Protein Enrichment and Sample Cleanup
In bottom-up proteomics, effective protein enrichment and sample cleanup are essential for obtaining reliable and reproducible mass spectrometry (MS) results. These steps help remove unwanted contaminants such as salts, detergents and lipids while enriching target proteins or peptides, improving overall detection sensitivity. By employing techniques like single-pot, solid phase-enhanced sample preparation (SP3), protein aggregation capture (PAC), solid-phase extraction (SPE), ultrafiltration, immunoaffinity capture or co-immunoprecipitation, you can enhance digestion efficiency and reduce background noise. A well optimized sample preparation workflow ensures higher data quality, better reproducibility and deeper proteome coverage, leading to more accurate protein identification and quantification.
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What is Protein Enrichment and Sample Cleanup?
Successful mass spectrometry (MS)-based proteomics depends on robust protein enrichment and sample cleanup strategies. These steps refine complex biological samples by eliminating interfering substances and selectively concentrating peptides for analysis. Common techniques such as precipitation, filtration, chromatography-based purification and affinity enrichment streamline bottom-up workflows, enhancing digestion efficiency and boosting peptide recovery. Optimizing these processes reduces variability and increases proteome depth, enabling you to uncover meaningful biological insights with higher confidence.
