NAD(P)H-Glo™ Detection System

G9061_NADPH-Glo Detection System_3
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Monitor the Concentration of Reduced Forms of NADH and NADPH

  • Highly sensitive and rapid bioluminescent assay
  • Easily adapted for high-throughput format
  • Simple add-mix-measure protocol

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Catalog number selected: G9061

$ 447.00
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NAD(P)H-Glo™ Detection System
10ml
$ 447.00
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11738MD-W

The NAD(P)H-Glo™ Detection System is a bioluminescent, homogeneous, single-reagent-addition assay that quantitatively monitors the concentration of the reduced forms of NADH and NADPH levels in enzymatic reactions. The oxidized forms, NAD+ and NADP+, are not detected and do not interfere with quantitation.

The assay is rapid, requiring only a 40- to 60-minute incubation, has a broad linear range (25nM-50µM) and high signal-to background ratio (~400). Low nanomolar sensitivity, 25nM, allows direct in-well detection. The assay is well suited to measuring NAD(P)H production or consumption in high-throughput formats.

Assay Chemistry

In the presence of NAD(P)H, a reductase reduces the proluciferin reductase substrate to form luciferin. Luciferin is quantified using Ultra-Glo™ Recombinant Luciferase, which is a component of the Luciferin Detection Reagent, and the light signal produced is proportional to the amount of NAD(P)H in the sample.

NADP_NADH_Foreground-Image

Sensitive and Selective

The assay is selective for the reduced forms of NADH and NADPH levels in enzymatic reactions.

11945MB-W

Compatible with High-Throughput Systems

The simple add-and-read protocols are compatible with automated and high-throughput workflow, and the flexible chemistries adapt to 96-, 384- and 1,536-well plate formats and high-throughput systems. They yield Z′ values >0.7.

Assays were performed using 8µl of the indicated concentration of NADH with 8µl of the appropriate Luciferin Detection Reagent in 384-well plates. S/B = signal-to-background ratio.

11580MC-W

The NAD(P)H-Glo™ Assay detects only NADH and NADPH.


Case Study

Researchers at BioAscent have implemented the NAD(P)H-Glo™ Assay for HTS. In this project, Dr McElroy and his team used the NAD(P)H-Glo™ Assay to screen 50,000 compounds against a poorly characterized enzyme that is a potential drug target. NAD(P)H-Glo was chosen as it has high sensitivity, with a wide dynamic range and high signal-to-background ratios.

Download Case Study (PDF)

Especificaciones

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Contenido

Item Part # Presentación Concentración

Reductase

G884A 1 × 55μl 6mg/ml

Reductase Substrate

G885A 1 × 55μl

Luciferin Detection Reagent

V859A 1 × 1 each

Reconstitution Buffer

V865A 1 × 10ml

Certificado de Análisis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Condiciones de Almacenaje

AA

Patentes y Exclusiones

Patent Pending.

U.S. Pat. Nos. 9,273,343 and 9,951,372, European Pat. No. 2751089, Japanese Pat. No. 6067019 and other patents pending.

Especificaciones

You are viewing: G9062 Cambiar Configuración

Contenido

Item Part # Presentación Concentración

Reductase

G884B 1 × 275μl 6mg/ml

Reductase Substrate

G885B 1 × 275μl

Luciferin Detection Reagent

V859B 1 × 1 each

Reconstitution Buffer

V865B 1 × 50ml

Certificado de Análisis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Condiciones de Almacenaje

AA

Patentes y Exclusiones

Patent Pending.

U.S. Pat. Nos. 9,273,343 and 9,951,372, European Pat. No. 2751089, Japanese Pat. No. 6067019 and other patents pending.

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