PowerPlex® 16 HS System

Multiplex STR System for Use in DNA Typing

  • All 16 loci amplified simultaneously in single tube and analyzed in single injection
  • Achieve high discrimination to resolve challenging cases
  • Master mix more tolerant of PCR inhibitors
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Catalog number selected: DC2101

$ 3,490.00
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PowerPlex® 16 HS System
100 reactions
$ 3,490.00
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STR Analysis Using the PowerPlex® 16 HS System

The PowerPlex® 16 HS System is a multiplex STR system for use in DNA typing. This system co-amplifies the loci D18S51, D21S11, TH01, D3S1358, Penta E (labeled with fluorescein); FGA, TPOX, D8S1179, vWA and Amelogenin (labeled with TMR); CSF1PO, D16S539, D7S820, D13S317, D5S818 and Penta D (labeled with JOE). This multiplex includes all 13 CODIS STR markers, Amelogenin for gender determination and two low-stutter, highly discriminating pentanucleotide STR markers. The loci included in the PowerPlex® 16 HS System are more discriminating than competitive systems and are ideal for resolving partial matches or challenging familial cases.

The PowerPlex® 16 HS System is easy to use and includes size standard, amplification-grade water and Taq DNA polymerase already in the master mix. All sixteen loci are amplified simultaneously in a single tube and analyzed in a single injection. This system produces full profiles with 100pg of DNA, giving you confidence in the analysis of precious samples. The PowerPlex® 16 HS System is more tolerant of PCR inhibitors than competing STR systems and the previous version of the PowerPlex® 16 System, increasing your success rate and reducing the need for re-amplification. The PowerPlex® 16 HS System master mix is more tolerant of PCR inhibitors than competitive STR systems.

Panels and bins text files are required for automatic assignment of genotypes using the GeneMapper® ID and ID-X software and are available for download. Primer sequences, dyes and ladders are all unchanged from the PowerPlex® 16 System, providing concordance with existing databases. The PowerPlex® 16 HS System is compatible with the Spectrum Compact CE System and the Applied Biosystems® 3130, 3130xl, 3500 and 3500xL Genetic Analyzers.

Reference

  1. Konjhodic, R. et al. (ICMP) (2004) Comparative study of Penta D and Penta E with D2 and D19, IDENTITY Newsletter 5,1–2.
Sensitivity of the PowerPlex 16 HS System.

Decreasing amounts of template were amplified using the PowerPlex® 16 HS System. Each amplification was performed as directed in the PowerPlex® 16 HS System protocol. Once amplified, samples were separated on an Applied Biosystems 3130 Genetic Analyzer using 3kV, 5-second injection. In each case all expected alleles were called using a 50RFU threshold.

Rapid Processing Solutions for PowerPlex®

The 5X AmpSolution™ Reagent, SwabSolution™ Kit and PunchSolution™ Kit allow fast and simple processing of swabs and punches for analysis with various PowerPlex® Systems, including the PowerPlex® Fusion, 21, 18D, 16 HS, ESX, ESI, CS7 and Y23 Systems. These products are intended for preparation of single-source reference, database and paternity samples where sample purification is unnecessary.

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Especificaciones

You are viewing: DC2101 Cambiar Configuración

Contenido

Item Part # Presentación Concentración Available Separately

2800M Control DNA

DD710A 1 × 25μl 10ng/μl View Product

Internal Lane Standard 600

DG107A 1 × 150μl View Product

PowerPlex® 16 HS Allelic Ladder Mix

DG108B 1 × 50μl

PowerPlex® 16 HS 10X Primer Pair Mix

DK112B 1 × 250μl

PowerPlex® HS 5X Master Mix

DP101A 1 × 500μl

Water, Amplification Grade

DW099A 5 × 1,250μl View Product

Certificado de Análisis

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Use Restrictions

Not For Medical Diagnostic Use.

Condiciones de Almacenaje

BB

Store at –20°C.

Especificaciones

You are viewing: DC2100 Cambiar Configuración

Contenido

Item Part # Presentación Concentración Available Separately

2800M Control DNA

DD710A 1 × 25μl 10ng/μl View Product

Internal Lane Standard 600

DG107A 4 × 150μl View Product

PowerPlex® 16 HS Allelic Ladder Mix

DG108B 4 × 50μl

PowerPlex® 16 HS 10X Primer Pair Mix

DK112B 4 × 250μl

PowerPlex® HS 5X Master Mix

DP101A 4 × 500μl

Water, Amplification Grade

DW099A 10 × 1,250μl View Product

Certificado de Análisis

Search by lot number

Use Restrictions

Not For Medical Diagnostic Use.

Condiciones de Almacenaje

BB

Store at –20°C.

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