The BHB-Glo™ (Ketone Body) Assay is a fast and sensitive method for detecting β-hydroxybutyrate (BHB) from various biological samples, such as cultured cells, media, tissue homogenates and serum. Based on bioluminescent technology, the BHB-Glo™ Assay can detect subtle changes in ketosis as well as ketone body consumption and secretion. Its streamlined sample preparation protocol involves acid treatment and neutralization directly in the assay wells, eliminating the need for cell collection, centrifugation, spin columns and deproteinization. The assay is adaptable to a 384-well format, facilitating quick analysis of many samples.