Maxwell® RSC Plant DNA Kit

Maxwell RSC Plant DNA Kit 48 preps
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Automated DNA Extraction from Plant Tissues

  • Extracts DNA from a range of plant tissues, including soybean, corn and Arabidopsis
  • Consistent purification, no organic extractions and minimal preprocessing
  • Purified DNA is ready to use in downstream applications including amplification assays

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Catalog number selected: AS1490

$ 281.00
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Maxwell® RSC Plant DNA Kit
48 preps
$ 281.00
Your price: Acceder

Automated DNA Extraction from Plant Tissue

The Maxwell® RSC Plant DNA Kit is used with the Maxwell® RSC and RSC 48 Instruments to provide an easy method for efficient, automated purification of genomic DNA (gDNA) from plant tissue samples. The Maxwell® RSC Instrument can process from 1 to 16 samples, and the Maxwell® RSC 48 can process from 1 to 48 samples in a single run. 

Using this system, DNA can be purified from plant samples in under 60 minutes with minimal preprocessing and no organic extractions. Automated purification results in consistent purification with less variability than traditional DNA extraction methods such as CTAB and spin-columns.

The Instruments are supplied with preprogrammed purification methods and use predispensed reagent cartridges, maximizing simplicity and convenience.

Purified DNA Shows Minimal Inhibition in Downstream Assays

DNA purified with the Maxwell® RSC Plant DNA Kit is ready for use in downstream applications, including qPCR. PCR using DNA purified with the Maxwell® RSC Plant DNA Kit shows no significant evidence of inhibitors.

In this experiment, tenfold dilutions of DNA purified from 20mg of various plant leaf tissues were amplified using qPCR. The ΔCq is calculated as the shift in Cq between each tenfold dilution. The expected ΔCq value for a tenfold dilution is around 3.3. The observed ΔCq value for the Maxwell® RSC Plant DNA samples indicates minimal inhibition.


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High DNA Yield from Various Plant Samples

The cellulose-based particles in the Maxwell® Kits have higher binding capacity than silica-based DNA purification methods, giving consistently high yields. In the samples shown here, DNA was isolated from 20mg of plant tissues (Arabidopsis, Balsam, Corn and Soybean). The eluted DNA was quantified using the QuantiFluor® dsDNA Dye and the Quantus™ Fluorometer.

13432MA-W

Protocolos

Complete Protocol

Protocolos Rápidos

Especificaciones

Contenido

Item Part # Presentación Concentración Available Separately

Tail Lysis Buffer (TLA)

 A509C 1 × 25ml View Product

RNase A Solution

 A797C 1 × 1ml 4mg/ml View Product

Elution Buffer

 A828D 1 × 20ml View Product

Maxwell® RSC Cartridge (RSCB)

 AS140A 48 × 1 each

Elution Tubes (0.5ml)

 AS620A 1 × 50/pk View Product

Nuclease-Free Water

 P119C 1 × 25ml View Product

RSC Plungers

 AS1670 1 × 48/pack View Product

SDS

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Certificado de Análisis

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Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Condiciones de Almacenaje

DD

Patentes y Exclusiones

U.S. Pat. No. 6,855,499 and other patents.

Recursos

Software y Firmware

Posters

Productos Similares

Maxwell® RSC Plant RNA Kit

Extrae ARN a partir de muestras de tejido vegetal, utilizando el Maxwell® RSC Instrument.

AS1500

Maxwell® RSC PureFood GMO and Authentication Kit

Purificación segura y automatizada de ADN a partir de muestras de alimentos y piensos.

AS1600

Maxwell® 16 LEV Plant RNA Kit

Extracción de ARN a partir de muestras de tejido vegetal. Procesa de 1-16 muestras en 60 minutos, utilizando el Maxwell® Instrument.

AS1430

Usado con frecuencia junto con

Maxwell® RSC Instrument

Instrumento para la purificación automatizada de ácidos nucleicos, con cuantificación integrada. Sólo para uso en investigación.

AS4500

Quantus™ Fluorometer and NGS Starter Package

Dos canales de fluorescencia para la cuantificación de ácidos nucleicos y proteínas. Disponible en paquete para aplicaciones NGS.

E6150, E5150

QuantiFluor® dsDNA System

Sistema de cuantificación sensible y específico de ADN de doble hélice.

E2670, E2671

Maxwell® RSC 48 Instrument

Instrumento para la purificación automatizada de ácidos nucleicos de 1 a 48 muestras. Sólo para uso en investigación.

AS8500

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