Arg-C Ultra, Mass Spec Grade
Fast, Efficient and Ultra-Specific Cysteine Protease for Use Alone or With Other Proteases for Bottom-Up Proteomics
- Exclusively cleaves at the C-terminus of arginine residues including Arg-Pro sequence
- Negligible cleavage at lysine residues, unlike standard Arg-C enzymes such as clostripain
- Digests samples in as little as 30 minutes
- Exhibits excellent cleavage activity in up to 6M urea
- Robust digestion in a broad pH range from pH 5.0–9.0
- Excellent use in combination with Lys-C to produce “tryptic” peptides
Catalog Number:
Size
Arg-C Proteinase with Superior Cleavage Specificity and Efficiency for Mass Spec Analysis
The Arg-C Ultra protease is an endopeptidase that cleaves at the C-terminus of arginine residues, including the arginine amino acid next to the proline (Arg-Pro). This Mass Spec Grade enzyme can be used alone or in combination with other proteases for protein analysis by mass spectrometry and other applications. Arg-C Ultra has robust digestion activity with a pH range from pH 5.0–pH 9.0.
Applications
- Protein Identification (ID)
- Peptide mapping
- De novo peptide sequencing
- Characterization of post-translational modifications, particularly on lysines
- Characterization and QA/QC of biotherapeutics (e.g., IgGs)
Arg-C Ultra offers superior specificity and efficiency over standard Arg-C and Trypsin proteases. Human K562 extract was digested overnight at 37°C with the indicated proteases using a 1:100 enzyme-to-substrate ratio. Peptides were analyzed by LC-MS/MS on an Orbitrap Exploris™ 240 (Thermo Fisher Scientific). Data analyses were conducted using Byonic software (Protein Metrics) with no enzyme specified.
Effect of urea concentration on digestion efficiency of Arg-C Ultra, Mass Spec Grade, and Lys-C. Human K562 extract was digested with Arg-C Ultra, Mass Spec Grade, or native Lys-C at 1:100 for 2 hours at 37°C at a variety of urea concentrations. Peptides were analyzed by LC-MS/MS on an Orbitrap Exploris™ 240 (Thermo Fisher Scientific). Data analyses were conducted using Byonic software (Protein Metrics, Inc.) with no enzyme specified.
Effect of pH on digestion efficiency of Arg-C Ultra, Mass Spec Grade. Human K562 extract was digested with Arg-C Ultra, Mass Spec Grade, at 1:100 for 2 hours at 37°C at different pH concentrations. Peptides were analyzed by LC-MS/MS on an Orbitrap Exploris™ 240 (Thermo Fisher Scientific). Data analyses were conducted using Byonic software (Protein Metrics, Inc.) with no enzyme specified.
Protocols
Complete Protocol
Specifications
Catalog Number:
Contenido
| Item | Part # | Presentación | Concentración |
|---|---|---|---|
Arg-C Ultra, Mass Spec Grade |
VA183A | 1 × 5μg | 0.2μg/μl |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
Contenido
| Item | Part # | Presentación | Concentración |
|---|---|---|---|
Arg-C Ultra, Mass Spec Grade |
VA183B | 1 × 20μg | 0.2μg/μl |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
Resources
Featured Resource
Characterization of a new ultra-selective, highly active Arginine-C protease for MS-based proteomics
Arg-C Ultra is successful in various applications, including characterization of bulk proteomes, hard-to-digest samples like muscle tissue, and post-translational modifications, particularly at lysine residues.
Related Products
Productos Similares
Lys-C, Mass Spec Grade
Sitio de escisión: C-terminal de Lys. pH óptimo: 7-9.
VA1170
rAsp-N, Mass Spec Grade
Sitio de escisión: N-terminal de Asp. pH óptimo: 6-9.
VA1160
Glu-C, Sequencing Grade
Sitio de escisión: C-terminal de Glu. pH óptimo: 4-9.
V1651
PNGase F
Cataliza la escisión de oligosacáridos ligados a N entre los residuos más internos de GlcNAc y asparagina.
V4831
Usado con frecuencia junto con
Sequencing Grade Modified Trypsin
Tripsina de alta actividad y estabilidad fabricada para proporcionar la máxima especificidad.
V5111, V5117, V5113
Trypsin Gold, Mass Spectrometry Grade
Máxima especificidad de digestión con extrema resistencia a la digestión autolítica.
V5280
Trypsin/Lys-C Mix, Mass Spec Grade
Mayor recuperación de péptidos, mejor cuantificación de proteínas, mayor reproducibilidad.
V5071, V5072, V5073
rLys-C, Mass Spec Grade
Sitio de escisión: C-terminal de Lys. pH óptimo: 8-9.
V1671
Trypsin Platinum, Mass Spectrometry Grade
Máxima especificidad de digestión. Máxima resistencia a la autoproteólisis.
VA9000
Iodoacetamide
La yodoacetamida es un componente del AccuMAP Low pH Protein Digestion Kit.
VB1010
TCEP
El TCEP es un componente del AccuMAP Low pH Protein Digestion Kit.
VB1000
ProteaseMAX™ Surfactant, Trypsin Enhancer
Para mejorar la digestión en gel y la solubilización de proteínas.
V2071, V2072
