IdeS Protease and IdeZ Protease

V7511_IdeS-Protease--5000U_3
V8341_IdeZ-Protease--5000U_3
V7511_IdeS-Protease--5000U_3
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Antibody Fragmentation and Characterization, Simplified

  • Highly specific IgG cleavage
  • Rapid generation of highly pure fragments
  • Frozen IdeS Protease and Frozen IdeZ Protease are no longer available

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Catalog number selected: V7511

$ 1,064.00
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This product is available through the Promega Helix onsite stocking program. We offer numerous convenient solutions to meet your lab's needs.

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This product can be added to Helix

This product is available through the Promega Helix onsite stocking program. We offer numerous convenient solutions to meet your lab's needs.

Learn more about the Helix Collection »

This product can be added to Helix

This product is available through the Promega Helix onsite stocking program. We offer numerous convenient solutions to meet your lab's needs.

Learn more about the Helix Collection »

This product is available under our Early Access program - Learn More
This product is available under our Catalog (FT) program - Learn More
IdeS Protease and IdeZ Protease
IdeS Protease/5,000 units
$ 1,064.00
Your price: Acceder
Cambiar Configuración

IgG-Digesting Enzymes for Therapeutic Antibody Characterization

IdeS and IdeZ Proteases are immunoglobulin G (IgG)-degrading enzymes that are valuable tools for the characterization of therapeutic antibodies, Fc fusion proteins and antibody-drug conjugates.

IdeS and IdeZ Proteases are fast and easy-to-use; complete digestion can be achieved in 30 minutes without optimization. Cleavage is highly reproducible and specific at a single site below the hinge region.

Both IdeS and IdeZ Proteases effectively cleave human IgG1, IgG2, IgG3 and IgG4, monkey, sheep, rabbit, humanized and chimeric IgGs as well as Fc-fusion proteins. However, only IdeZ Protease cleaves mouse IgG2a and IgG3.

32609757-L1-IdeS-IdeZ-Foreground-2

Cleavage Specificity of IdeS and IdeZ Proteases

12335MB-W

IdeS and IdeZ Proteases are immunoglobulin G (IgG)-degrading proteases that cleave with high specificity.

IdeS Protease is derived from Streptococcus pyogenes. It is an engineered, recombinant protease overexpressed in E. coli. IdeS Protease cleaves IgG at a single site below the hinge region, yielding F(ab')2 and Fc fragments as shown here.

IdeZ Protease is derived from Streptococcus equi subspecies zooepidemicus. It is also an engineered, recombinant protease overexpressed in E. coli. Like IdeS, IdeZ Protease specifically cleaves IgG molecules below the hinge region, yielding F(ab')2 and Fc fragments. However, IdeZ Protease has significantly improved activity against mouse IgG2a and IgG3 subclasses compared to IdeS Protease.

Core and Lower Hinge Sequence of Human and Mouse IgG Subclasses

IdeS and IdeZ Protease activity against each subclass is indicated. Cut sites are indicated by bolded letters.
Subclass Hinge/CH2 Sequence IdeS Activity IdeZ Activity
Human
IgG1
CPPCPAPELLGGPSVF
+++
+++
IgG2
CPPCPAPP_VAGPSVF
+++
+++
IgG3
CPRCPAPELLGGPSVF
+++
+++
IgG4
AHHAQAPEFLGGPSVF
+++
+++
Subclass Hinge/CH2 Sequence IdeS Activity IdeZ Activity
Mouse
IgG1
PCICTVPEV_SSVF
–
–
IgG2a
CPPCAAPNLLGGPSVF
+
+++
IgG2b
CHKCPAPNLEGGPSVF
–
–
IgG3
GSSCPAGNILGGPSVF
+
+++

Preparation of F(ab′)2 Fragments using IdeS Protease and Magne™ Protein A Beads

12337MA-W-a-2

Panel A. Generation of F(ab′)2 fragments using IdeS Protease and Magne™ Protein A Beads.

12337MA-W-b-2

Panel B. Gels demonstrating separation of F(ab′)2 and Fc fragments from three antibodies. Fc fragments were captured using Magne™ Protein A beads.

Protocolos

Complete Protocol

Especificaciones

You are viewing: V7511 Cambiar Configuración

Contenido

Item Part # Presentación

IdeS Protease

 V751A 1 × 5,000 units

SDS

Search for SDS

Certificado de Análisis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Condiciones de Almacenaje

BB

Especificaciones

You are viewing: V7515 Cambiar Configuración

Contenido

Item Part # Presentación

IdeS Protease

 V751A 5 × 5,000 units

SDS

Search for SDS

Certificado de Análisis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Condiciones de Almacenaje

BB

Especificaciones

You are viewing: V8341 Cambiar Configuración

Contenido

Item Part # Presentación

IdeZ Protease

 V834A 1 × 5,000 units

SDS

Search for SDS

Certificado de Análisis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Condiciones de Almacenaje

GG

Especificaciones

You are viewing: V8345 Cambiar Configuración

Contenido

Item Part # Presentación

IdeZ Protease

 V834A 5 × 5,000 units

SDS

Search for SDS

Certificado de Análisis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Condiciones de Almacenaje

GG

Recursos

Menciones

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Encontremos el producto que se adapte a sus necesidades.

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