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Dual-Luciferase® Reporter Assay System

E1910_Dual-Luciferase--Reporter-Assay-System_3
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Detect firefly and Renilla Luciferase Activities in a Single Sample

  • Renilla luciferase internal control provides more accurate results
  • Range extends over 7 logs; very active samples typically do not need dilution
  • Requires luminometer with 2 injectors

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Catalog number selected: E1910

$ 250.00
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Dual-Luciferase® Reporter Assay System
100 assays
$ 250.00
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Sensitive, Rapid and Convenient Dual-Reporter Assay

The Dual-Luciferase® Reporter (DLR™) Assay System provides an efficient means of performing two reporter assays. In the DLR™ Assay, the activities of firefly (Photinus pyralis) and Renilla (Renilla reniformis or sea pansy) luciferases are measured sequentially from a single sample.

The firefly luciferase reporter is measured first by adding Luciferase Assay Reagent II (LAR II) to generate a luminescent signal lasting at least one minute. After quantifying the firefly luminescence, this reaction is quenched, and the Renilla luciferase reaction is initiated simultaneously by adding Stop & Glo® Reagent to the same sample. Both assays can be completed in about 4 seconds using a luminometer with reagent auto-injectors. In the DLR™ Assay System, both reporters yield linear assays with attomole (<10–18) sensitivities and no endogenous activity in the experimental host cells. Furthermore, the integrated format of the DLR™ Assay provides rapid quantitation of both reporters either in transfected cells or in cell-free transcription/translation reactions.

For best results with the Dual-Luciferase® Assay, we recommend using a luminometer that has been validated for use with the assay. These luminometers are qualified as DLReady™. For a listing of qualified instruments, please visit the DLReady™ Validated Luminometers page.

The pGL4 Luciferase Reporter Vectors are designed for use with the DLR™ Assay Systems. A Renilla luciferase vector with constitutive expression may be used in combination with any experimental firefly luciferase vector to co-transfect mammalian cells.

Notice for Cat.# E1960 and E1980: Sufficient Passive Lysis Buffer is provided to perform 1,000 assays with cells grown in 96-well plates (typically 20μl of 1X PLB per well). For applications requiring more lysis reagent (e.g., >100μl/well), additional Passive Lysis Buffer may be purchased separately.

Assay Advantages

  • Normalization to Renilla luciferase internal control allows more accurate results.
  • Samples don't have to be split, saving plates and time.
  • Allows study of weak promoters, low-level expression/regulation and expression in cells that transfect poorly.
  • Linear over 7 logs; very active samples typically do not need dilution.

Especificaciones

You are viewing: E1910 Cambiar Configuración

Contenido

Item Part # Presentación Available Separately

Luciferase Assay Substrate

E151A 1 × 1 vial

Passive Lysis Buffer, 5X

E194A 1 × 30ml View Product

Luciferase Assay Buffer II

E195A 1 × 10ml

Stop & Glo® Substrate

E640A 1 × 200μl

Stop & Glo® Buffer

E641A 1 × 10ml

Certificado de Análisis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Condiciones de Almacenaje

BB

Patentes y Exclusiones

U.S. Pat. Nos. 7,078,181, 7,108,996, 7,118,878 and other patents.

Especificaciones

You are viewing: E1960 Cambiar Configuración

Contenido

Item Part # Presentación Available Separately

Luciferase Assay Substrate

E151A 10 × 1 vial

Passive Lysis Buffer, 5X

E194A 1 × 30ml View Product

Luciferase Assay Buffer II

E195A 10 × 10ml

Stop & Glo® Substrate

E640A 10 × 200μl

Stop & Glo® Buffer

E641A 10 × 10ml

Certificado de Análisis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Condiciones de Almacenaje

BB

Patentes y Exclusiones

U.S. Pat. Nos. 7,078,181, 7,108,996, 7,118,878 and other patents.

Especificaciones

You are viewing: E1980 Cambiar Configuración

Contenido

Item Part # Presentación Available Separately

Stop & Glo® Reagent Bottle

E118C 1 × 1 each

Luciferase Assay Substrate

E151C 1 × 1 vial

Passive Lysis Buffer, 5X

E194A 1 × 30ml View Product

Luciferase Assay Buffer II

E195C 1 × 105ml

Stop & Glo® Substrate

E640B 2 × 1.05ml

Stop & Glo® Buffer

E641B 1 × 105ml

Certificado de Análisis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Condiciones de Almacenaje

BB

Patentes y Exclusiones

U.S. Pat. Nos. 7,078,181, 7,108,996, 7,118,878 and other patents.

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