The Metabolite-Glo™ Detection System is a rapid and sensitive approach for detecting NAD(P)H in a variety of biological samples, including cultured cells, media samples, tissue homogenates and serum. This system can be optimized to detect specific metabolites by coupling NAD(P)H detection with a suitable dehydrogenase enzyme (which must be obtained separately). We provide straightforward instructions for metabolite assay development and optimization in the technical manual.
The Metabolite-Glo™ System provides reagents for a streamlined sample preparation with acid treatment and neutralization steps performed directly in the assay wells. This eliminates the need for traditional methods such as cell collection, centrifugation and spin columns. With its sensitive detection capabilities and liquid formulation, the assay is easily adapted to a high-throughput 384-well format, enabling the efficient analysis of numerous samples simultaneously.