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Stress Signaling Pathway Analysis Response Element Firefly Luciferase Vectors

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Firefly Luciferase Reporter Vectors with Cellular Stress Response Elements

  • Offer a range of response elements that are involved in stress signalling pathways
  • Each vector included an optimized firefly luciferase gene that codes a reporter with a one-hour half-life
  • Hygromycin resistance selectable marker means choosing transient transfection or generating a stable cell line

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Stress Signaling Pathway Analysis Response Element Firefly Luciferase Vectors
ARE/20µg
$ 450.00
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Creating a cell line with an indicator of a functional signaling pathway is useful for deciphering the components in a signaling pathway. These tools are made by insertion of multiple repeats of a response element upstream of a minimal promoter (minP). Promega has designed vectors that report the activity of a variety of pathways using the optimized luc2 firefly luciferase gene in the pGL4 backbone. These vectors also have a hygromycin resistance selectable marker, allowing use either in transient transfection experiments or for selection of a stable cell line. In addition, some of these predesigned response element vectors also are available stable cell lines (GloResponse™ Cell Lines) to speed your research.

There are more vectors for measuring cytokine signaling pathways and additional signaling pathways.

Applications

  • Cell transcription.
  • Virus-cell interactions.
  • Compound screening.
  • Post-translational modifications.
  • GPCR signaling.
  • Cell signaling.

About the pGL4 Reporter Vectors

The pGL4 Vectors offer increased reporter gene expression with codon optimization of synthetic genes for mammalian expression and reduced background and risk of expression artifacts with removal of cryptic DNA regulatory elements and transcription factor binding sites. Firefly luciferase has options that offer improved temporal response with the destabilized Rapid Response™ luciferase genes.

Sequence Information

pGL4.37[luc2P/ARE/Hygro] Vector GenBank® Accession Number JQ858521 and vector sequence text file.

pGL4.38[luc2P/p53 RE/Hygro] Vector GenBank® Accession Number JQ858522 and vector sequence text file.

pGL4.39[luc2P/ATF6 RE/Hygro] Vector GenBank® Accession Number JQ858519 and vector sequence text file.

pGL4.40[luc2P/MRE/Hygro] Vector GenBank® Accession Number JQ858515 and vector sequence text file.

pGL4.41[luc2P/HSE/Hygro] Vector GenBank® Accession Number JQ858520 and vector sequence text file.

pGL4.42[luc2P/HRE/Hygro] Vector GenBank® Accession Number JQ858518  and vector sequence text file.

pGL4.43[luc2P/XRE/Hygro] Vector GenBank® Accession Number JQ858513 and vector sequence text file.

Activator/Pathway Transcription Factor Binding Site Vector Name Example Data
Oxidative stress

Nrf2

Antioxidant Response Element (ARE)
pGL4.37[luc2P/ARE/Hygro]
10720MA_435px

Representative data for pGL4.37[luc2P/ARE/Hygro] Vector in HEK293 cells upon stimulation with tBHQ or D,L-Sulforaphane. HEK293 cells were transiently transfected with pGL4.37[luc2P/ARE/Hygro] and pGL4.75[hRluc>/CMV] Vectors and assayed in 96-well format after 18 hours stimulation with tBHQ or D,L-Sulforaphane as described. Firefly luciferase luminescence normalized to the Renilla> luciferase control is shown, with error bars indicating the S.E.M. for six replicates. Luminescence was detected after addition of Dual-Glo® reagents, using a GloMax® 96 instrument with a 0.5 second integration time.

DNA damage

p53

p53 response element (p53 RE)
pGL4.38[luc2P/p53 RE/Hygro]
10696MA_440px
Representative data for pGL4.38[luc2P/p53 RE/Hygro] Vector in U2OS cells upon stimulation with doxorubicin, etoposide, nutlin-3 and mitomycin c. U2OS cells were transiently transfected with the pGL4.38[luc2P/p53 RE/Hygro] and pGL4.75[hRluc/CMV] Vectors, and assayed in 96-well format after 18 hours stimulation with doxorubicin, nutlin-3, and etoposide, or after 40 hours with mitomycin c as indicated in the protocol. Firefly luciferase luminescence normalized to the Renilla luciferase control is shown, with error bars indicating the S.E.M. for six replicates. Luminescence was detected after addition of Dual-Glo® reagents, using a GloMax® 96 instrument with a 0.5 second integration time.
Endoplasmic reticulum stress
ATF6
Activating Transcription Factor 6 response element (ATF6 ERSE)
pGL4.39[luc2P/ATF6 RE/Hygro]
10811MA_435px
Representative data for pGL4.39[luc2P/ATF6 RE/Hygro] Vector in HeLa cells upon stimulation with tunicamycin. HeLa cells were transiently transfected with pGL4.39[luc2P/ATF6 RE/Hygro] and pGL4.75[hRluc/CMV] Vectors and assayed in 96-well format after 18 hours stimulation with tunicamycin as indicated in the protocol. Firefly luciferase luminescence normalized to the Renilla luciferase control is shown, with error bars indicating the S.E.M. for six replicates. Luminescence was detected after addition of Dual-Glo® reagents, using a GloMax® 96 instrument with a 0.5 second integration time.
Heavy metal stress
MTF1
Metal Regulatory Element (MRE)
pGL4.40[luc2P/MRE/Hygro]
10695MA_440px
Representative data for pGL4.40[luc2P/MRE/Hygro] Vector in HepG2 cells upon stimulation with ZnSO4. HepG2 cells were transiently transfected with pGL4.40[luc2P/MRE/Hygro] and pGL4.75[hRluc/CMV] Vectors and assayed in 96-well format after six hours stimulation with ZnSO4 as indicated in the protocol. Firefly luciferase luminescence normalized to the Renilla luciferase control is shown. Error bars indicate the S.E.M. for six replicates. Luminescence was detected after addition of Dual-Glo® reagents, using a GloMax® 96 instrument with a 0.5 second integration time.
Heat shock
HSF1
Heat Shock Element (HSE)
pGL4.41[luc2P/HSE/Hygro]
10724MA_500px
Representative data for pGL4.41[luc2P/HSE/Hygro] Vector in HepG2 cells upon stimulation with 17-AAG or CdCl2. HepG2 cells were transiently transfected with pGL4.41[luc2P/HSE/Hygro] and pGL4.75[hRluc/CMV] Vectors and assayed in 96-well format after six hours stimulation with 17-AAG or CdCl2 as indicated. Firefly luciferase luminescence normalized to the Renilla luciferase control is shown. Error bars indicate the S.E.M. for six replicates. Luminescence was detected after addition of Dual-Glo® reagents, using a GloMax® 96 instrument with a 0.5 second integration time.
Hypoxia
Hif1α
Hypoxia Response Element (HRE)
pGL4.42[luc2P/HRE/Hygro]
10723MA_420px
Representative data for pGL4.42[luc2P/HRE/Hygro] Vector in HEK293 cells upon stimulation with 1,10-phenanthroline. HEK293 cells were transiently transfected with pGL4.42[luc2P/HRE/Hygro] and pGL4.75[hRluc/CMV] Vectors, and assayed in 96-well format after five hours stimulation with 1,10-phenanthroline as indicated in the protocol. Firefly luciferase luminescence is shown, normalized to untreated cells, with error bars indicating the S.E.M. for six replicates. Luminescence was detected after addition of Dual-Glo® reagent, using a GloMax® 96 instrument with a 0.5 second integration time.
Xenobiotic stress

AhR

Xenobiotic Responsive Element (XRE)
pGL4.43[luc2P/XRE/Hygro]
10730MA_445px
Representative data for pGL4.43[luc2P/XRE/Hygro] Vector in HepG2 cells upon stimulation with TCDD. HepG2 cells were transiently transfected with pGL4.43[luc2P/XRE/Hygro] and pGL4.75[hRluc/CMV] Vectors and assayed in 96-well format after 24 hours stimulation with TCDD as indicated in the protocol. Firefly luciferase luminescence normalized to the Renilla luciferase control is shown, with error bars indicating the S.E.M. for six replicates. Luminescence was detected after addition of Dual-Glo® reagents, using a GloMax® 96 instrument with a 0.5 second integration time.

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pGL4.37[luc2P/ARE/Hygro] Vector E364A 1 × 20μg 1μg/μl

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Condiciones de Almacenaje

BB

Patentes y Exclusiones

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If the researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund.
Researchers may use this product for research use only, no commercial use is allowed. "Commercial use" means any and all uses of this product and derivatives by a party for money or other consideration and may include but is not limited to use in: (1) product manufacture; and (2) to provide a service, information or data; and/or resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene except that researchers may: (1) create fused gene sequences provided that the coding sequence of the resulting luciferase gene has no more than four deoxynucleotides missing at the affected terminus compared to the intact luciferase gene sequence, and (2) insert and remove nucleic acid sequences in splicing research predicated on the inactivation or reconstitution of the luminescence of the encoded luciferase. No other use or transfer of this product or derivatives is authorized without the prior express written consent of Promega. In addition, researchers must either: (1) use luminescent assay reagents purchased from Promega for all determinations of luminescence activity of this product and its derivatives; or (2) contact Promega to obtain a license for use of the product and its derivatives. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA. This label license relates to Promega patents and/or patent applications on improvements to the luciferase gene.

U.S. Pat. No. 8,008,006.

U.S. Pat. No. 7,728,118.

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Item Part # Presentación Concentración
pGL4.38[luc2P/p53 RE/Hygro] Vector E365A 1 × 20μg 1μg/μl

Certificado de Análisis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Condiciones de Almacenaje

BB

Patentes y Exclusiones

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If the researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund.
Researchers may use this product for research use only, no commercial use is allowed. "Commercial use" means any and all uses of this product and derivatives by a party for money or other consideration and may include but is not limited to use in: (1) product manufacture; and (2) to provide a service, information or data; and/or resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene except that researchers may: (1) create fused gene sequences provided that the coding sequence of the resulting luciferase gene has no more than four deoxynucleotides missing at the affected terminus compared to the intact luciferase gene sequence, and (2) insert and remove nucleic acid sequences in splicing research predicated on the inactivation or reconstitution of the luminescence of the encoded luciferase. No other use or transfer of this product or derivatives is authorized without the prior express written consent of Promega. In addition, researchers must either: (1) use luminescent assay reagents purchased from Promega for all determinations of luminescence activity of this product and its derivatives; or (2) contact Promega to obtain a license for use of the product and its derivatives. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA. This label license relates to Promega patents and/or patent applications on improvements to the luciferase gene.

U.S. Pat. No. 8,008,006.

U.S. Pat. No. 7,728,118.

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Item Part # Presentación Concentración
pGL4.39[luc2P/ATF6 RE/Hygro] Vector E366A 1 × 20μg 1μg/μl

Certificado de Análisis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Condiciones de Almacenaje

BB

Patentes y Exclusiones

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If the researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund.
Researchers may use this product for research use only, no commercial use is allowed. "Commercial use" means any and all uses of this product and derivatives by a party for money or other consideration and may include but is not limited to use in: (1) product manufacture; and (2) to provide a service, information or data; and/or resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene except that researchers may: (1) create fused gene sequences provided that the coding sequence of the resulting luciferase gene has no more than four deoxynucleotides missing at the affected terminus compared to the intact luciferase gene sequence, and (2) insert and remove nucleic acid sequences in splicing research predicated on the inactivation or reconstitution of the luminescence of the encoded luciferase. No other use or transfer of this product or derivatives is authorized without the prior express written consent of Promega. In addition, researchers must either: (1) use luminescent assay reagents purchased from Promega for all determinations of luminescence activity of this product and its derivatives; or (2) contact Promega to obtain a license for use of the product and its derivatives. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA. This label license relates to Promega patents and/or patent applications on improvements to the luciferase gene.

U.S. Pat. No. 8,008,006.

U.S. Pat. No. 7,728,118.

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pGL4.40[luc2P/MRE/Hygro] Vector E413A 1 × 20μg 1μg/μl

Certificado de Análisis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Condiciones de Almacenaje

BB

Patentes y Exclusiones

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If the researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund.
Researchers may use this product for research use only, no commercial use is allowed. "Commercial use" means any and all uses of this product and derivatives by a party for money or other consideration and may include but is not limited to use in: (1) product manufacture; and (2) to provide a service, information or data; and/or resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene except that researchers may: (1) create fused gene sequences provided that the coding sequence of the resulting luciferase gene has no more than four deoxynucleotides missing at the affected terminus compared to the intact luciferase gene sequence, and (2) insert and remove nucleic acid sequences in splicing research predicated on the inactivation or reconstitution of the luminescence of the encoded luciferase. No other use or transfer of this product or derivatives is authorized without the prior express written consent of Promega. In addition, researchers must either: (1) use luminescent assay reagents purchased from Promega for all determinations of luminescence activity of this product and its derivatives; or (2) contact Promega to obtain a license for use of the product and its derivatives. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA. This label license relates to Promega patents and/or patent applications on improvements to the luciferase gene.

U.S. Pat. No. 8,008,006.

U.S. Pat. No. 7,728,118.

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pGL4.41[luc2P/HSE/Hygro] Vector E375A 1 × 20μg 1μg/μl

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Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Condiciones de Almacenaje

BB

Patentes y Exclusiones

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If the researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund.
Researchers may use this product for research use only, no commercial use is allowed. "Commercial use" means any and all uses of this product and derivatives by a party for money or other consideration and may include but is not limited to use in: (1) product manufacture; and (2) to provide a service, information or data; and/or resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene except that researchers may: (1) create fused gene sequences provided that the coding sequence of the resulting luciferase gene has no more than four deoxynucleotides missing at the affected terminus compared to the intact luciferase gene sequence, and (2) insert and remove nucleic acid sequences in splicing research predicated on the inactivation or reconstitution of the luminescence of the encoded luciferase. No other use or transfer of this product or derivatives is authorized without the prior express written consent of Promega. In addition, researchers must either: (1) use luminescent assay reagents purchased from Promega for all determinations of luminescence activity of this product and its derivatives; or (2) contact Promega to obtain a license for use of the product and its derivatives. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA. This label license relates to Promega patents and/or patent applications on improvements to the luciferase gene.

U.S. Pat. No. 8,008,006.

U.S. Pat. No. 7,728,118.

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You are viewing: E4001 Cambiar Configuración

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pGL4.42[luc2P/HRE/Hygro] Vector E400A 1 × 20μg 1μg/μl

Certificado de Análisis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Condiciones de Almacenaje

BB

Patentes y Exclusiones

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If the researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund.
Researchers may use this product for research use only, no commercial use is allowed. "Commercial use" means any and all uses of this product and derivatives by a party for money or other consideration and may include but is not limited to use in: (1) product manufacture; and (2) to provide a service, information or data; and/or resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene except that researchers may: (1) create fused gene sequences provided that the coding sequence of the resulting luciferase gene has no more than four deoxynucleotides missing at the affected terminus compared to the intact luciferase gene sequence, and (2) insert and remove nucleic acid sequences in splicing research predicated on the inactivation or reconstitution of the luminescence of the encoded luciferase. No other use or transfer of this product or derivatives is authorized without the prior express written consent of Promega. In addition, researchers must either: (1) use luminescent assay reagents purchased from Promega for all determinations of luminescence activity of this product and its derivatives; or (2) contact Promega to obtain a license for use of the product and its derivatives. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA. This label license relates to Promega patents and/or patent applications on improvements to the luciferase gene.

U.S. Pat. No. 8,008,006.

U.S. Pat. No. 7,728,118.

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You are viewing: E4121 Cambiar Configuración

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pGL4.43[luc2P/XRE/Hygro] Vector E412A 1 × 20μg 1μg/μl

Certificado de Análisis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Condiciones de Almacenaje

BB

Patentes y Exclusiones

BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If the researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund.
Researchers may use this product for research use only, no commercial use is allowed. "Commercial use" means any and all uses of this product and derivatives by a party for money or other consideration and may include but is not limited to use in: (1) product manufacture; and (2) to provide a service, information or data; and/or resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene except that researchers may: (1) create fused gene sequences provided that the coding sequence of the resulting luciferase gene has no more than four deoxynucleotides missing at the affected terminus compared to the intact luciferase gene sequence, and (2) insert and remove nucleic acid sequences in splicing research predicated on the inactivation or reconstitution of the luminescence of the encoded luciferase. No other use or transfer of this product or derivatives is authorized without the prior express written consent of Promega. In addition, researchers must either: (1) use luminescent assay reagents purchased from Promega for all determinations of luminescence activity of this product and its derivatives; or (2) contact Promega to obtain a license for use of the product and its derivatives. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA. This label license relates to Promega patents and/or patent applications on improvements to the luciferase gene.

U.S. Pat. No. 8,008,006.

U.S. Pat. No. 7,728,118.

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