Cut Site:
CA▼TA TG
GT AT▲AC
Notes:
- Star activity may be observed with glycerol concentrations >10%.
- For site-specific methylation information, consult McClelland, M., Nelson, M. and Raschke, E. (1994) Nucl. Acids Res. 22, 3640–59.
- Ends generated with NdeI can be directly ligated to ends generated by VspI. Neither recognition site is regenerated in the ligation product.
Incubation Conditions: Buffer D. 37°C.
Source: Neisseria denitrificans.
Storage Buffer: 10mM Tris-HCl (pH 7.4), 300mM NaCl, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.