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Additional Signaling Pathway Analysis Response Element Firefly Luciferase Vectors

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Reporter Vectors Ready to Use in Your Signaling Pathway Without Cloning

  • Offer a number of response elements that are involved in nuclear response, kinase and other signaling pathways
  • Each vector included an optimized firefly luciferase gene that codes a reporter with a one-hour half-life
  • Hygromycin resistance selectable marker means choosing transient transfection or generating a stable cell line

Choose a response element

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Additional Signaling Pathway Analysis Response Element Firefly Luciferase Vectors
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$ 450.00
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Creating a cell line with an indicator of a functional signaling pathway is useful for deciphering the components in a signaling pathway. These tools are made by inserting multiple repeats of a response element upstream of a minimal promoter (minP). We have designed vectors that report the activity of a variety of pathways using the optimized luc2 firefly luciferase gene in the pGL4 backbone. The luc2 gene is engineered to remove most cryptic transcription factor binding sites and improve mammalian expression through codon optimization. The luc2P and Rapid Response™ genes are luc2 genes appended with degradation sequences to influence the cellular half-life of firefly luciferase. Proteins encoded by the Rapid Response™ genes respond more rapidly to stimuli but at the expense of signal intensity. The luc2P (1-hour half-life) gene responds more rapidly than luc2 (3-hour half-life) with moderate signal intensity.

These response element vectors also have a hygromycin resistance selectable marker for use either in transient transfection experiments or for selection of a stable cell line. Some of these response element vectors also are available stable cell lines (GloResponse™ Cell Lines) to speed your research. For investigating signaling pathways, we have vectors available that respond to the MAPK/JNK pathway with the pGL4.44[luc2P/AP1 RE/Hygro] Vector, Wnt pathway with the pGL4.49[luc2P/TCF-LEF RE/Hygro] Vector, cAMP/PKA pathway with the pGL4.29[luc2P/CRE/Hygro] Vector, Calcium/Calcineurin pathway with the pGL4.30[luc2P/NFAT-RE/Hygro] Vector, NF-κB pathway with the pGL4.32[luc2P/NF-κB-RE/Hygro] Vector, MAP/ERK pathway with the pGL4.33[luc2P/SRE/Hygro] Vector and RhoA pathway with the pGL4.34[luc2P/SRF-RE/Hygro] Vector.

Investigate additional signaling pathways with vectors for stress pathways and cytokine pathways.

Applications

  • Transcription regulation.
  • Virus-cell interactions.
  • Compound screening.
  • Post-translational modifications.
  • GPCR signaling.
  • Cell signaling.

Sequence Information

pGL4.29[luc2P/CRE/Hygro] Vector Accession Number DQ904461 and vector sequence text file.

pGL4.30[luc2P/NFAT-RE/Hygro] Vector Accession Number DQ904462 and vector sequence text file.

pGL4.32[luc2P/NF-κB-RE/Hygro] Vector Accession Number EU581860 and vector sequence text file.

pGL4.33[luc2P/SRE/Hygro] Vector Accession Number FJ773212 and vector sequence text file.

pGL4.34[luc2P/SRF-RE/Hygro] Vector Accession Number FJ773213 and vector sequence text file.

pGL4.44[luc2P/AP1 RE/Hygro] Vector Accession Number JQ858516 and vector sequence text file.

pGL4.49[luc2P/TCF-LEF RE/Hygro] Vector Accession Number JX099537 and vector sequence text file.

Activator/Pathway Transcription Factor Binding Site Vector Name Example Data
cAMP/PKA
CREB
Cyclic AMP Response Element
pGL4.29[luc2P/CRE/Hygro]
6045MC_PanelA_450px
High levels of reporter induction for cAMP Response Element containing vector in transient transfections. pGL4.29 Vector was transiently transfected into HEK293 cells. After 24 hours, reporter gene expression was induced by adding 100uM forskolin for 5 hours. Noninduced controls were treated with an equivalent amount of DMSO. Luciferase activity was measured using the Bright-Glo™ Luciferase Assay System (Cat.# E2610). Results will vary depending on the cell line and the transfection protocol used.
NF-κB
NF-κB
Nuclear Factor κB Response Element
pGL4.32[luc2P/NF-κB-RE/Hygro]
6045MC_PanelC_450px
High levels of reporter induction for Nuclear Factor κB (NF- κB) Response Element containing vector in transient transfections. pGL4.32 Vector was transiently transfected into HEK293 cells. After 24 hours, reporter gene expression was induced by 20ng/ml of TNFα for 5 hours. Noninduced controls were treated with an equivalent amount of standard media. Luciferase activity was measured using the ONE-Glo™ Luciferase Assay System (Cat.# E6110). Results will vary depending on the cell line and the transfection protocol used.
Calcium/Calcineurin
NFAT
Nuclear Factor of Activated T-Cells (NFAT) Response Element
pGL4.30[luc2P/NFAT-RE/Hygro]
6045MC_PanelB_450px

High levels of reporter induction for Nuclear Factor of Activated T-cells (NFAT) Response Element containing vector in transient transfections. pGL4.30 Vector was transiently transfected into HEK293 cells. After 24 hours, reporter gene expression was induced by adding 1μM ionomycin plus 10ng/ml PMA for 17 hours. Noninduced controls were treated with an equivalent amount of DMSO. Luciferase activity was measured using the Bright-Glo™ Luciferase Assay System (Cat.# E2610). Results will vary depending on the cell line and the transfection protocol used.

MAP/ERK
Elk-1/SRF
Serum Response Element
pGL4.33[luc2P/SRE/Hygro]
6045MC_PanelD_450px
High levels of reporter induction for Serum Response Element (SRE) containing vector in transient transfections. pGL4.33 Vector was transiently transfected into HEK293 cells. Four hours after transfection, cells were changed into serum-starved media. After 24 hours following transfection, reporter gene expression was induced 20% FBS plus 10ng/ml PMA for 6 hours. Noninduced controls were treated with an equivalent amount of standard media. Luciferase activity was measured using the ONE-Glo™ Luciferase Assay System (Cat.# E6110). Results will vary depending on the cell line and the transfection protocol used.
RhoA
SRF
Serum Response Factor Response Element
pGL4.34[luc2P/SRF-RE/Hygro]
6045MC_PanelE_450px
High levels of reporter induction for Serum Response Factor Response Element (SRF-RE) containing vector in transient transfections. pGL4.34 Vector was transiently transfected into HEK293 cells. Four hours after transfection, cells were changed into serum-starved media. After 24 hours following transfection, reporter gene expression was induced 20% FBS for 6 hours. Noninduced controls were treated with an equivalent amount of standard media. Luciferase activity was measured using the ONE-Glo™ Luciferase Assay System (Cat.# E6110). Results will vary depending on the cell line and the transfection protocol used.
MAPK/JNK
AP1
AP1 Response Element (AP1 RE)
pGL4.44 [luc2P/AP1 RE/Hygro]
10719MA_425px
Representative data for pGL4.44[luc2P/AP1 RE/Hygro] Vector in HEK293 cells upon stimulation with PMA. HEK293 cells were transiently transfected with pGL4.44[luc2P/AP1 RE/Hygro] and pGL4.75[hRluc/CMV] Vectors and assayed in a 96-well format as indicated in the protocol after six hours stimulation with PMA. Firefly luciferase luminescence normalized to the Renilla luciferase control is shown, with error bars indicating the S.E.M. for five replicates. Luminescence was detected after addition of Dual-Glo® reagents, using a GloMax® 96 instrument with a 0.5 second integration time.
Wnt
TCF-LEF
TCF-LEF Response Element (TCF-LEF RE)
pGL4.49[luc2P/TCF-LEF RE/Hygro]
10812MA_465px
Representative data for pGL4.49[luc2P/TCF-LEF RE/Hygro] Vector in HEK293 cells upon stimulation with mWnt3a or LiCl. HEK293 cells were transiently transfected with pGL4.49[luc2P/TCF-LEF RE/Hygro] and assayed in 96-well format after 8 hours stimulation with mWnt3a or LiCl as indicated in the protocol. Firefly luciferase luminescence normalized to untreated cells is shown, with error bars indicating S.E.M. for three replicates. Luminescence was detected after addition of ONE-Glo™ Reagent, using a GloMax® 96 instrument with a 0.5 second integration time.
5257ML2

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Item Part # Presentación Concentración
pGL4.44[luc2P/AP1 RE/Hygro] Vector E411A 1 × 20μg 1μg/μl

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Condiciones de Almacenaje

BB

Patentes y Exclusiones

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If the researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund.
Researchers may use this product for research use only, no commercial use is allowed. "Commercial use" means any and all uses of this product and derivatives by a party for money or other consideration and may include but is not limited to use in: (1) product manufacture; and (2) to provide a service, information or data; and/or resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene except that researchers may: (1) create fused gene sequences provided that the coding sequence of the resulting luciferase gene has no more than four deoxynucleotides missing at the affected terminus compared to the intact luciferase gene sequence, and (2) insert and remove nucleic acid sequences in splicing research predicated on the inactivation or reconstitution of the luminescence of the encoded luciferase. No other use or transfer of this product or derivatives is authorized without the prior express written consent of Promega. In addition, researchers must either: (1) use luminescent assay reagents purchased from Promega for all determinations of luminescence activity of this product and its derivatives; or (2) contact Promega to obtain a license for use of the product and its derivatives. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA. This label license relates to Promega patents and/or patent applications on improvements to the luciferase gene.

U.S. Pat. No. 8,008,006.

U.S. Pat. No. 7,728,118.

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pGL4.49[luc2P/TCF-LEF RE/Hygro] Vector E461A 1 × 20μg 1μg/μl

Certificado de Análisis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Condiciones de Almacenaje

BB

Patentes y Exclusiones

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If the researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund.
Researchers may use this product for research use only, no commercial use is allowed. "Commercial use" means any and all uses of this product and derivatives by a party for money or other consideration and may include but is not limited to use in: (1) product manufacture; and (2) to provide a service, information or data; and/or resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene except that researchers may: (1) create fused gene sequences provided that the coding sequence of the resulting luciferase gene has no more than four deoxynucleotides missing at the affected terminus compared to the intact luciferase gene sequence, and (2) insert and remove nucleic acid sequences in splicing research predicated on the inactivation or reconstitution of the luminescence of the encoded luciferase. No other use or transfer of this product or derivatives is authorized without the prior express written consent of Promega. In addition, researchers must either: (1) use luminescent assay reagents purchased from Promega for all determinations of luminescence activity of this product and its derivatives; or (2) contact Promega to obtain a license for use of the product and its derivatives. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA. This label license relates to Promega patents and/or patent applications on improvements to the luciferase gene.

U.S. Pat. No. 8,008,006.

U.S. Pat. No. 7,728,118.

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pGL4.29[luc2P/CRE/Hygro] Vector

E847A 1 × 20μg

Certificado de Análisis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Condiciones de Almacenaje

BB

Patentes y Exclusiones

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If the researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund.
Researchers may use this product for research use only, no commercial use is allowed. "Commercial use" means any and all uses of this product and derivatives by a party for money or other consideration and may include but is not limited to use in: (1) product manufacture; and (2) to provide a service, information or data; and/or resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene except that researchers may: (1) create fused gene sequences provided that the coding sequence of the resulting luciferase gene has no more than four deoxynucleotides missing at the affected terminus compared to the intact luciferase gene sequence, and (2) insert and remove nucleic acid sequences in splicing research predicated on the inactivation or reconstitution of the luminescence of the encoded luciferase. No other use or transfer of this product or derivatives is authorized without the prior express written consent of Promega. In addition, researchers must either: (1) use luminescent assay reagents purchased from Promega for all determinations of luminescence activity of this product and its derivatives; or (2) contact Promega to obtain a license for use of the product and its derivatives. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA. This label license relates to Promega patents and/or patent applications on improvements to the luciferase gene.

Patent Pending.

U.S. Pat. No. 8,008,006.

U.S. Pat. No. 7,728,118.

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pGL4.30[luc2P/NFAT-RE/Hygro] Vector

E848A 1 × 20μg

Certificado de Análisis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Condiciones de Almacenaje

BB

Patentes y Exclusiones

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If the researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund.
Researchers may use this product for research use only, no commercial use is allowed. "Commercial use" means any and all uses of this product and derivatives by a party for money or other consideration and may include but is not limited to use in: (1) product manufacture; and (2) to provide a service, information or data; and/or resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene except that researchers may: (1) create fused gene sequences provided that the coding sequence of the resulting luciferase gene has no more than four deoxynucleotides missing at the affected terminus compared to the intact luciferase gene sequence, and (2) insert and remove nucleic acid sequences in splicing research predicated on the inactivation or reconstitution of the luminescence of the encoded luciferase. No other use or transfer of this product or derivatives is authorized without the prior express written consent of Promega. In addition, researchers must either: (1) use luminescent assay reagents purchased from Promega for all determinations of luminescence activity of this product and its derivatives; or (2) contact Promega to obtain a license for use of the product and its derivatives. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA. This label license relates to Promega patents and/or patent applications on improvements to the luciferase gene.

Patent Pending.

U.S. Pat. No. 8,008,006.

U.S. Pat. No. 7,728,118.

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You are viewing: E8491 Cambiar Configuración

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pGL4.32[luc2P/NF-κB-RE/Hygro] Vector

E849A 1 × 20μg

Certificado de Análisis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Condiciones de Almacenaje

BB

Patentes y Exclusiones

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If the researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund.
Researchers may use this product for research use only, no commercial use is allowed. "Commercial use" means any and all uses of this product and derivatives by a party for money or other consideration and may include but is not limited to use in: (1) product manufacture; and (2) to provide a service, information or data; and/or resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene except that researchers may: (1) create fused gene sequences provided that the coding sequence of the resulting luciferase gene has no more than four deoxynucleotides missing at the affected terminus compared to the intact luciferase gene sequence, and (2) insert and remove nucleic acid sequences in splicing research predicated on the inactivation or reconstitution of the luminescence of the encoded luciferase. No other use or transfer of this product or derivatives is authorized without the prior express written consent of Promega. In addition, researchers must either: (1) use luminescent assay reagents purchased from Promega for all determinations of luminescence activity of this product and its derivatives; or (2) contact Promega to obtain a license for use of the product and its derivatives. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA. This label license relates to Promega patents and/or patent applications on improvements to the luciferase gene.

U.S. Pat. No. 8,008,006.

U.S. Pat. No. 7,728,118.

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You are viewing: E1340 Cambiar Configuración

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pGL4.33[luc2P/SRE/Hygro] Vector

E134A 1 × 20μg

Certificado de Análisis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Condiciones de Almacenaje

BB

Patentes y Exclusiones

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If the researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund.
Researchers may use this product for research use only, no commercial use is allowed. "Commercial use" means any and all uses of this product and derivatives by a party for money or other consideration and may include but is not limited to use in: (1) product manufacture; and (2) to provide a service, information or data; and/or resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene except that researchers may: (1) create fused gene sequences provided that the coding sequence of the resulting luciferase gene has no more than four deoxynucleotides missing at the affected terminus compared to the intact luciferase gene sequence, and (2) insert and remove nucleic acid sequences in splicing research predicated on the inactivation or reconstitution of the luminescence of the encoded luciferase. No other use or transfer of this product or derivatives is authorized without the prior express written consent of Promega. In addition, researchers must either: (1) use luminescent assay reagents purchased from Promega for all determinations of luminescence activity of this product and its derivatives; or (2) contact Promega to obtain a license for use of the product and its derivatives. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA. This label license relates to Promega patents and/or patent applications on improvements to the luciferase gene.

U.S. Pat. No. 8,008,006.

U.S. Pat. No. 7,728,118.

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You are viewing: E1350 Cambiar Configuración

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Item Part # Presentación

pGL4.34[luc2P/SRF-RE/Hygro] Vector

E135A 1 × 20μg

Certificado de Análisis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Condiciones de Almacenaje

BB

Patentes y Exclusiones

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If the researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund.
Researchers may use this product for research use only, no commercial use is allowed. "Commercial use" means any and all uses of this product and derivatives by a party for money or other consideration and may include but is not limited to use in: (1) product manufacture; and (2) to provide a service, information or data; and/or resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene except that researchers may: (1) create fused gene sequences provided that the coding sequence of the resulting luciferase gene has no more than four deoxynucleotides missing at the affected terminus compared to the intact luciferase gene sequence, and (2) insert and remove nucleic acid sequences in splicing research predicated on the inactivation or reconstitution of the luminescence of the encoded luciferase. No other use or transfer of this product or derivatives is authorized without the prior express written consent of Promega. In addition, researchers must either: (1) use luminescent assay reagents purchased from Promega for all determinations of luminescence activity of this product and its derivatives; or (2) contact Promega to obtain a license for use of the product and its derivatives. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA. This label license relates to Promega patents and/or patent applications on improvements to the luciferase gene.

U.S. Pat. No. 8,008,006.

U.S. Pat. No. 7,728,118.

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