MethylEdge® Bisulfite Conversion System

Rapid Bisulfite Conversion with Minimal DNA Fragmentation

  • High-efficiency DNA conversion from as little as 100pg input DNA
  • Rapid protocol saves time compared to other conversion systems
  • Robust conversion of DNA with reduced DNA fragmentation
customize-this-small
View information on global supply logistics

Presentación

Catalog number selected: N1301

$ 368.00
Your price:
Add to Cart
This product is discontinued
Add to Helix
This product can be added to Helix

This product is available through the Promega Helix onsite stocking program. We offer numerous convenient solutions to meet your lab's needs.

Learn more about the Helix Collection »

This product is available under our Early Access program - Learn More
This product is available under our Catalog (FT) program - Learn More
MethylEdge® Bisulfite Conversion System
50 reactions
$ 368.00
Your price: Acceder

Bisulfite Conversion for DNA Methylation Workflows

The MethylEdge® Bisulfite Conversion System provides a rapid, efficient method to perform bisulfite conversion with minimal DNA fragmentation in less than two hours. The rapid protocol and complete conversion mean that you can produce completely converted DNA ready for your choice downstream assays with minimal preparation and hands-on time. Coupled with purification chemistries and flexible, robust amplification technologies for detection, the MethylEdge® Bisulfite Conversion System delivers high-quality, intact converted DNA for your experiments.

  • High-efficiency DNA conversion
  • Robust conversion of DNA with reduced DNA fragmentation
  • Rapid Protocol
  • Room-Temperature, Ready-to-Use Reagents

This system can be used for the bisulfite conversion and cleanup of DNA. Applications for converted DNA include bisulfite-specific PCR (BSP), bisulfite sequencing (bis-seq), bisulfite-specific high-resolution melt and COBRA analysis.

32463455-3D-Histones2

Rapid Conversion, Minimal Fragmentation

Realize the full potential of your samples with the most effective bisulfite conversion system available. The MethylEdge™ Bisulfite Conversion System offers a robust system for rapidly performing bisulfite conversion with minimal DNA fragmentation.

  • High-efficiency DNA conversion from as little as 100pg input DNA 
  • Rapid Protocol saves time compared to other conversion systems
  • Robust conversion of DNA with reduced DNA fragmentation
  • Ready-to-use reagents stored at room temperature
11407MA-W
Completely convert your DNA in less than 2 hours and go directly into your downstream assay of choice. This all-inclusive kit provides you with more intact, usable, DNA for any application including qPCR, arrays, and sequencing.

Degree of DNA Fragmentation After Conversion

11325TA-W
DNA was purified from whole blood using the ReliaPrep™ Blood gDNA Miniprep System (1) or Maxwell® 16 LEV Blood Kit (2) and was converted using the MethylEdge® Bisulfite Conversion System (P) or a competing bisulfite conversion kit (Z or N). B = BenchTop 1kb DNA Ladder, D = nonconverted, purified DNA.

DNA Purification and Quantitation

The success of DNA methylation analysis experiments relies on quality genomic DNA as input. We offer high-quality systems for manual and automated genomic DNA purification from many sample types.
Accurate DNA quantitation of both pre- and post-bisulfite conversion is critical to experimental success. We offer robust, fluorescence-based detection systems for accurate quantitation.

Manual and High-Throughput Chemistries

ReliaPrep™ Genomic DNA Purification Systems offer comprehensive solutions for reliable purification of high-quality gDNA. Kits include manual systems for purification from FFPE samples, blood and tissue and high-throughput gDNA purification from large volume blood samples.

Hands-Free Automated Purification from 1–16 Samples

The Maxwell® RSC Instrument and DNA Purification Kits provide automated DNA extraction from many sample types, including FFPE tissues. The convenient, hands-free extraction method frees up time and provides consistent yields of highly pure gDNA.

Quantitation Instrument

Quantus™ Fluorometer

11767TA

Chemistry

The QuantiFluor™ dsDNA System offers sensitive detection of small amounts of double stranded DNA in solution upstream of bisulfite conversion.

The QuantiFluor™ ssDNA System offers reliable, sensitive detection of bisulfite converted DNA in solution.

Downstream Analysis

Amplify your bisulfite-converted DNA with the most robust, reliable amplification tools on the market. Whether your methylation assay requires real-time or end-point analysis, the GoTaq® Amplification Systems provide easy-to-use, sensitive reagents that meet your needs.
End-Point PCR Systems Dye-Based Real-Time Systems Probe-Based Real-Time Systems

GoTaq® Hot Start Master Mixes are premixed, ready-to-use solutions containing GoTaq® Hot Start Polymerase, magnesium, dNTPs and buffer. Reactions can be set up in less than a minute at room temperature; simply add your template, water and primers.

GoTaq® G2 Hot Start Master Mixes

GoTaq® qPCR Master Mix provides robust real-time PCR with earlier quantification cycle values and broad range detection for increased reliability, reproducibility and sensitivity. The system contains BRYT Green® dye, a novel fluorescent DNA-binding dye with minimal PCR inhibition for maximum efficiency, and greater fluorescence enhancement upon binding to double-stranded DNA than SYBR® Green I.

GoTaq® qPCR Master Mix

The GoTaq® Probe qPCR Master Mix is optimized for quantitative PCR assays in the hydrolysis probe detection format. It is provided as a ready-to-use, stabilized 2X formulation that includes all components for qPCR (except template, primers and probe).

GoTaq® Probe qPCR Master Mix

Protocolos

Complete Protocol

Protocolos Rápidos

Especificaciones

Contenido

Item Part # Presentación

Collection Tubes

 N124A 1 × 50/pack

ME Spin Columns

 N125A 1 × 50/pack

ME Elution Buffer

 N126A 1 × 1ml

ME Desulphonation Buffer

 N127A 1 × 10ml

ME Wash Buffer

 N128A 1 × 6ml

ME Binding Buffer

 N129A 1 × 30ml

ME Conversion Reagent

 N130A 5 × 1.3ml

SDS

Search for SDS

Certificado de Análisis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Condiciones de Almacenaje

DD

Store the MethylEdge® Bisulfite Conversion System at 22–25°C (room temperature). Store the Methylated Human Control at 2–10°C. Store the Converted Methylated Human Control at –30 to –10°C.

Patentes y Exclusiones

Use of Methylation-Specific PCR (MSP) is protected by U.S. Pat. Nos. 5,786,146, 6,017,704, 6,200,756 and 6,265,171. No license under these patents to use the MSP process is conveyed expressly or by implication to the purchaser by the purchase of this product.

Recursos

Menciones

Productos Similares

MTase-Glo™ Methyltransferase Assay

Measure methyltransferase activity using a universal bioluminescent assay.

V7601, V7602

Usado con frecuencia junto con

ReliaPrep™ Blood gDNA Miniprep System

Purificación rápida y manual de ADN de alta calidad a partir de muestras de sangre o fluidos corporales de hasta 200μl.

A5081, A5082

ReliaPrep™ FFPE gDNA Miniprep System

Purificación rápida y manual de ADN a partir de tejidos FFPE, sin utilizar disolventes orgánicos.

A2351, A2352

QuantiFluor® dsDNA System

Sistema de cuantificación sensible y específico de ADN de doble hélice.

E2670, E2671

Encontremos el producto que se adapte a sus necesidades.

Hablar con un científico

Jan

Jan

Germany
Choose your country

Americas

Brazil
/
Canada
/
United States
/

Pacific Asia

Australia
China
/
India
Japan
/
Korea, Republic of
/
Singapore

Europe

Austria
/
Belgium
Denmark
(Incl. Faroe Isl. & Greenland)
Estonia
Finland
(Incl. Åland)
France
/
Germany
/
Iceland
Ireland
Italy
/
Luxembourg
Netherlands
Norway
(Incl. Svalbard)
Poland
/
Spain
/
Sweden
Switzerland
United Kingdom

Gestionar cookies

Cookies estrictamente necesarias

El uso de estas cookies asegura la funcionalidad del sitio web; son necesarias para la prestación de nuestros servicios y no pueden ser desactivadas en nuestro sistema. Únicamente se activan bajo petición expresa del usuario cuando inicia sesión, usa el carrito de la compra o rellena un formulario. Puede configurar su navegador para bloquear o avisarle sobre este tipo de cookies, pero algunas funcionalidades de nuestro servicio no funcionarán correctamente sin estas cookies. Como sucede con el resto de cookies, parte pueden ser propias y parte de terceros.

Cookies de sesión

Empleamos estas cookies para recordar sus preferencias de navegación. Por ejemplo, para recordar sus preferencias sobre el idioma de la página web.

Cookies de rendimiento

Utilizamos cookies para analizar la forma en la que los usuarios interactúan con nuestros servicios, para evaluar y mejorar la experiencia de navegación. Por ejemplo, podemos emplear esas cookies para contar las visitas a una determinada página web.

Cookies de marketing

Nosotros y nuestros proveedores publicitarios usamos estas cookies para el envío de anuncios, haciéndolos más relevantes e interesantes para usted en función de sus preferencias y para medir la efectividad de nuestras campañas publicitarias en nuestra web, en otros medios de comunicación digitales y/o en redes sociales.

Your Privacy Choices

Our website supports your privacy choices by allowing users in select regions to opt out of specific types of sharing/sales/use of cookies and technologies for Targeted Advertising. Visit our Privacy Policy and Cookie Policy pages to learn more about these topics.

Targeted Advertising

Deselect this checkbox to disable cookies related to targeted advertising in your Promega experience. These advertising cookies will no longer be set for this browser and device.

Note: These settings are based on necessary cookies that are placed on your device, and if those cookies are cleared or you access the website from another browser or device, advertising cookies might be reset by default in the future, from which you can opt-out.