PowerQuant® System

Accurate and Sensitive DNA Quantification on the First Try

The PowerQuant® System is a 5-color, 4-target probe-based qPCR assay that quantifies the total amount of amplifiable autosomal and Y-chromosomal DNA in a single assay using the same DNA standards. This system provides flexible options for 4-point to 7-point standard curve. The kit contains an internal PCR control (IPC) to test for false-negative results that may occur in the presence of PCR inhibitors.

Trust your zero quantification results—zero means zero
Obtain reliable quantification results with more consistent auto/Y ratios
Maximize your success in STR assays with straightforward DNA quality assessment
Shorten time to results with a faster cycling time of approximately 1 hour
New software allows sample name import, data analysis and customized visualization

The PowerQuant® System is optimized for use on the following instruments:

Applied Biosystems® 7500 Real-Time PCR System (Applied Biosystems® 7500 Software, Version 2.0.6)
Applied Biosystems® 7500 Real-Time PCR System for Human Identification (HID Real-Time PCR Analysis, Version 1.1 or 1.2)
Applied Biosystems® QuantStudio™ 5 Real-Time PCR System (Design and Analysis Desktop Software, Versions 1.5 and 1.5.1)

Learn more about how the Casework Direct System and the PowerQuant® System can help improve processing efficiency for sexual assault evidence kits (SAEK).

Promega forensic products are manufactured in alignment with the ISO 18385 standard. This standard ensures minimal risk of human DNA contamination for products used to collect, store and analyze biological materials for forensic purposes. Learn more.

Use DNA Quantification Results to Reliably Predict STR Results

The PowerQuant® System can detect DNA at concentrations below which an informative profile can be obtained, even when the maximum sample volume is used in an STR amplification reaction.

A DNA sample was serially diluted to low template concentrations and quantified in quadruplicate using the PowerQuant® System. Data in the PowerQuant® System table indicate the number of replicates in which the amplification curve crossed the amplification threshold within 39 cycles divided by the total number of replicates tested. Auto = autosomal target, Y = male target, D = degradation target. These samples were also amplified in quadruplicate using the PowerPlex® Fusion System by adding the maximum input volume (15μl) per 25μl amplification reaction (except a template amount of 500ng was added for the 100pg/μl dilution). Data in the PowerPlex® Fusion System table show the average number of alleles detected in the STR reaction when analyzed using an Applied Biosystems® 3130 Genetic Analyzer and a 50RFU threshold.

Representative PowerPlex® Fusion data obtained with 500pg, 150pg, 15pg and 1.5pg of DNA template. — Representative PowerPlex® Fusion data obtained with DNA template. Casework samples often contain DNA mixtures from multiple persons. The Y-chromosomal target in the PowerQuant® System enables quantification of human male DNA in the sample, helping you evaluate mixtures of male and female DNA. The PowerQuant® System also allows you to make better decisions when faced with a partial or failed STR amplification by differentiating between PCR inhibition and DNA degradation.

Reliably Detect PCR Inhibitors

Reliable detection of inhibitors that can affect DNA quantification and downstream STR assays is a key feature of the PowerQuant® System. The internal PCR control (IPC) has roughly the same level of sensitivity to inhibitors as currently available downstream STR assays, allowing you to determine if a sample is appropriate for amplification or if additional processing is required.

IPC C_q shift predicts downstream STR inhibition

In contrast to the sample with the lower humic acid concentration, the IPC C_q shifts for the samples with higher humic acid (HA) concentration (400ng/µl) indicate the presence of an inhibitor and concomitantly show a significant reduction in STR peak heights in the PowerPlex® Fusion System electropherograms. In this example, the [Auto]/[D] ratios are not affected at these humic acid concentrations. This illustrates that the IPC is more sensitive to inhibitors than the degradation target, enabling distinction between sample inhibition and degradation.

Assess Integrity of Your DNA Sample

The system amplifies a short autosomal and a long autosomal target. After amplification, the PowerQuant® Analysis Software calculates the ratio of DNA concentrations for the shorter and longer amplicons. The resulting degradation ratio helps you determine if a DNA sample is degraded, rather than inhibited, and decide which, if any, STR system is most appropriate for downstream analysis. The experiment below illustrates that the [Auto]/[D] ratio is reflective of sample integrity.

DNA samples exposed to increasing amounts of UV radiation on the 7500 and QuantStudio 5 Systems — Comparison of [Auto]/[Deg] values obtained from PowerQuant® quantification of degraded DNA sample on the Applied Biosystems® 7500 and QuantStudio™ 5 Real-Time qPCR Systems. Samples were exposed to increasing amounts of UV radiation.

Want to Automate?

Save time and improve precision by automating your DNA quantification setup. The PowerQuant® and Plexor® HY Systems can be automated on the Biomek® 4000 (Beckman Coulter), Freedom EVO (Tecan), or MicroLab STAR (Hamilton) liquid handlers. Additionally, import the quantification data into the STR Normalization Manager Software to automate your PCR amplifications setup.

Automate your DNA Quantification Setup

Contact your local representative to receive a quote for the required quantities and to purchase and schedule the required installation and training services.

Hardware

Product	Cat.#
Four-Position Tube Holder	V1601
Plate Clamp 96	V8251
Plate Stand	V8261

Protocolos

Complete Protocol

PowerQuant® System Technical ManualPDF (20939 KB) – English

Protocolos Rápidos

PowerQuant® System Quick Protocol FB186PDF (95 KB) – English

PowerQuant® Analysis Software Quick Start Guide FB241PDF (1867 KB) – English

Contenido

Item	Part #	Presentación	Available Separately
PowerQuant® Male gDNA Standard	DD302A	1 × 150μl	View Product
PowerQuant® 20X Primer/Probe/IPC Mix	DK930A	1 × 220μl
PowerQuant® 2X Master Mix	DP527A	2 × 1.1ml
Water, Amplification Grade	DW099A	2 × 1,250μl	View Product
PowerQuant® Dilution Buffer	Y101A	2 × 1.5ml

SDS

Search for SDS

Certificado de Análisis

Search by lot number

Use Restrictions

Not For Medical Diagnostic Use.

Condiciones de Almacenaje

Patentes y Exclusiones

Dye compounds in this product are sold under license from Biosearch Technologies, Inc., and protected by U.S. and worldwide patents either issued or in application. The license does not include rights for human IVD use.

Contenido

Item	Part #	Presentación	Available Separately
PowerQuant® Male gDNA Standard	DD302A	3 × 150μl	View Product
PowerQuant® 20X Primer/Probe/IPC Mix	DK930A	4 × 220μl
PowerQuant® 2X Master Mix	DP527A	8 × 1.1ml
Water, Amplification Grade	DW099A	5 × 1,250μl	View Product
PowerQuant® Dilution Buffer	Y101A	8 × 1.5ml

SDS

Search for SDS

Certificado de Análisis

Search by lot number

Use Restrictions

Not For Medical Diagnostic Use.

Condiciones de Almacenaje

Patentes y Exclusiones

Dye compounds in this product are sold under license from Biosearch Technologies, Inc., and protected by U.S. and worldwide patents either issued or in application. The license does not include rights for human IVD use.

Contenido

Item	Part #	Presentación
PowerQuant® Calibration Standard, FAM	DS106A	1 × 60μl
PowerQuant® Calibration Standard, CFG540	DS107A	1 × 60μl
PowerQuant® Calibration Standard, TMR	DS108A	1 × 60μl
PowerQuant® Calibration Standard, Q670	DS109A	1 × 60μl
PowerQuant® Calibration Standard, CXR	DS110A	1 × 60μl
PowerQuant® Calibration Buffer	DS111A	1 × 30ml

SDS

Search for SDS

Certificado de Análisis

Search by lot number

Use Restrictions

Not For Medical Diagnostic Use.

Condiciones de Almacenaje

Patentes y Exclusiones

Dye compounds in this product are sold under license from Biosearch Technologies, Inc., and protected by U.S. and worldwide patents either issued or in application. The license does not include rights for human IVD use.

Recursos

Artículos

Menciones

Human DNA quantification and sample quality assessment: Developmental validation of the PowerQuant® system.

--
2016 Forensic Sci. Int. Genet.
Ver todas las menciones

Software y Firmware

PowerQuant® Analysis

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PowerQuant® System

5-Color, 4-Target Probe-Based qPCR Assay

Accurate and Sensitive DNA Quantification on the First Try

Use DNA Quantification Results to Reliably Predict STR Results

Reliably Detect PCR Inhibitors

IPC C_q shift predicts downstream STR inhibition

Assess Integrity of Your DNA Sample

Want to Automate?

Automate your DNA Quantification Setup

Protocolos

Complete Protocol

Protocolos Rápidos

Especificaciones

Contenido

SDS

Certificado de Análisis

Use Restrictions

Condiciones de Almacenaje

Patentes y Exclusiones

Especificaciones

Contenido

SDS

Certificado de Análisis

Use Restrictions

Condiciones de Almacenaje

Patentes y Exclusiones

Especificaciones

Contenido

SDS

Certificado de Análisis

Use Restrictions

Condiciones de Almacenaje

Patentes y Exclusiones

Recursos

Artículos

Menciones

Software y Firmware

Otros

PowerQuant® System

5-Color, 4-Target Probe-Based qPCR Assay

Accurate and Sensitive DNA Quantification on the First Try

Use DNA Quantification Results to Reliably Predict STR Results

Reliably Detect PCR Inhibitors

IPC Cq shift predicts downstream STR inhibition

Assess Integrity of Your DNA Sample

Want to Automate?

Automate your DNA Quantification Setup

Protocolos

Complete Protocol

Protocolos Rápidos

Especificaciones

Contenido

SDS

Certificado de Análisis

Use Restrictions

Condiciones de Almacenaje

Patentes y Exclusiones

Especificaciones

Contenido

SDS

Certificado de Análisis

Use Restrictions

Condiciones de Almacenaje

Patentes y Exclusiones

Especificaciones

Contenido

SDS

Certificado de Análisis

Use Restrictions

Condiciones de Almacenaje

Patentes y Exclusiones

Recursos

Artículos

Menciones

Software y Firmware

Otros

Productos Similares

Plexor® HY System

Usado con frecuencia junto con

PowerPlex® Fusion 6C System

PowerPlex® Y23 System

Americas

Pacific Asia

Europe

IPC C_q shift predicts downstream STR inhibition