Colorante fluorescente adecuado para la tinción de ácidos nucleicos tras electroforesis o en gradientes de cloruro de cesio.

H5041