Maxwell® RSC XtractAll FFPE DNA/RNA Kit
Flexible and Fast DNA and RNA Purification from FFPE Tissue Samples
- Extract DNA, RNA, total nucleic acid, or sequentially extract DNA and RNA from FFPE samples to maximize sample inputs
- Save time with minimal hands-on effort to obtain both DNA and RNA in ~180 minutes
- Consistently achieve excellent yield and quality of DNA and RNA suitable for sensitive amplification assays, including ddPCR and sequencing
- Quantify with confidence; fluorescence-based measurements align with downstream assay performance
Catalog Number:
Size
Flexible and Efficient Purification of DNA and RNA from FFPE Samples
The Maxwell® RSC XtractAll FFPE DNA/RNA Kit, used with the Maxwell® RSC or Maxwell® RSC 48 Instrument, is an innovative solution designed to efficiently purify DNA and RNA from formalin-fixed, paraffin-embedded (FFPE) tissue samples. This kit streamlines nucleic acid extraction with unparalleled flexibility by offering four distinct workflows allowing users the choice to extract: DNA only, RNA only, total nucleic acid in a single eluate, or sequentially extract DNA and RNA into separate eluates—all from the same sample. This versatility ensures that laboratories can maximize the utility of limited FFPE tissue inputs. The Maxwell® RSC XtractAll FFPE DNA/RNA Kit combines minimal hands-on time and easy-to-follow protocols, with the consistency and efficiency of automated extraction. The Maxwell® RSC XtractAll FFPE DNA/RNA Kit produces high-quality nucleic acids that are suitable for advanced downstream analysis assays, such as digital PCR and next-generation sequencing (NGS).
The Maxwell® RSC Instrument can process 1 to 16 samples, and the Maxwell® RSC 48 Instrument can process 1 to 48 samples, in a single run. The Maxwell® Instruments are supplied with preprogrammed purification procedures and are designed for use with the predispensed reagent cartridges provided in the Maxwell® RSC XtractAll FFPE DNA/RNA Kit.
Note: The Maxwell® RSC XtractAll FFPE DNA/RNA Kit is only compatible with Maxwell® RSC or Maxwell® CSC (RUO mode) Instrument software version 4.0.0 or greater; and Maxwell® RSC 48 or Maxwell® CSC 48 (RUO mode) Instrument software version 4.1.0 or greater.
Maxwell® RSC XtractAll FFPE DNA/RNA Kit workflows. After preparing the FFPE tissue sample lysate, choose to extract DNA, RNA, total nucleic acid, or sequentially extract DNA and RNA. Only one cartridge per sample is needed regardless of the workflow you choose. This kit maximizes flexibility and utilization of limited FFPE samples, while minimizing waste.
Fast and Versatile FFPE DNA and RNA—Without Compromises!
The Maxwell® RSC XtractAll FFPE DNA/RNA Kit significantly reduces sample extraction turnaround time with workflows of ~180 minutes or less, depending on the selected protocol. With this kit, FFPE tissue sample lysates are prepared in approximately 90 minutes, and then the remaining extraction steps are automated by the Maxwell® Instrument for fast, consistent and efficient extraction in only 30–60 minutes. This streamlined approach enables laboratories to reliably and quickly process samples, freeing up valuable time for other laboratory needs.
Comparison of FFPE DNA and RNA extraction protocols. The Maxwell® RSC XtractAll FFPE DNA/RNA Kit can extract both DNA and RNA sequentially in less than half the time compared to other sequential DNA/RNA extraction kits.
The Maxwell® RSC XtractAll FFPE DNA/RNA Kit delivers unmatched flexibility with four workflow options. From one FFPE input sample, choose to extract: DNA only, RNA only, total nucleic acid in a single eluate, or sequential DNA and RNA extraction into separate eluates. This adaptability supports a diversity of applications without compromising on yield or quality of results, eliminating the need to perform multiple extractions and benefiting laboratories with limited FFPE sample material.
The Maxwell® RSC XtractAll FFPE DNA/RNA Kit provides consistent yield of DNA and RNA in each workflow. Lung tumor FFPE (Panel A) and colon normal FFPE (Panel B) samples were extracted using the Maxwell® RSC XtractAll FFPE DNA/RNA Kit following the DNA (“DNA”), RNA (“RNA”), total nucleic acid (“TNA”) or sequential DNA and RNA (“Seq DNA” and “Seq RNA”) protocols. The yield of DNA and RNA was evaluated via amplification assays (102bp target for DNA, 100bp target for RNA). Data represent mean ±SD, N=6.
High-Quality DNA and RNA for Advanced Downstream Analysis Assays
The Maxwell® RSC XtractAll FFPE DNA/RNA Kit delivers high-quality DNA and RNA from a variety of tissue types. The nucleic acids extracted using this kit demonstrate excellent integrity and high yields of DNA and RNA, especially for longer fragment lengths as compared to other FFPE extraction offerings.
Comparison of DNA and RNA yields from a variety of FFPE tissue samples. DNA and RNA were extracted from multiple types of FFPE tissue samples (breast, liver, colon, kidney, uterine, endometrial, lung, cervical) using the Maxwell® RSC XtractAll FFPE DNA/RNA Kit sequential DNA/RNA workflow and two other commercially available sequential FFPE DNA/RNA extraction kits. DNA yield was evaluated by amplification using the ProNex® DNA QC Assay (Cat.# NG1002) for short (75bp; Panel A) and long (300bp; Panel B) target amplicons. RNA was evaluated by amplification assay for short (100bp; Panel C) and long (171bp; Panel D) target amplicons. The Maxwell® RSC XtractAll FFPE DNA/RNA Kit shows equivalent or superior performance for both DNA and RNA yields when evaluated by amplification assay, especially for longer target amplicons. Data represent mean ±SD, N=6.
Comparison of FFPE DNA and RNA fragment size and quality from several extraction kits. DNA and RNA were extracted from breast FFPE tissue samples using the Maxwell® RSC XtractAll FFPE DNA/RNA Kit sequential DNA/RNA workflow and two other commercially available sequential FFPE DNA/RNA extraction kits. Panel A. DNA fragment size was evaluated using the Agilent TapeStation D5000 ScreenTape Assay. Panel B. RNA fragment size was evaluated using the Agilent TapeStation RNA ScreenTape Assay, and DV200 values are shown.
The high quality and consistency of nucleic acids extracted with the Maxwell® RSC XtractAll FFPE DNA/RNA Kit makes this kit an ideal choice for demanding applications and sensitive assays, such as digital PCR or NGS.
Performance of the Maxwell® RSC XtractAll FFPE DNA/RNA Kit in digital PCR. RNA was extracted from lung tumor FFPE samples using the Maxwell® RSC XtractAll FFPE DNA/RNA Kit and tested in EML4-ALK mRNA Fusions Assay (Bio-Rad). A 2D amplitude plot is shown; successful detection of the positive gene fusions is identified.
Reliable Fluorescent Quantitation for Confident Downstream Assay Performance
Fluorescence dye-based quantitation assays, such as the QuantiFluor® ONE dsDNA System (Cat.# E4871) and QuantiFluor® RNA System (Cat.# E3310), are often used to quickly and conveniently assess the yield of extracted DNA and RNA. In contrast to other FFPE extraction offerings, DNA and RNA extracted with the Maxwell® RSC XtractAll FFPE DNA/RNA Kit produces fluorescence dye quantitation results that more reliably predict success of the purified nucleic acids in amplification assays, such as qPCR and digital PCR. This improved concordance between fluorescence dye quantitation and amplification assays minimizes the risk of failed downstream assays, saving you both time and money.
Assessment of fluorescence- and amplification-based quantitation assay concordance for DNA and RNA extracted from FFPE tissue. DNA and RNA were extracted from eight different FFPE tissue samples using the Maxwell® RSC XtractAll FFPE DNA/RNA Kit and two other commercially available sequential FFPE DNA/RNA extraction kits. Panel A. DNA was quantified using fluorescence-based (QuantiFluor® ONE dsDNA System [Cat.# E4871]) and amplification-based (qPCR; 102bp amplicon) assays. Panel B. RNA was similarly quantified using fluorescence-based (QuantiFluor® RNA System [Cat.# E3310]) and amplification-based (RT-qPCR, 100bp amplicon) assays. The ratio of fluorescence- to amplification-based quantification was calculated and compared (“Fluorescence/Amp Ratio”). A ratio value approaching 1 indicates good concordance of the results between the fluorescence-based and amplification-based quantitation assays. In contrast to the other extraction kits, the Maxwell® RSC XtractAll FFPE DNA/RNA Kit demonstrated fluorescence-based quantitation readouts that more consistently predict DNA and RNA quantitation via amplification-based assays.
Protocols
Complete Protocol
Protocolos Rápidos
Specifications
Catalog Number:
Contenido
| Item | Part # | Presentación | Available Separately |
|---|---|---|---|
Maxwell® RSC Cartridge (RSCR) |
AS156A | 48 × 1 each | |
Elution Tubes (0.5ml) |
AS620A | 2 × 50/pk | View Product |
Proteinase K (PK) Solution |
MC111A | 2 × 1ml | |
Lysis Buffer |
MC118A | 1 × 20ml | |
Nuclease-Free Water |
MC119A | 1 × 25ml | |
Blue Dye |
MC122A | 2 × 100μl | |
MnCl2, 0.09M |
MC125A | 2 × 1ml | |
|
DNase I (lyophilized) |
Z358A | 3 × 1 vial | View Product |
RSC Plungers |
AS1670 | 1 × 48/pack | View Product |
SDS
Search for SDSCertificado de Análisis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Condiciones de Almacenaje
Resources
Software y Firmware
Related Products
Productos Similares
ReliaPrep™ FFPE gDNA Miniprep System
Purificación rápida y manual de ADN a partir de tejidos FFPE, sin utilizar disolventes orgánicos.
A2351, A2352
ReliaPrep™ FFPE Total RNA Miniprep System
ARN total amplificable, purificado a partir de tejidos FFPE sin disolventes orgánicos ni digestión durante la noche.
Z1001, Z1002
Maxwell® HT DNA FFPE Isolation System
Purificación de alto rendimiento de ADN a partir de muestras FFPE.
A6372
Maxwell® RSC RNA FFPE Kit
Purifica ARN a partir de secciones de tejido FFPE, utilizando el Maxwell® RSC Instrument.
AS1440
Maxwell® RSC DNA FFPE Kit
Extrae ADN a partir de muestras de tejido FFPE, utilizando el Maxwell® RSC Instrument.
AS1450, ASB1450
Maxwell® RSC Xcelerate DNA FFPE Kit
An expedited process (<1 hour) for purifying DNA from FFPE tissue samples.
AS1510
Maxwell® RSC FFPE Plus DNA Kit
Método rápido para la extracción automatizada de ADN a partir de 1-16 (5µm) muestras FFPE. Requiere el Maxwell® 16 o Maxwell® RSC Instrument.
AS1135, AS1720, AS1770
Usado con frecuencia junto con
Maxwell® RSC Instrument
Instrumento para la purificación automatizada de ácidos nucleicos, con cuantificación integrada. Sólo para uso en investigación.
AS4500
Maxwell® RSC 48 Instrument
Instrumento para la purificación automatizada de ácidos nucleicos de 1 a 48 muestras. Sólo para uso en investigación.
AS8500
QuantiFluor® ONE dsDNA System
Cuantificación "add-and-read" de ADN de doble cadena. Incluye un dye de unión al ADN, lambda ADN y un tampón.
E4871, E4870
QuantiFluor® RNA System
Cuantificación fluorescente de pequeñas cantidades de ARN en solución.
E3310
