Maxwell® HT DNA FFPE Isolation System

Ready-to-Go FFPE DNA Kit, Expert Support Available

The Maxwell® HT DNA FFPE Isolation System is compatible with automation on all major platforms, including Hamilton Star/Nimbus, Tecan Freedom Evo/Fluent, Biomek i5/i7, Eppendorf epMotion, and particle movers such as KingFisher.

When you purchase a Promega extraction solution, you gain free access to our elite Field Support Scientists, method development services, and technical support. Contact us if you need help setting up this FFPE DNA extraction system on your robot.

With decades of experience in building personalized automation solutions, we can help you customize the scale, application, and chemistry, regardless of platform, exactly to your needs—the only limit is your imagination.

Rapid Automated Extraction of FFPE DNA on Liquid Handlers

Simple, automation-friendly method

Not centrifugation or vacuum

No organic solvents

The Maxwell^® HT DNA FFPE Isolation System provides a simple and reliable method for high-throughput, rapid isolation of genomic DNA from FFPE (formalin-fixed, paraffin-embedded) tissue samples. The purified DNA can be used directly in a variety of downstream applications, including PCR and next-generation sequencing.

The Maxwell^® HT DNA FFPE Isolation System purifies nucleic acid using paramagnetic particles, which provide a mobile solid phase to optimize sample capture, washing and purification of gDNA.

FFPE System Performance Comparison

To demonstrate the performance of this system, Maxwell^® HT DNA FFPE purification chemistry was automated on the Tecan Freedom EVO^® and ThermoFisher KingFisher™ Flex instruments. Qiagen QIAamp^® and GeneRead kits were run using a standard method on a QIAcube instrument. Consecutive FFPE sections of human lung tumor and normal adjacent tissue (NAT) were interspersed among all methods and replicates to minimize sample-based variability. Eluates were quantitated using fluorometric detection and quality was assessed using the ProNex^® DNA QC Assay.

15035mb-w — **Yield of DNA Extracted from FFPE Tissues.** Extracted DNA was quantitated using the QuantiFluor® ONE dsDNA System to ensure high accuracy. For both tumor and normal tissue, Maxwell® HT DNA FFPE chemistry yielded consistently greater amounts of DNA than other systems.

15034ma — **Amplifiability of fragments of various sizes using qPCR.** After extraction, the ProNex^® DNA QC Assay was used to assess the amplifiability of the DNA using three particular targets as an indication of the DNA integrity and thus suitability for downstream applications, including qPCR and next-generation sequencing. In both sample types and at all amplicon lengths, eluates derived from the Maxwell^®HT DNA FFPE Isolation System were shown to provide greater amplification.

Tissue	Instrument	Uniformity of Coverage (%)	Mean Amplicon Coverage (X)	Reads (millions)
Tumor	Tecan	100.0	4818	1.08
Tumor	KingFisher	99.19	6036	1.28
Normal Adjacent	Tecan	100.0	5202	1.18
Normal Adjacent	KingFisher	100.0	4074	1.05

Next-Generation Sequencing Performance of DNA Purified with Maxwell® HT Chemistry. DNA purified using Maxwell® HT chemistry on both Tecan and KingFisher instruments was sequenced using an Illumina MiSeq after enrichment using the Ion AmpliSeq™ Cancer Hotspot Panel v2. Coverage, uniformity and depth were extremely high for both methods and for both tissue types, showing the suitability of these eluates in NGS applications. Furthermore, subsequent variant analysis revealed that low-frequency variants in the KRAS and EGFR genes were detected in the tumor tissue samples, but not in the normal tissue samples.

Automated DNA Extraction: FFPE Data

These application notes contain protocols and example data using the Maxwell® HT DNA FFPE Isolation System to purify DNA from formalin-fixed, paraffin-embedded tissue samples using the Kingfisher™ Flex system.

Your Purification Solution, Simplified

Our team of automation experts will help you choose an optimized high-throughput solution that meets your laboratory’s needs. We can implement Maxwell® HT chemistries on a variety of sample types and instrumentation platforms. Contact us for a consultation about choosing the right purification chemistry or customizing a purification chemistry for additional sample types.

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Protocolos

Complete Protocol

Maxwell HT DNA FFPE Isolation System Technical ManualPDF (1125 KB) – English

Contenido

Item	Part #	Presentación	Concentración	Available Separately
FFPE Resin	A220G	1 × 33ml
Mineral Oil	A244C	1 × 140ml
Lysis Buffer (LBA)	A287A	1 × 110ml
Buffer B (BWB)	A288B	2 × 58.8ml
Proteinase K (PK) Solution	A505E	1 × 30ml
Buffer A (BWA)	A6371	1 × 125ml		View Product
RNase A Solution	A797F	1 × 24ml	4mg/ml

SDS

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Certificado de Análisis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Condiciones de Almacenaje

Recursos

Artículos

Comparing Manual and Automated Genomic DNA Purification Methods for Genotyping Arrays

Otros

Lab Automation Resource Center

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High-Throughput Extraction of DNA from FFPE Samples

Ready-to-Go FFPE DNA Kit, Expert Support Available