SgfI

Cut Site:

GCG AT^▼CGC
CGC_▲TA GCG

Notes:

Star activity may be observed with glycerol concentrations >12% or with enzyme:DNA ratios >25u/μg.
Sensitive to CpG methylation.
SgfI is inactivated by freezing at –70°C.
Ends generated by SgfI can be directly ligated to ends generated by PvuI. The PvuI site is regenerated in the ligation product, but the SgfI site is not.

Incubation Conditions: Buffer C. 37°C.

Source: Streptomyces fulvissimis.

Storage Buffer: 10mM Tris-HCl (pH 7.4), 300mM KCl, 0.1% Thesit®, 0.1mM EDTA, 1mM DTT, 0.5mg/ml BSA, 50% glycerol.

Percent Activity in 4-CORE® Buffer:

A	B	C	D	MULTI-CORE™
25–50%	25–50%	100%	<10%	<10%

Frequency of Cutting:

λ	Ad-2	ΦX174	pUC18	M13mp18	pBR322
0	1	0	0	0	0

Protocolos

Complete Protocol

Restriction Enzyme ProtocolPDF (986 KB) – English

Contenido

Item	Part #	Presentación	Concentración	Available Separately
Buffer C 10X Buffer	R003A	1 × 1ml
Bovine Serum Albumin, Acetylated	R396D	1 × 150μl	10mg/ml	View Product
SgfI	R710B	1 × 250u

SDS

Search for SDS

Certificado de Análisis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Condiciones de Almacenaje

Recursos

Otros

How to Use Restriction Enzymes: A Resource Guide

Productos Similares

PvuI

Enzima de restricción que corta ADN a 37°C y deja un extremo 3'.

R6321, R6325

Usado con frecuencia junto con

LigaFast™ Rapid DNA Ligation System

Efficiently ligates sticky-ended DNA inserts into plasmid vectors in just 5 minutes at room temperature (blunt-ended inserts in as little as 15 minutes).

M8221, M8225

Blue/Orange Loading Dye, 6X

Colorante para cargar muestras de ADN en pocillos de geles de electroforesis y seguir la migración durante esta.

G1881

Agarose, LE, Analytical Grade

Reactivo para la separación electroforética de ácidos nucleicos.

V3121, V3125

Ethidium Bromide Solution, Molecular Grade

Colorante fluorescente adecuado para la tinción de ácidos nucleicos tras electroforesis o en gradientes de cloruro de cesio.

H5041