Glucose-Glo™ Assay

Rapid, Selective and Sensitive Glucose Detection in Biological Samples

The Glucose-Glo™ Assay is a bioluminescent assay for rapid and sensitive measurement of glucose from a variety of sample types. The bioluminescent signal eliminates signal interference that colorimetric and fluorescent glucose assays suffer, and there is no need for deproteinization sample preparation steps. The Glucose-Glo™ Assay is suitable for detecting altered glucose consumption due to changes in glycolysis or glucose production during gluconeogenesis.

How the Assay Works

The Glucose-Glo™ Assay couples glucose oxidation and NADH production with a bioluminescent NADH detection system. Glucose dehydrogenase uses glucose and NAD+ to produce NADH. In the presence of NADH a pro-luciferin Reductase Substrate is converted by Reductase to luciferin that is then used by Ultra-Glo™ Recombinant Luciferase to produce light.

When Glucose Detection Reagent, which contains glucose dehydrogenase (GDH), NAD+, Reductase, Reductase Substrate and Luciferase, is added to a sample containing glucose at a 1:1 ratio, the enzyme-coupled reactions start and run simultaneously. The luminescent signal is proportional to the amount of glucose in the sample and increases until all glucose is consumed, at which time a stable luminescent signal is achieved.

13887ma-w: Luminescence from glucose assay proportional to metabolite — Glucose titration curve. Serial dilutions of glucose were prepared and assay performed as described in TM494.

Amenable to Your Throughput and Workflow Requirements

The Glucose-Glo™ Assay is a versatile detection kit that is amenable to high-throughput formats and compatible with many sample types including cells, lysates, tissue, plasma and serum. To simplify sample processing, the glucose detection kit uses processing methods that do not require sample centrifugation or spin columns. Simply dilute your samples, add inactivation and neutralization solutions (if needed), and assay all samples directly in 96- or 384-well plates.

13888ma-w: Luminescent glucose assay measured consumption for 72 hours — A549 cells were plated at 5,000 (light blue bars) and 15,000 (dark blue bars) cells/well in medium. Wells with medium only were included as controls. At indicated time points, 2.5µl of medium was removed and diluted. Samples were stored as indicated before assaying as described in TM494. Red line represents luminescence values of medium-only controls.

Measure Glycolysis and Glutaminolysis from One Sample

Easily measure changes in cellular glycolysis and glutaminolysis by sampling medium over time. Direct measurement of four metabolites important to the energetic state of the cell—glucose, lactate, glutamate and glutamine—can be performed in parallel using the bioluminescent Glucose-Glo™, Lactate-Glo™, Glutamine/Glutamate-Glo™ and Glutamate-Glo™ Assays. No specialized instrument, plates or artificial medium is required. Sample processing compatible with all of the bioluminescent metabolite assays means the same sample can be used for detecting all four metabolites. This includes sample types such as culture medium, serum, plasma and tissue. The data below were produced by measuring metabolites using medium samples from the same cell cultures as described in Technical Manual TM494.

13836ma-w-a: Glucose consumption measured in metabolite multiplex assay.

13836ma-w-b: Lactate secretion measured in metabolite multiplex assay.

13836ma-w-c: Glutamine consumption measured in metabolite multiplex assay.

13836ma-w-d: Glutamate secretion measured in metabolite multiplex assay.

Advantages for Glucose-Glo™ Assay

Quickly Detect Glucose in a Variety of Sample Types: Measure in medium, cells, tissue and plasma. The glucose detection assay requires minimal preparation steps with no need for centrifugation and spin columns.

Detect Small Changes in Glucose Levels Across a Wide Range of Concentrations: Sensitive glucose assay with broad linearity better discriminates small changes in glucose compared to colorimetric and fluorometric glucose assays.

Gain More Information Per Sample: The glucose detection assay along with the other bioluminescent metabolite detection assays are amenable to measuring multiple metabolites from the same sample. Multiplex with cell viability assays for normalization.

Requires Only a Luminometer: The same plate reader that measures cell viability can be used to monitor glycolysis. No specialized instruments, plates or artificial medium are required.

Protocolos

Complete Protocol

Glucose-Glo™ Assay Technical ManualPDF (1204 KB) – English

Contenido

Item	Part #	Presentación	Concentración
Reductase	G884A	1 × 55μl	6mg/ml
Reductase Substrate	G885A	1 × 55μl
Luciferin Detection Solution	J135A	1 × 5ml
NAD	J136A	1 × 30μl
Glucose	J138A	1 × 50μl
Glucose Dehydrogenase	J140A	1 × 200μl

SDS

Search for SDS

Certificado de Análisis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Condiciones de Almacenaje

Patentes y Exclusiones

U.S. Pat. Nos. 9,273,343 and 9,951,372, European Pat. No. 2751089, Japanese Pat. No. 6067019 and other patents pending.

Contenido

Item	Part #	Presentación	Concentración
Reductase	G884B	1 × 275μl	6mg/ml
Reductase Substrate	G885B	1 × 275μl
Luciferin Detection Solution	J135B	1 × 50ml
NAD	J136B	1 × 275μl
Glucose	J138A	1 × 50μl
Glucose Dehydrogenase	J140B	2 × 1ml

SDS

Search for SDS

Certificado de Análisis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Condiciones de Almacenaje

Patentes y Exclusiones

U.S. Pat. Nos. 9,273,343 and 9,951,372, European Pat. No. 2751089, Japanese Pat. No. 6067019 and other patents pending.

Recursos

Menciones

Posters

Otros

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Detect Even Small Changes in Glucose

Rapid, Selective and Sensitive Glucose Detection in Biological Samples

How the Assay Works

Luminescent Signal is Proportional to Amount of Glucose Present

Amenable to Your Throughput and Workflow Requirements

Glucose Consumption by Adherent Lung Carcinoma A549 Cells

Measure Glycolysis and Glutaminolysis from One Sample

Advantages for Glucose-Glo™ Assay

Further Reading About Glucose-Glo™ Assay Development

Protocolos

Complete Protocol

Especificaciones

Contenido

SDS

Certificado de Análisis

Use Restrictions

Condiciones de Almacenaje

Patentes y Exclusiones

Especificaciones

Contenido

SDS

Certificado de Análisis

Use Restrictions

Condiciones de Almacenaje

Patentes y Exclusiones

Recursos

Menciones

Posters

Otros

Glucose-Glo™ Assay

Detect Even Small Changes in Glucose

Rapid, Selective and Sensitive Glucose Detection in Biological Samples

How the Assay Works

Luminescent Signal is Proportional to Amount of Glucose Present

Amenable to Your Throughput and Workflow Requirements

Glucose Consumption by Adherent Lung Carcinoma A549 Cells

Measure Glycolysis and Glutaminolysis from One Sample

Advantages for Glucose-Glo™ Assay

Further Reading About Glucose-Glo™ Assay Development

Protocolos

Complete Protocol

Especificaciones

Contenido

SDS

Certificado de Análisis

Use Restrictions

Condiciones de Almacenaje

Patentes y Exclusiones

Especificaciones

Contenido

SDS

Certificado de Análisis

Use Restrictions

Condiciones de Almacenaje

Patentes y Exclusiones

Recursos

Menciones

Posters

Otros

Productos Similares

Lactate-Glo™ Assay

Glycogen-Glo™ Assay

Lumit® Glucagon Immunoassay

Lumit® Immunoassays para la detección de reguladores metabólicos

Usado con frecuencia junto con

CellTiter-Glo® 2.0 Cell Viability Assay

CellTiter-Glo® One Solution Assay

RealTime-Glo™ MT Cell Viability Assay

GloMax® Discover System

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