FuGENE® SI Transfection Reagent

High-Efficiency, Gentle Reagent for Small RNA Delivery

FuGENE® SI is an innovative, 100% synthetic, multicomponent transfection reagent designed for the delivery of siRNA, miRNA and other small RNA molecules. FuGENE® SI provides high-efficiency delivery of RNA into a wide range of eukaryotic cell lines while providing the gentle, low-toxicity transfection you expect from FuGENE® reagents. Obtain maximum knockdown efficiencies while minimizing impact on cell health, allowing you to get the most out of transfection-based experiments.

Animal-free reagents and strong performance make FuGENE® SI ideal for a variety of RNA transfection applications from functional genomics to siRNA library screens.

The quick and easy protocol allows you to obtain superior results with minimal optimization, freeing-up valuable lab time.

Gentle and Nontoxic siRNA Transfection

FuGENE® SI Transfection Reagent delivers gentle and nontoxic siRNA transfection. HEK293-GFP and NIH3T3-GFP cell lines (Cell Biolabs) were seeded in 96-well plates according to user guides and subsequently transfected with 0.2, 1.0 or 5pmol of GFP targeting siRNA or negative control (Dharmacon™, Horizon) along with 0.3µl of FuGENE® SI Transfection Reagent. Cells were then analyzed via flow cytometry 48 hours post-transfection to measure total cell viability. Graphs show the gentle and nontoxic transfection of siRNAs using FuGENE® SI.

Superior Knockdown Efficiency

Bar graph illustrating that FuGENE® SI outperforms Lipofectamine™ RNAiMAX in siRNA knockdown efficiency demonstrated using HEK293-GFP cell line.

Bar graph illustrating that FuGENE® SI outperforms Lipofectamine™ RNAiMAX in siRNA knockdown efficiency demonstrated using NIH3T3-GFP cell line.

FuGENE® SI outperforms Lipofectamine™ RNAiMAX in siRNA knockdown efficiency. HEK293-GFP and NIH3T3-GFP cell lines (Cell Biolabs) were seeded in 96-well plates according to user guides and subsequently transfected with 0.2, 1.0 or 5pmol of GFP targeting siRNA or negative control (Dharmacon™, Horizon) along with 0.3µl of FuGENE® SI Transfection Reagent or 0.3µl of Lipofectamine™ RNAiMAX (Life Technologies). Cells were then analyzed via flow cytometry 48 hours post-transfection to measure % knockdown of GFP and total cell viability. Graphs show the superior knockdown performance of FuGENE® SI versus Lipofectamine™ RNAiMAX in HEK293 and NIH3T3 cells.

FuGENE® SI outperforms Lipofectamine™ RNAiMAX in siRNA knockdown efficiency, as confirmed by Western blot. Human MDA-MB-468 cells were reverse-transfected in 6-well plates with 40pmol of the indicated targeting siRNAs or negative/positive control (Silencer® Select siRNA, Invitrogen) utilizing either 7.5µl of FuGENE® SI Transfection Reagent or 7.5µl of Lipofectamine™ RNAiMAX per well. The cells were collected 72 hours after transfection and analyzed via Western blot for expression of target genes STAT3 and CEBPB. The Western blot shown here demonstrates the robust, superior knockdown with FuGENE® SI as compared to the market leading competitor.

Efficient Transfection of Labeled Small RNAs

FuGENE® SI provides efficient transfection of labeled small RNAs. A549, COS-7, HEK293 and HeLa cells plated in 96-well microplates were transfected with 0.4µl of FuGENE® SI or a no-reagent negative control and various amounts of FITC-labeled negative control siRNA. Twenty-four hours after transfection, the cells were analyzed via flow cytometry for FITC-positive signal.

Protocolos

Complete Protocol

FuGENE® SI Transfection Reagent Technical ManualPDF (1585 KB) – English

Contenido

Item	Part #	Presentación
FuGENE® SI Transfection Reagent	E931A	1 × 1ml

SDS

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Certificado de Análisis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Condiciones de Almacenaje

Patentes y Exclusiones

FuGENE is a registered trademark of Fugent, L.L.C., USA.

FuGENE® SI is sold with a Limited Use Label License (LULL).

You shall use this Product in accordance with all applicable laws, rules and regulations. You may not attempt to reverse engineer this Product. If you are not willing to accept these conditions, the distributor from whom the Product was purchased will accept the return of the unopened Product and provide you with a full refund. However, in the event this Product is opened, you agree to be bound by the conditions of this limited use statement.

Contenido

Item	Part #	Presentación
FuGENE® SI Transfection Reagent	E931A	5 × 1ml

SDS

Search for SDS

Certificado de Análisis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Condiciones de Almacenaje

Patentes y Exclusiones

FuGENE is a registered trademark of Fugent, L.L.C., USA.

FuGENE® SI is sold with a Limited Use Label License (LULL).

You shall use this Product in accordance with all applicable laws, rules and regulations. You may not attempt to reverse engineer this Product. If you are not willing to accept these conditions, the distributor from whom the Product was purchased will accept the return of the unopened Product and provide you with a full refund. However, in the event this Product is opened, you agree to be bound by the conditions of this limited use statement.

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FuGENE® SI Transfection Reagent

Versatile Transfection Reagent for siRNA, miRNA and Other Small RNA Molecules

High-Efficiency, Gentle Reagent for Small RNA Delivery

Gentle and Nontoxic siRNA Transfection

Superior Knockdown Efficiency

Efficient Transfection of Labeled Small RNAs

Protocolos

Complete Protocol

Especificaciones

Contenido

SDS

Certificado de Análisis

Use Restrictions

Condiciones de Almacenaje

Patentes y Exclusiones

Especificaciones

Contenido

SDS

Certificado de Análisis

Use Restrictions

Condiciones de Almacenaje

Patentes y Exclusiones

FuGENE® 4K Transfection Reagent

FuGENE® HD Transfection Reagent

FuGENE® 6 Transfection Reagent

ViaFect™ Transfection Reagent

ProFection® Mammalian Transfection System

QuantiFluor® RNA System

RiboMAX™ Express RNAi System

psiCHECK™-2 Vector

pmirGLO Dual-Luciferase miRNA Target Expression Vector

GoTaq® qPCR y RT-qPCR Systems

GloMax® Discover System

GoTaq® Probe qPCR and RT-qPCR Systems

CellTiter-Glo® Luminescent Cell Viability Assay

Steady-Glo® Luciferase Assay System

FuGENE® SI Transfection Reagent

Versatile Transfection Reagent for siRNA, miRNA and Other Small RNA Molecules​

High-Efficiency, Gentle Reagent for Small RNA Delivery​

Gentle and Nontoxic siRNA Transfection

Superior Knockdown Efficiency

Efficient Transfection of Labeled Small RNAs

Protocolos

Complete Protocol

Especificaciones

Contenido

SDS

Certificado de Análisis

Use Restrictions

Condiciones de Almacenaje

Patentes y Exclusiones

Especificaciones

Contenido

SDS

Certificado de Análisis

Use Restrictions

Condiciones de Almacenaje

Patentes y Exclusiones

Productos Similares

FuGENE® 4K Transfection Reagent

FuGENE® HD Transfection Reagent

FuGENE® 6 Transfection Reagent

ViaFect™ Transfection Reagent

ProFection® Mammalian Transfection System

Usado con frecuencia junto con

QuantiFluor® RNA System

RiboMAX™ Express RNAi System

psiCHECK™-2 Vector

pmirGLO Dual-Luciferase miRNA Target Expression Vector

GoTaq® qPCR y RT-qPCR Systems

GloMax® Discover System

GoTaq® Probe qPCR and RT-qPCR Systems

CellTiter-Glo® Luminescent Cell Viability Assay

Steady-Glo® Luciferase Assay System

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Versatile Transfection Reagent for siRNA, miRNA and Other Small RNA Molecules

High-Efficiency, Gentle Reagent for Small RNA Delivery